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Production technology of porcine red blood cell peptide

A production process and technology of pig blood cells, applied in the field of hemoglobin preparation, can solve the problems of reduced product activity, slow enzymatic hydrolysis, loss of porphyrin iron, etc., and achieve the effects of maintaining activity, reducing product loss, and reducing energy consumption

Inactive Publication Date: 2018-01-02
湖北瑞邦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies in the prior art, the present invention provides a production process of porcine blood globulin, which solves the problem of slow enzymolysis and low efficiency caused by unbroken blood cells in the traditional production process of porcine blood globulin Leading to the reduction of product activity and the loss of small molecular peptides and porphyrin iron caused by decolorization of activated carbon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of production technology of pig blood globulin of the present invention, comprises the following steps:

[0034] a. Collection: collect fresh pig blood that has passed the quarantine inspection;

[0035] b. Primary centrifugation: the fresh pig blood in step a is subjected to primary centrifugation using a high-speed centrifuge with a rotating speed of 3500r / min to obtain blood cell liquid containing blood cells and plasma;

[0036] c. Broken: the blood cell fluid in step b is added into an equal volume of purified water and then processed by freeze-thaw method. The freeze-thaw method includes a freezing step and a thawing step. The freezing step includes placing the blood cell fluid after adding purified water at -20 Freezing for 12 hours in an environment of °C, the thawing step includes placing the blood cell solution in the freezing step in an environment of 25°C for processing to break the blood cells to obtain a hemolyzed solution;

[0037] d. Enzymolysis:...

Embodiment 2

[0043] A kind of production technology of pig blood globulin of the present invention, comprises the following steps:

[0044] a. Collection: collect fresh pig blood that has passed the quarantine inspection;

[0045] b. Primary centrifugation: the fresh pig blood in step a is subjected to primary centrifugation using a high-speed centrifuge with a rotating speed of 3000r / min to obtain blood cell liquid containing blood cells and plasma;

[0046] c. Broken: the blood cell fluid in step b is added into an equal volume of purified water and then processed by freeze-thaw method. The freeze-thaw method includes a freezing step and a thawing step. The freezing step includes placing the blood cell fluid after adding purified water at -20 Freezing for 10 hours in an environment of ℃, the thawing step includes placing the blood cell solution in the freezing step in an environment of 20°C for processing, breaking the blood cells to obtain a hemolyzed solution;

[0047] d. Enzymolysis:...

Embodiment 3

[0053] A kind of production technology of pig blood globulin of the present invention, comprises the following steps:

[0054] a. Collection: collect fresh pig blood that has passed the quarantine inspection;

[0055] b. Primary centrifugation: a high-speed centrifuge with a rotational speed of 2500r / min is used to perform primary centrifugation on the fresh pig blood in step a to obtain blood cell liquid containing blood cells and plasma;

[0056] c. Broken: the blood cell fluid in step b is added into an equal volume of purified water and then processed by freeze-thaw method. The freeze-thaw method includes a freezing step and a thawing step. The freezing step includes placing the blood cell fluid after adding purified water at -20 The freezing treatment of 8°C is carried out, and the thawing step includes placing the blood cell solution in the freezing step in an environment of 15°C for processing to break the blood cells to obtain a hemolyzed blood cell solution;

[0057]...

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PUM

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Abstract

The present invention proposes a production process of porcine blood globulin, comprising the following steps: a, collection; b, primary centrifugation; c, crushing; d, enzymatic hydrolysis; e, secondary centrifugation; f, enzyme inactivation; g, concentration; h , freeze-drying. Use the freeze-thaw method to promote the rupture of the hydrophobic bond structure of the cell membrane of the blood cell (blood cell), enhance its hydrophilicity, and the intracellular water forms ice crystals to burst the blood cell, achieving the effect of breaking the blood cell. In the subsequent enzymatic hydrolysis step, it can speed up the enzymatic hydrolysis Speed, improve the efficiency of enzymatic hydrolysis, reduce product loss.

Description

technical field [0001] The invention relates to the technical field of preparation of blood globulin, in particular to a production process of porcine blood globulin. Background technique [0002] The development of animal blood in foreign countries began at the beginning of the last century, and it was mainly used in the feed industry in the early stage. With the development of high technology, many research departments at home and abroad pay special attention to the research of processing animal blood with biochemical technology for food and pharmaceuticals. At present, blood products have formed a certain scale in the health care products industry, and the industrialization prospect is good. The production process commonly used in animal blood globulin peptides is as follows: Animal blood—centrifugal separation—blood cells—enzyme hydrolysis—enzyme inactivation—pH adjustment—centrifugation—supernatant—decolorization—filtration—pH adjustment—deployment—sterilization—bottli...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 尹晓清靳祯亮王希搏吴海刘雅颀
Owner 湖北瑞邦生物科技有限公司
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