Composition comprising photosensitive compound in polymeric nanoparticle, and method of using composition

A technology of photosensitive compounds and compositions, which can be used in drug combinations, medical preparations containing active ingredients, particle and sedimentation analysis, etc., and can solve problems such as obstruction, low photostability, and clinical application of ICG

Active Publication Date: 2018-01-19
THE STATE OF OREGON ACTING BY & THROUGH THE OREGON STATE BOARD OF HIGHER EDUCATION ON BEHALF OF OREGON STATE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low photostability in aqueous media leads to rapid photodegradation and rapid clearance from the body significantly hinders the clinical application of ICG for PDT and PTT, thereby hindering the possibility of ICG as a single theranostic in vivo

Method used

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  • Composition comprising photosensitive compound in polymeric nanoparticle, and method of using composition
  • Composition comprising photosensitive compound in polymeric nanoparticle, and method of using composition
  • Composition comprising photosensitive compound in polymeric nanoparticle, and method of using composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0160] Preparation of SiNc-PEG-PCL Nanoparticle Preparation

[0161] Silicon 2,3-naphthalocyanine bis(trihexylsilyl oxide) (SiNc, Sigma-Aldrich, St. Louis, MO) was loaded into nanoparticles to increase their water solubility and passive tumor targeting. The structural unit of the nanoparticles is methoxypoly(ethylene glycol)-b-polycaprolactone (mPEG-PCL) with a molecular weight of 15000Da.

[0162] 20mg of methoxy PEG 5000 -PCL 10000 (MW 15000) and 0.12-0.6 mg of SiNc were dissolved in 2 mL of THF. Constantly stir the SiNc-polymer solution and add 2 mL of DI H in 1 step 2 O to obtain an emulsion. The THF was removed under vacuum using a rotary evaporator, and the 2 O to adjust the final volume to 2 mL. The resulting nanosuspension was centrifuged at 10,000 rpm for 5 minutes, and the supernatant was collected and filtered using a 13 mm 0.2 μΜ nylon filter. Exemplary SiNc-NP loadings include, but are not limited to, 0.06 mg / mL derived from 0.12 mg SiNc and 20 mg polymer; ...

Embodiment 2

[0164] Size and zeta potential measurements

[0165] The hydrodynamic size and zeta potential of the prepared complexes were measured by Malvern ZetaSizer NanoSeries (Malvern Instruments, UK) according to the manufacturer's instructions. Samples were diluted with 50 mM PBS buffer (pH 7.4) to give a final SiNc concentration of approximately 1.0 μg / mL. The intensity of the He-Ne laser (633 nm) was measured at an angle of 173°. All measurements were performed at 25°C for 2 min after pre-equilibration, and each parameter was measured in triplicate. The morphology of polymer nanoparticles was observed using cryo-transmission electron microscopy (cryo-TEM). 3 μL of the nanoparticle suspension (0.3 mg / mL) was pipetted onto a glow-discharged (30 s, 30 mAmp) copper Quantifoil porous carbon support grid (Ted Pella 658-300-CU) and was analyzed using a Mark IV Vitrobot ( FEI, Hillsboro, OR) vitrified on liquid ethane. The conditions used are 100% humidity, SCPG 0 and printing time 2-4...

Embodiment 3

[0167] Absorption and Fluorescence Measurements

[0168] Absorption and fluorescence of SiNc-NPs and SiNc-NPs in saline or THF were measured using UV-1800 spectrophotometer (Shimadzu, Carlsbad, CA) and Cary Eclipse R3896 spectrofluorometer (Varian Inc., Mulgrave Victoria, Australia), respectively spectrum.

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Abstract

Embodiments of a composition comprising a photosensitive compound and a polymer nanoparticle are disclosed herein. The composition may further comprise a targeting moiety. In some embodiments, the photosensitive compound is a phthalocyanine or phthalocyanine derivative, such as a naphthalocyanine. Upon irradiation with near infrared light, the composition may be used as a fluorescent imaging agentand/or as a phototherapeutic agent, such as for photodynamic and/or photothermal therapies. In certain embodiments, the composition is used to treat certain cancers.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Patent Application No. 62 / 150,125, filed April 20, 2015, which is incorporated herein by reference in its entirety. technical field [0003] The present disclosure relates to compositions comprising a photosensitive compound and polymeric nanoparticles, and methods of using the compositions to treat diseases and conditions, including cancer. Background technique [0004] The simultaneous therapeutic and diagnostic approach known as theranostics has gained significant attention for real-time biomedical imaging and site-specific therapy of various diseases and conditions, including cancer. Phototherapy is a widely accepted non-invasive clinical method for eradicating cancer, in part because selective irradiation of cancerous sites with light of appropriate wavelengths produces minimal side effects while leaving healthy tissue unaffected. Represented by photodynamic t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/409A61K9/51A61K47/30
CPCA61K31/409A61K41/0071A61K49/0036A61K49/0093A61P1/16A61P1/18A61P13/08A61P15/00A61P17/00A61P35/00G01N15/14
Inventor 奥莱那·塔拉图拉奥莱·塔拉图拉亚当·阿拉尼
Owner THE STATE OF OREGON ACTING BY & THROUGH THE OREGON STATE BOARD OF HIGHER EDUCATION ON BEHALF OF OREGON STATE UNIV
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