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Ganoderma lucidum component GL-1 and application thereof to aspect of estrogen replacement

A technology of estrogen and GL-1, which is applied in the application field of traditional Chinese medicine ingredients, can solve the problems that affect the development and utilization of Ganoderma lucidum, it is difficult to study deeply in pharmacology, and the number of compounds is not enough.

Inactive Publication Date: 2018-01-05
CHINA THREE GORGES UNIV
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AI Technical Summary

Problems solved by technology

However, some research works only use the small platform of the laboratory to carry out extraction and separation work, resulting in insufficient number of isolated compounds. Therefore, it is difficult to carry out in-depth research on pharmacology and drug metabolism in vivo, only in chromatographic analysis and Some studies have been carried out in molecular structure identification, which greatly influenced the continued development and utilization of Ganoderma lucidum

Method used

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  • Ganoderma lucidum component GL-1 and application thereof to aspect of estrogen replacement
  • Ganoderma lucidum component GL-1 and application thereof to aspect of estrogen replacement
  • Ganoderma lucidum component GL-1 and application thereof to aspect of estrogen replacement

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Embodiment 1

[0023] Drugs and Cells

[0024] GL-1 (provided by the State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, isolated from Ganoderma lucidum. Purity 98%); 17β-estradiol (purchased from Sigma); human breast cancer cell lines MCF-7, MDA-MB -231 (purchased from Beijing Type Culture Collection Center, China).

[0025] main reagent

[0026] DMEM dry powder (purchased from Gibco Company), L-15 dry powder medium (purchased from Invitrogen Corporation); fetal bovine serum, calf serum (purchased from Zhejiang Tianhang Biotechnology Co., Ltd.); thiazolyl blue (MTT) (purchased from the United States Amresco); trypsin (purchased from Sigma); ERα, ERβ monoclonal antibodies (purchased from Abcam, USA); β-actin monoclonal antibodies (purchased from Cell Signaling, USA); secondary antibodies (purchased from Shanghai You Ningwei Biotechnology Co., Ltd.); amino-free yeast nitrogen source (Difco) (purchased from Angel Yeast Co., Ltd.); dimethyl sulfoxide DMSO...

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Abstract

The application discloses estrogen effects of a Ganoderma lucidum component GL-1 and preliminary discussion of an action mechanism thereof. A computer-assisted simulation computation is used for computing binding capability of GL-1 and an estrogen acceptor, a MTT method is used for detecting influences of GL-1 on proliferation of MCF-7 cells and MDA-MB-231 cells, influences of GL-1 on proliferation of MCF-7 cells induced by estrogen as well as influences of an estrogen acceptor inhibitor ICI182,780 on proliferation of MCF-7 cells induced by GL-1; Western blot is used for detecting expression conditions of ER alpha and ER beta monoclonal antibodies. Results show that the Ganoderma lucidum component GL-1 and an estrogen acceptor beta have good combination capability, and Ganoderma lucidum component GL-1 has estrogen-like effects, can be combined with the estrogen acceptor, and influences estrogen secretion and ER beta expression.

Description

technical field [0001] The invention provides an application of traditional Chinese medicine components, specifically ganoderma component GL-1 and its application as estrogen. Background technique [0002] Estrogen is a female hormone produced by the ovaries and placenta, and to a lesser extent by the adrenal cortex. After entering puberty, the ovaries of female children will also secrete estrogen during this period. At the same time, the endometrium hyperplasia. In addition, this estrogen can promote the development of the vagina, fallopian tubes, uterus and ovaries themselves, thus causing menstrual cramps . Estrogen in women is secreted by the ovaries before menopause. After entering the bloodstream, these estrogens, secreted by the ovaries, circulate in the body. Therefore, estrogen forms distant targets in some parts, such as uterus, bone, blood vessels, heart and brain, so as to play a coordinated role. Circulating estradiol levels in the blood fluctuate during the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/58A61K31/341A61P5/30A61P15/00A61P35/00
Inventor 汪鋆植王爱玲刘洪伟罗发军李明黄年玉余海立叶红
Owner CHINA THREE GORGES UNIV
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