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Method for preparing silkworm pupa oil for auxiliarily reducing blood lipid

A technology for assisting blood lipid lowering and silkworm chrysalis, applied in the direction of fat oil/fat refining, fat oil/fat production, fat production, etc., can solve the problems of complicated operation, affecting the quality of oil products, and high operating temperature, achieving high nutritional value and improving α- The effect of linolenic acid content

Inactive Publication Date: 2018-01-26
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hot pressing and boiling pressing have a high oil yield, but the operating temperature of machine pressing is high, and the polyunsaturated fatty acids in silkworm chrysalis oil are prone to oxidative rancidity, which affects the quality of oil products; the oil produced by raw pressing is of good quality and light in color. But the oil yield is low, and the residual oil in the meal is high
Solvent extraction method has the advantages of large output and high oil yield, but the amount of solvent used is large, the solvent residue in the product is difficult to control, and the purity of the extracted oil is not high
Supercritical CO 2 The extraction method improves the extraction rate of silkworm chrysalis oil and the content of α-linolenic acid in silkworm chrysalis oil, but the extraction equipment is expensive, the production cost is high, the operation is complicated, and the energy consumption is high, so it is not suitable for large-scale production

Method used

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  • Method for preparing silkworm pupa oil for auxiliarily reducing blood lipid
  • Method for preparing silkworm pupa oil for auxiliarily reducing blood lipid

Examples

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Effect test

Embodiment 1

[0025] A method for preparing silkworm chrysalis oil for assisting blood fat reduction, comprising the following steps:

[0026] a) Mix the silkworm chrysalis powder and water evenly according to the weight volume ratio of 6:100 to obtain a silkworm chrysalis solution with a substrate concentration of 60 mg / mL;

[0027] b) Add immobilized alkaline protease (enzyme activity: 2250U / mL) to the obtained silkworm chrysalis solution for enzymolysis at pH 7.2 and enzymolysis temperature of 48°C for 60 minutes, then inactivate the enzyme in a boiling water bath for 6 minutes, and stand to separate the liquid to obtain Crude silkworm chrysalis oil in the upper layer; wherein, the preparation method of the immobilized alkaline protease EC 3.4.21.62 comprises the following steps: adding 1.351 g FeCl to 25 mL deionized water at 25 °C 3 •6H 2 O, 0.6852g FeSO 4 •7H 2 O, then add excess ammonia water, the precipitate is obtained by centrifugation and washed with deionized water until the ...

Embodiment 2

[0030] A method for preparing silkworm chrysalis oil for assisting blood fat reduction, comprising the following steps:

[0031] a) Mix silkworm chrysalis powder and water evenly according to the weight volume ratio of 5:100 to obtain a silkworm chrysalis solution with a substrate concentration of 50 mg / mL;

[0032] b) Add immobilized alkaline protease EC 3.4.21.62 (enzyme activity: 2500 U / mL) to the obtained silkworm chrysalis solution for enzymolysis at pH 7.0 and 45°C for 70 minutes, then inactivate the enzyme in a boiling water bath for 7 minutes, Stand still and separate the liquid to obtain the crude silkworm chrysalis oil in the upper layer; wherein, the preparation method of the immobilized alkaline protease EC 3.4.21.62 comprises the following steps: at 25°C, add 1.351 g FeCl to 25 mL deionized water 3 •6H 2 O, 0.6852g FeSO 4 •7H 2 O, then add excess ammonia water, the precipitate is obtained by centrifugation and washed with deionized water until the pH is 7.0 to ...

Embodiment 3

[0035] A method for preparing silkworm chrysalis oil for assisting blood fat reduction, comprising the following steps:

[0036] a) Mix silkworm chrysalis powder and water evenly according to the weight volume ratio of 7:100 to obtain a silkworm chrysalis solution with a substrate concentration of 70 mg / mL;

[0037] b) Add immobilized alkaline protease EC 3.4.21.62 (enzyme activity: 2000U / mL) to the obtained silkworm chrysalis solution for enzymolysis at pH 7.5 and enzymolysis temperature of 50°C for 50 minutes, inactivate the enzyme in a boiling water bath for 5 minutes, and let stand Liquid separation to obtain the crude silkworm chrysalis oil in the upper layer; wherein, the preparation method of the immobilized alkaline protease EC 3.4.21.62 comprises the following steps: at 25°C, 1.351 g FeCl is added to 25 mL deionized water 3 •6H 2 O, 0.6852g FeSO 4 •7H 2 O, then add excess ammonia water, the precipitate is obtained by centrifugation and washed with deionized water u...

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Abstract

The invention discloses a method for preparing silkworm pupa oil for auxiliarily reducing blood lipid, wherein the method includes the steps: a) mixing a silkworm pupa powder and water according to the weight and volume ratio of (5-7):100 evenly, to obtain a silkworm pupa solution; b) adding the obtained silkworm pupa solution into immobilized alkaline protease EC 3.4.21.62, carrying out enzymolysis, inactivating enzymes for 5-7 min with boiling water bath, and allowing to stand for liquid separation, to obtain an upper-layer crude silkworm pupa oil; and c) carrying out degumming, deacidifyingand decoloring treatment on the obtained upper-layer crude silkworm pupa oil, to obtain the silkworm pupa oil. The specific immobilized alkaline protease EC 3.4.21.62 is adopted for enzymolysis; compared with traditional methods for extracting silkworm pupa oil, the method can significantly improve the alpha-linolenic acid content in the silkworm pupa oil, and has higher nutritional value of thesilkworm pupa oil (lower acid value, lower saponification value and higher iodine value), so that the prepared silkworm pupa oil is proved to have a significant effect for auxiliarily reducing blood lipid.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a method for preparing silkworm chrysalis oil for assisting in lowering blood fat. Background technique [0002] α-linolenic acid is an essential fatty acid for the human body, which can be converted into docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which are necessary for metabolism, and are the basic substances that constitute cell membranes and biological enzymes. It plays a decisive role in human health and can improve and prevent diseases such as hyperlipidemia, hyperglycemia and hypertension. α-linolenic acid cannot be synthesized in the human body and can only be ingested from food. At present, most of the products containing α-linolenic acid in the market are extracted and refined from perilla oil, seabuckthorn seed oil, linseed mustard oil, etc., but the extraction process is complicated, and the content of α-linolenic acid is not high. In recent years, si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B3/00
Inventor 胡腾根廖森泰邹宇晓刘凡沈维治王卫飞李倩林光月穆利霞
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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