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Production method and device for butanol by fermentation coupled separation and purification

A technology for purifying butanol and butanol, applied in the biological field, can solve the problems of low separation efficiency and low concentration of separated products, and achieve the effects of solving membrane pollution, saving purification energy consumption, and low energy consumption

Pending Publication Date: 2017-12-26
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main problem of using these separation technologies for separation is low separation efficiency and low concentration of separated products. Further optimization of the technology and dehydration of the products are still required.

Method used

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  • Production method and device for butanol by fermentation coupled separation and purification
  • Production method and device for butanol by fermentation coupled separation and purification
  • Production method and device for butanol by fermentation coupled separation and purification

Examples

Experimental program
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Embodiment

[0063] The present invention will be specifically described below in conjunction with the examples by taking the acetone-butanol-producing bacteria as an example. However, the present invention is not limited to the following examples, and the present invention can be appropriately modified within the range consistent with the purpose of the present invention. In addition, in the following examples, unless otherwise specified, the experimental methods used are conventional methods, and the materials and reagents used can be purchased from biological or chemical companies.

[0064] Acetobutanol-producing bacteria: Clostridium acetobutylicum (Clostridium acetobutylicum), purchased from the American ATCC strain bank (ATCC number: 55025-E604).

[0065] The pretreatment method of corn stalks: crush the corn stalks, pass through a 0.4mm sieve, add 200g of crushed and sieved corn stalks to 2L of 2% (w / v) NaOH solution, react at 120°C for 30min, cool to room temperature, Filter, wash...

Embodiment 1

[0082] Example 1 Production of butanol, acetone and ethanol by gasification-osmotic coupled fermentation with glucose as carbon source

[0083] The cultivation and fermentation of the acetone-butanol-producing bacteria were carried out as described above. After the production bacteria are inserted into the bioreactor, fermentation begins. When the butanol concentration in the fermentation broth reaches 5g / L, start the pump between the bioreactor and the membrane separation device, and bubble into the bioreactor, the pump makes the gas and the gasified butanol and acetone entrained by the gas And ethanol circulates in the closed space formed by the bioreactor and the membrane separation device. When the vaporized butanol, acetone and ethanol pass through one side of the separation membrane, they will dissolve and diffuse to the other side of the membrane, and be condensed and recovered . When the glucose concentration in the fermentation medium drops below 10g / L, add 400g / L o...

Embodiment 2

[0084] Example 2 Production of butanol, acetone and ethanol by gasification-osmosis coupled fermentation using corn stalk hydrolyzate as carbon source

[0085] The cultivation and fermentation of the acetone-butanol-producing bacteria were carried out as described above. After the production bacteria are inserted into the bioreactor, fermentation begins. When the butanol concentration in the fermentation broth reaches 5g / L, start the pump between the bioreactor and the membrane separation device, and bubble into the bioreactor, the pump makes the gas and the gasified butanol and acetone entrained by the gas And ethanol circulates in the closed space formed by the bioreactor and the membrane separation device. When the vaporized butanol, acetone and ethanol pass through one side of the separation membrane, they will dissolve and diffuse to the other side of the membrane, and be condensed and recovered . When the reducing sugar concentration in the fermentation medium drops be...

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Abstract

The invention relates to a method for fermentation coupled separation and purification of butanol, acetone and ethanol or purification of butanol. The method includes the steps of: 1) fermenting acetone butanol producing strain to obtain butanol, acetone and ethanol or utilizing a butanol producing strain to obtain butanol; and 2) conducting on-line separation and purification of butanol, acetone and ethanol or purification of butanol from the fermentation broth by vaporization penetration, wherein the vaporization penetration process includes the steps of: introducing bubbles into living cell-containing fermentation broth just under fermentation to vaporize butanol, acetone and ethanol or butanol; letting gas entraining vaporized butanol, acetone and ethanol or vaporized butanol in the bubbles enter a membrane separation device and penetrate the membrane; and recovering the butanol, acetone and ethanol or butanol, or entering a next stage separation device. The method provided by the invention effectively improves the production and separation purification efficiency of butanol, acetone and ethanol or butanol without increasing equipment investment and saving purification energy consumption.

Description

technical field [0001] The invention relates to a method for producing butanol, acetone and ethanol (or butanol) through fermentation coupling separation and purification, and belongs to the field of biotechnology. Background technique [0002] Butanol and acetone, as potential liquid energy sources and important chemicals used in medicine and food industries, can be obtained through microbial fermentation. For details, see the literature: Dürre, P.Biobutanol: anattractive biofuel.Biotechnol.J.2: 1525– 1534, 2007. However, when using Clostridium acetobutylicum or Clostridium beijerinckii to produce butanol by fermentation, the end-point butanol concentration in the fermentation broth usually does not exceed 2.0% (w / v). In addition, the boiling point of butanol is 117.7°C, which is 100°C higher than the boiling point of water. Therefore, if traditional rectification or distillation separation method is utilized, its separation cost is extremely high, economically it is not ...

Claims

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Application Information

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IPC IPC(8): C12P7/16C12P7/28C12P7/06C07C45/78C07C49/08C07C29/76C07C31/08C07C31/12C12M1/00C12M1/04C12R1/145C12R1/19
CPCC07C29/76C07C45/786C12M29/06C12M47/18C12P7/06C12P7/16C12P7/28C07C49/08C07C31/08C07C31/12Y02E50/10Y02P20/10Y02P20/50Y02P20/59
Inventor 薛闯朱超陈丽杰
Owner DALIAN UNIV OF TECH
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