Method for extracting Tremella polysaccharide from acid buffer solution
An acid buffer solution and tremella polysaccharide technology, applied in the field of plant polysaccharide extraction, can solve the problems of enzyme reagent cost, filtration difficulty, high energy consumption, etc., and achieve the effects of low cost, improved extraction rate, and low energy consumption
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0033] The commercially available white fungus is dried, crushed and passed through a 90-mesh sieve. After adding 40ml of citric acid-sodium citrate buffer solution with a pH of 3.0 to 1g of white fungus powder, place it in a hot water bath at 100°C for leaching. After 5 hours, centrifuge the leaching material at a speed of 8000rpm for 8min to remove the residue. Add 4 times the volume of 90% ethanol solution to the supernatant, precipitate for 12 hours, centrifuge at 10,000 rpm for 8 minutes, remove the supernatant, and obtain Tremella polysaccharide precipitate.
[0034] The control test is to replace the citric acid-sodium citrate buffer solution with water as the extraction solvent, and the method is the same as above.
[0035] All the obtained white fungus polysaccharide precipitates were dissolved in 20ml of water, and the polysaccharide content in the solution was measured by the phenol-sulfuric acid method to be 15.79mg / ml. The calculated polysaccharide quality in the ...
Embodiment 2
[0037]The commercially available white fungus is dried, crushed and passed through a 80-mesh sieve. After adding 40ml of citric acid-sodium citrate buffer solution with a pH of 3.0 to 1g of white fungus powder, place it in a hot water bath at 100°C for leaching. After 4 hours, centrifuge the leaching material at a speed of 7500rpm for 5min to remove the residue. Add 4.5 times volume of 95% ethanol solution to the supernatant, precipitate for 11 hours, centrifuge at 11,000 rpm for 6 minutes, remove the supernatant, and obtain Tremella polysaccharide precipitate.
[0038] The control test is to replace the citric acid-sodium citrate buffer solution with water as the extraction solvent, and the method is the same as above.
[0039] All the tremella polysaccharide precipitates obtained were dissolved in 20ml of water, and the polysaccharide content in the solution was measured by the phenol-sulfuric acid method to be 14.35mg / ml. The calculated polysaccharide quality in the polysac...
Embodiment 3
[0041] The commercially available white fungus is dried, crushed and passed through a 80-mesh sieve. After adding 60ml of citric acid-sodium citrate buffer solution with a pH of 3.0 to 1g of white fungus powder, place it in a hot water bath at 80°C for leaching. After 5 hours, centrifuge the leaching material at a speed of 9000rpm for 7min to remove the residue. Add four times the volume of ethanol solution with a volume concentration of 90% to the supernatant, precipitate for 10 hours, centrifuge at 10,000 rpm for 10 minutes, remove the supernatant, and obtain Tremella polysaccharide precipitate.
[0042] The control test is to replace the citric acid-sodium citrate buffer solution with water as the extraction solvent, and the method is the same as above.
[0043] All the obtained tremella polysaccharide precipitates were dissolved in 20ml of water, and the polysaccharide content in the solution was measured by the phenol-sulfuric acid method to be 5.10 mg / ml. The calculated ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More