Method for detecting acetamiprid based on nickel-ferricyanide complex nanoparticles as indication probe

A nanoparticle and indicator probe technology, applied in the direction of material electrochemical variables, can solve the problems of poor selectivity, cumbersome operation, and high price, and achieve the effects of high selectivity, simple equipment, and high sensitivity

Active Publication Date: 2018-02-02
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of the high cost and poor selectivity of the existing acetamiprid detection method and the need to label the aptamer with electroactive groups or to add Due to the technical problems caused by electroactive substances such as cumbersome operation, high price, and time-consuming, a method for the detection of acetamiprid based on nickel-ironium-ferricyanide complex nanoparticles as indicator probes is provided.

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  • Method for detecting acetamiprid based on nickel-ferricyanide complex nanoparticles as indication probe
  • Method for detecting acetamiprid based on nickel-ferricyanide complex nanoparticles as indication probe
  • Method for detecting acetamiprid based on nickel-ferricyanide complex nanoparticles as indication probe

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Embodiment 1

[0032] The acetamiprid detection method based on the nickel-iron-ferricyanide complex nanoparticles as the indicator probe in this embodiment comprises the following steps:

[0033] 1. Preparation of electrochemical aptasensors using nickel-iron-ferricyanide complex nanoparticles as indicator probes:

[0034] (1) The bare glassy carbon electrode is covered with Al 2 o 3 After powder polishing, ultrasonically clean with high-purity water, and then 2 Blow dry under atmosphere;

[0035] (2) Place the dried bare glassy carbon electrode in 0.5M H 2 SO 4 Carry out cyclic voltammetry scanning in the solution, the scanning voltage is -0.2V, and the scanning rate is 50mV / s, until the cyclic voltammetry curve reaches stability;

[0036] (3) Place the bare glassy carbon electrode treated in step (2) in a solution containing 30mM NiCl 2 ·6H 2 O and 5mM NH 4 In the aqueous solution of Cl, enrich Ni at a constant potential of -0.8V to deposit the Ni film on the surface of the bare gla...

Embodiment 2

[0045] The acetamiprid detection method based on the nickel-iron-ferricyanide complex nanoparticles as the indicator probe in this embodiment comprises the following steps:

[0046] 1. Preparation of electrochemical aptasensors using nickel-iron-ferricyanide complex nanoparticles as indicator probes:

[0047] (1) The bare glassy carbon electrode is covered with Al 2 o 3 After powder polishing, ultrasonically clean with high-purity water, and then 2 Blow dry under atmosphere;

[0048] (2) Place the dried bare glassy carbon electrode in 0.5M H 2 SO 4 Carry out cyclic voltammetry scanning in the solution, the scanning voltage is 0.5V, and the scanning rate is 70mV / s, until the cyclic voltammetry curve reaches stability;

[0049] (3) Place the bare glassy carbon electrode treated in step (2) in a solution containing 40mM NiCl 2 ·6H 2 O and 8mM NH 4 In the aqueous solution of Cl, Ni enrichment was carried out at a constant potential of -0.8V. After enrichment for 30s, the N...

Embodiment 3

[0058] The acetamiprid detection method based on the nickel-iron-ferricyanide complex nanoparticles as the indicator probe in this embodiment comprises the following steps:

[0059] 1. Preparation of an electrochemical aptasensor using nickel-ferricyanide complex nanoparticles as an indicator probe, comprising the following steps:

[0060] (1) The bare glassy carbon electrode is covered with Al 2 o 3 After powder polishing, ultrasonically clean with high-purity water, and then 2 Blow dry under atmosphere;

[0061] (2) Place the dried bare glassy carbon electrode in 0.5M H 2 SO 4 Carry out cyclic voltammetry scanning in the solution, the scanning voltage is 1.2V, and the scanning rate is 100mV / s, until the cyclic voltammetry curve reaches stability;

[0062] (3) Place the bare glassy carbon electrode treated in step (2) in a solution containing 50mM NiCl 2 ·6H 2 O and 10 mM NH 4 In the aqueous solution of Cl, Ni enrichment was carried out at a constant potential of -0.8...

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Abstract

The invention relates to a method for detecting acetamiprid based on nickel-ferricyanide complex nanoparticles as an indication probe. The problems of high cost and poor selectivity of existing acetamiprid detection methods and the problems of tedious operation, high price and long time caused by electroactive group labeling of aptamer or addition of an electroactive substance into a test system in the construction process of previous electrochemical aptamer sensors for acetamiprid are solved. The nickel-ferricyanide complex nanoparticles used as the indication probe are deposited on the surface of an electrode in situ, and a highly-sensitive electrochemical analysis technology is effectively combined with a high-affinity nuclear aptamer to construct an electrochemical aptamer sensor for acetapamidine. The method realizes highly sensitive analysis of the acetamiprid and obtaining of high selectivity in a complex environment medium, has the advantages of simple device, low analysis costand fast response, and provides a new detection method for assessing the pesticide acetamiprid residues in foods and the environment.

Description

technical field [0001] The invention relates to a method for detecting acetamiprid based on nickel-ferricyanide complex nanoparticles as indicator probes. Background technique [0002] Acetamiprid is a highly efficient, safe and long-lasting systemic chloronicotinoid pesticide. It has poisonous effects on Hemiptera, Lepidoptera, Coleoptera and other pests on fruit trees and vegetables, especially on energy Effective against organophosphate, carbamate and pyrethroid insecticides. Due to its large amount of use, it can enter the human body through the food chain. If it is eaten by mistake for a long time, it will have potential dangers to human health. Therefore, it is of great significance to seek a fast, reliable, highly sensitive and highly selective analytical method for the detection of acetamiprid residues in food and the environment. [0003] At present, the methods for detecting acetamiprid residues mainly include instrumental analysis methods such as high performanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 范丽芳张彩云郭玉晶董川
Owner SHANXI UNIV
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