Carboxylation chitosan-dithiothreitol-gold nanocluster and preparation method thereof

A technology of carboxylated chitosan and dithiothreitol, which is applied in the nanometer field, can solve the problems of limited thiol ligand molecules and limited applications, and achieve rapid preparation, large Stokes shift, and easy surface modification. Effect

Active Publication Date: 2018-01-16
FUJIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This results in very limited thiol ligand molecules that can be used to prepare water-soluble gold nanoclusters, which greatly limits its application in related fields.

Method used

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  • Carboxylation chitosan-dithiothreitol-gold nanocluster and preparation method thereof
  • Carboxylation chitosan-dithiothreitol-gold nanocluster and preparation method thereof
  • Carboxylation chitosan-dithiothreitol-gold nanocluster and preparation method thereof

Examples

Experimental program
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Effect test

example 1

[0033] 1 mL of 0.4 mol / L sodium hydroxide solution and 1.6 mL of 20 mg / mL chloroauric acid were mixed in advance, and then 7.4 mL of 50 mg / mL carboxylated chitosan solution and 10 mL of 0.1 mol / L dithiothreitol solution, shake well and place in a 37°C water bath for constant temperature reaction for 8 h to obtain carboxylated chitosan-dithiothreitol-gold nanocluster solution. The reaction solution changed from light yellow to colorless. The reacted gold nanocluster solution was dialyzed in double distilled water for 24 h with a dialysis bag with a molecular weight cut off of 3500 to obtain a purified carboxylated chitosan-dithiothreitol-gold nanocluster solution. The obtained purified carboxylated chitosan-dithiothreitol-gold nanoclusters are colorless and transparent liquids (see figure 1 A-c), intense red fluorescence was produced under UV light irradiation (see figure 1 B-f), UV-Vis spectrum has a strong characteristic absorption peak of gold nanoclusters at 290 nm wavele...

example 2

[0035] Pre-mix 1 mL of 0.4 mol / L sodium hydroxide solution and 1.6 mL of 20 mg / mL chloroauric acid, then add 7.4 mL of 0.1-100 mg / mL carboxylated chitosan solution and 10 mL of 0.1 mol / L dithiothreitol solution, shake well and place in a 37°C water bath for constant temperature reaction for 8 h to obtain carboxylated chitosan-dithiothreitol-gold nanocluster solution. Such as Figure 4 Shown, the fluorescence intensity value F of the solution at 650 nm 650It reached the maximum when the concentration of carboxylated chitosan was 50 mg / mL.

example 3

[0037] Pre-mix 1 mL of 0.4 mol / L sodium hydroxide solution and 1.6 mL of 20 mg / mL chloroauric acid, then add 7.4 mL of 50 mg / mL carboxylated chitosan solution and 10 mL of 0.01 ~0.2 mol / L dithiothreitol solution was shaken well and placed in a water bath at 37°C for constant temperature reaction for 8 h to obtain carboxylated chitosan-dithiothreitol-gold nanocluster solution. Such as Figure 5 Shown, the fluorescence intensity value F of the solution at 650 nm 650 It reaches the maximum when the concentration of dithiothreitol solution is 0.1 mol / L.

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Abstract

The invention discloses a carboxylation chitosan-dithiothreitol-gold nanocluster and a preparation method thereof. Dithiothreitol serves as a reducing agent and a coordination agent, carboxylation chitosan serves as a stabilizer, and a water-soluble gold nanocluster fluorescent material is obtained through one-step synthesis. The novel gold nanocluster fluorescent material preparation method has the advantages that preparation is rapid, simpleness and environmental friendliness are achieved, and the quantum yield is high. The synthesized carboxylation chitosan-dithiothreitol-gold nanocluster shows strong red fluorescence (the maximum emission wavelength is 650 nm), and has the advantages of being long in fluorescence service life (3.7 microseconds and 9.7 microseconds), high in stability,large in Stokes shift (365 nm) and the like.

Description

technical field [0001] The invention relates to a carboxylated chitosan-dithiothreitol-gold nano-cluster fluorescent material and a preparation method thereof, belonging to the field of nanotechnology. Background technique [0002] In recent years, fluorescent metal nanoclusters, especially gold nanoclusters (AuNCs), have attracted much attention as a new type of fluorescent nanomaterials. Gold nanoclusters refer to relatively stable molecular-level aggregates composed of several to hundreds of gold atoms under the protection of a certain molecular layer. Since its size is close to the Fermi wavelength of electrons (about 0.7nm), the continuous energy state properties split into discrete energy states, and molecular-like size-dependent effects appear. Gold nanocluster materials used as fluorescent probes have the advantages of good photophysical properties, large specific surface area, easy surface modification, and adjustable fluorescence properties. Therefore, they have b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24C09K11/02C09K11/58B82Y40/00
Inventor 陈伟邓豪华黄开源彭花萍刘银环
Owner FUJIAN MEDICAL UNIV
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