Selaginella tamariscina tissue culture rapid propagation process technique

A technology of tissue culture rapid propagation and process technology, which is applied in the field of tissue culture rapid propagation of Selaginella, can solve the problems of reducing the output of medicinal materials, and achieve the effect of saving the amount of seeds used and increasing the output

Inactive Publication Date: 2018-01-16
罗荣棋
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, Selaginella is mainly propagated by seeds, but its medicinal parts

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Obtaining sterile seedlings: select full-bodied Selaginella seeds, peel off the seed coat with surgical scissors in an ultra-clean workbench, disinfect with 75% alcohol for 32 seconds, then disinfect with 0.1% mercury solution for 15 minutes, and finally use sterile water Rinse 5 times, blot the surface moisture with sterile filter paper and inoculate into seed germination medium. After inoculation, the germination rate reached 100% after being cultured for 28 days under the conditions of 28 hours of light per day, light intensity of 1500lx, culture temperature of 28°C, and relative air humidity of 75%. The germination medium is: 1 / 2MS+0.1mg / L NAA+20g / L sucrose+3.2g / L agar, with a pH of 5.6.

[0017] (2) Callus induction: When the cotyledons of the sterile seedlings have just unfolded, take the shoot tips of the sterile seedlings as explants, cut them into a length of about 0.3 cm with a scalpel, and inoculate them in the medium for callus induction to cultivate. ...

Embodiment 2

[0023] (1) Obtaining sterile seedlings: select full-bodied Selaginella seeds, peel off the seed coat with surgical scissors in an ultra-clean workbench, disinfect with 75% alcohol for 48 seconds, then disinfect with 0.1% mercury solution for 8 minutes, and finally use sterile water Rinse 8 times, blot the surface moisture with sterile filter paper and inoculate into seed germination medium. After inoculation, the germination rate reached 100% after being cultured for 22 days under the conditions of 26 hours of light per day, light intensity of 1600lx, culture temperature of 26°C, and relative air humidity of 75%. The germination medium is: 1 / 2MS+0.1mg / L NAA+19g / L sucrose+3.6g / L agar, with a pH of 5.6.

[0024] (2) Callus induction: When the cotyledons of the aseptic seedlings have just unfolded, take the shoot tips of the aseptic seedlings as explants, cut them into a length of about 0.3 cm with a scalpel, and inoculate them in the medium for callus induction to cultivate. A...

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PUM

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Abstract

The invention discloses a selaginella tamariscina tissue culture rapid propagation process technique. Selaginella tamariscina is a renascent herb; fertile spikes grow on tops of twigs and take the shape of a quadruple rim; sporophyl takes the shape of a triangle, long awns grow at the front end of the sporophyl, and wide membranes grow on the edge of the sporophyl; sporangia take the shape of a kidney, and large and small spores are irregularly arranged; the whole selaginella tamariscina can be taken as a medicine and can be harvested in both spring and autumn; the selaginella tamariscin growsin most area on rock mostly, is spicy in taste and gentle in property and has the effects of activating blood when taken without processing and stopping bleeding after being fried. According to the process technique, selaginella tamariscin seeds are adopted as explants, in-vitro selaginella tamariscin replants are successfully cultured through procedures of callus induction, proliferation, differentiation, rooting, seedling strengthening, transplanting and the like, a selaginella tamariscina tissue culture rapid propagation system is established, and the seed amount of culture production is reduced.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a technology for rapid propagation of Selaginella tissue culture. Background technique [0002] Selaginella is also known as: Huiyang grass, Wannian pine, resurrection grass, evergreen, iron fist, tiger claw. [0003] Selaginella perennial herb. The main stem is erect, with fibrous roots. Each branch is clustered, erect, curled up after drying, densely covered with tile-like leaves. The leaves are small, shaped, arranged alternately; the lateral leaves are lanceolate subulate, the base is keel-shaped, with long awns at the apex, the abaxial side is entire, wide membranous, and the adaxial side has a very narrow membranous margin with microserrations; the middle leaf Two rows, oval-lanceolate, with long awns at the apex, obliquely, the left and right sides are unequal, the margins are slightly serrated, and the midrib is sunken on the leaves. Sporangia spikes are born on the top of bran...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 罗荣棋
Owner 罗荣棋
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