Preparation and application of a poultry immune enhancer

An immune enhancer, poultry technology, applied in the field of bioengineering and biology, can solve the problem of lack of safe, efficient, green new immune enhancer, etc., to achieve the effect of improving the immune organ index, enhancing the proliferation and differentiation ability, and enhancing the growth of immune organs

Active Publication Date: 2020-05-05
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current lack of safe, efficient, and green new-type immune enhancers, the object of the present invention is to provide an application of the fermentation product of Inonotus obliquus as an immune enhancer in enhancing the immune performance of poultry

Method used

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  • Preparation and application of a poultry immune enhancer
  • Preparation and application of a poultry immune enhancer
  • Preparation and application of a poultry immune enhancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Isolation and identification of Inonotus obliquus.

[0047] Inonotus obliquus YU strain is a wild strain collected from Dali City, Yunnan Province. When collecting, the fruiting bodies free from diseases and insect pests are removed from the surface of the tree, the depth of collection is 1-3 cm from the surface of the tree, and they are packed into a specimen box and returned to the laboratory for the next step of separation and purification. To isolate the strains, the toadstool separation method is used. First, distilled water is used to remove sawdust and impurities on the surface of the fruit body, and then 75% ethanol is used to disinfect the surface. Into the tiger red solid medium, placed in a constant temperature incubator (28 ° C) for 3 days, and then the purified bacteria were transferred to the PDA solid medium, and the growth of the colony was observed every day. Then, the Inonotus obliquus genome extracted by the improved CTAB method was used as...

Embodiment 2

[0048] Example 2 Obtaining of Fermentation Products of Inonotus obliquus.

[0049] Preparation of liquid medium: according to the amount of liquid medium, weigh the following components (g / L): peptone 10, glucose 15, potassium dihydrogen phosphate 1, magnesium sulfate 0.5, vitamin B 10.1, soybean oil 0.2, make up to the required volume with sterilized water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, and set aside.

[0050] (1) Cultivation of mother species: inoculate the preserved strains of Inonotus obliquus into potato dextrose agar medium (PDA) on a test tube slope, transfer twice, activate each time at 25°C for 7 days, and become the mother species. spare;

[0051] (2) Shake flask culture: transfer the mother bacteria to 500mL primary culture medium and cultivate for 10 days, then transfer to secondary culture medium and continue to cultivate for 5 days. Take a small amount of culture for microscopic examination, no bacteria to enter the next step;

[005...

Embodiment 3

[0057] Example 3 Immunity enhancement effect of the fermented product of Inonotus obliquus on poultry.

[0058] Astragalus polysaccharide is a well-researched and widely used traditional Chinese medicine extract, which can significantly improve the immunity of animals. In order to better compare the effect of the fermented product of Inonotus obliquus on enhancing the immune performance of poultry, astragalus polysaccharide was selected as the positive control, and the effects of the two preparations were compared. Animal experiments were divided into 7 groups (Ⅰ-Ⅶ), 20 1-day-old SPF chickens in each group, which were raised in isolators. Groups Ⅰ-Ⅴ were fed feed containing 0.2%, 0.4%, 0.8%, 1%, 2% of the fermentation product of Inonotus obliquus in Example 2, and group VI was the blank control group (no immune enhancer was added to the feed) , Group VII was fed with feed containing 0.2% astragalus polysaccharide (purchased from Zentel, the recommended dosage was 0.2%). The ...

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Abstract

The invention provides a poultry immunopotentiator. The poultry immunopotentiator comprises a fermentation product of inonotus obliquus which is preserved in the China General Microbiological CultureCollection Center with a preservation number of CGMCC No.14549. Adding amount, in poultry feed, of the fermentation product of the inonotus obliquus is 0.2-2wt%; and adding amount of poultry drinkingwater is 0.1-5wt%. The inonotus obliquus fermentation product is used to feed the poultry, so that immune organ growth, non-specific immune response and specific immune response of the body can be remarkably improved; and immune organ indexes are increased, non-specific immune related indexes are increased, peripheral blood lymphocyte proliferation and differentiation ability is improved, antibodyand neutralizing antibody titer is increased, content of cell factors is increased, so that a novel tool is provided for preventing and controlling poultry diseases.

Description

technical field [0001] The invention belongs to the fields of bioengineering and biotechnology, and in particular relates to the fermentation technology of the fungus Inonotus obliquus and the application of the fermentation product in enhancing poultry immunity. Background technique [0002] With the improvement of people's living standards, the quality requirements for animal products are increasing day by day. In recent years, food safety issues have emerged continuously, which has made the whole society pay more attention to the safety of animal-derived food than ever before. How to reduce or even eliminate the use of antibiotics, reduce poultry drug resistance and other toxic side effects under the background of continuous high incidence of poultry diseases, realize green and healthy poultry farming and food safety, and find safe, efficient, green new feed additives and immune enhancers instead Drugs have become the top priority in the development of the aquaculture ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A61K36/07A61P37/04C12R1/645
Inventor 张琳丁志勇吴家强李海燕于森
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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