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Supercritical extract of fresh Cordyceps sinensis, and preparation method and application thereof

A technology of supercritical extract and Cordyceps sinensis, applied in solvent extraction, separation method, solid solvent extraction and other directions, can solve problems such as research on unextracted substances, and achieve the effects of broad application prospects, clear active ingredients and high extraction efficiency

Inactive Publication Date: 2018-02-16
DONGGUAN HEC CORDYCEPS R&D CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing literature on supercritical CO 2 The research on fluid extraction of Cordyceps sinensis is mainly based on the artificial fermentation product of Cordyceps sinensis, Cordyceps sinensis, Cordyceps fungus powder, etc., and no in-depth research on the extract

Method used

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  • Supercritical extract of fresh Cordyceps sinensis, and preparation method and application thereof
  • Supercritical extract of fresh Cordyceps sinensis, and preparation method and application thereof
  • Supercritical extract of fresh Cordyceps sinensis, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Take fresh Cordyceps sinensis, brush away the sediment, wash, freeze-dry, crush into fine powder and pass through a 40-mesh sieve. Take 500g of crushed Cordyceps sinensis fine powder and put it into the extraction kettle, start the extraction and separation temperature control instrument, set the temperature of the extraction kettle to 32°C, and the temperature of the separation kettle to 40°C, start the refrigeration, when the refrigeration temperature reaches 6°C, open the air valve , adjust the frequency of the main pump to 18Hz, adjust the size of each valve, so that the pressure of the extraction valve is 20MPa, the pressure of the separation tank is 6MPa, and the supercritical CO 2 Circulate the extraction, every 10 minutes, open the valve of the separation kettle to collect the extract, extract for 1 hour, and obtain 30 g of yellow-brown extract, the extraction rate is 6%.

Embodiment 2

[0038] Take fresh Cordyceps sinensis, brush away the sediment, wash, freeze-dry, crush into fine powder and pass through a 40-mesh sieve. Take 500g of crushed Cordyceps sinensis fine powder and put it into the extraction kettle, start the extraction and separation temperature control instrument, set the temperature of the extraction kettle to 37°C, the temperature of the separation kettle to 40°C, start the refrigeration, when the refrigeration temperature reaches 6°C, open the air valve , adjust the frequency of the main pump to 22Hz, adjust the size of each valve, so that the pressure of the extraction valve is 25MPa, the pressure of the separation tank is 5MPa, and the supercritical CO 2 Circulate the extraction, every 10 minutes, open the valve of the separation kettle to collect the extract, extract for 1.5 hours, and obtain 37 g of yellow-brown extract, the extraction rate is 7.4%.

Embodiment 3

[0040] Take fresh Cordyceps sinensis, brush away the sediment, wash, freeze-dry, crush into fine powder and pass through a 40-mesh sieve. Take 500g of crushed Cordyceps sinensis fine powder and put it into the extraction kettle, start the extraction and separation temperature control instrument, set the temperature of the extraction kettle to 35°C, the temperature of the separation kettle to 45°C, start the refrigeration, when the refrigeration temperature reaches 6°C, open the air valve , adjust the frequency of the main pump to 20Hz, adjust the size of each valve, so that the pressure of the extraction valve is 23MPa, the pressure of the separation tank is 4MPa, and the supercritical CO 2 Circulate the extraction, every 10 minutes, open the valve of the separation kettle to collect the extract, extract for 2 hours, and obtain 34 g of yellow-brown extract, the extraction rate is 6.8%.

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Abstract

The invention relates to a supercritical extract, specifically to a supercritical extract of fresh Cordyceps sinensis and a preparation method thereof, and application of the supercritical extract topreparation of drugs for treating lung cancer. The preparation method for the supercritical extract comprises the following steps: subjecting fresh Cordyceps sinensis to freeze drying, then carrying out crushing and carrying out sieving with a 40-mesh sieve; filling an extraction kettle with the obtained fine powder of Cordyceps sinensis, introducing a supercritical CO2 fluid, setting extraction parameters and carrying out extraction for 1 to 2 h; and collecting an extraction product so as to obtain the supercritical extract of fresh Cordyceps sinensis. The extract provided by the invention isprepared from fresh Cordyceps sinensis through supercritical CO2 fluid extraction, and the obtained extract contains nucleosides, aliphatic acids and sterols and has obvious inhibitory effect on thehuman non-small cell lung cancer A549; the extract is clear in active ingredients and has good application prospects; and the preparation method is free of toxicity and residues during preparation, high in security, simple to operate, high in extraction yield and easy to realize large-scale production.

Description

technical field [0001] The invention relates to a supercritical extract, in particular to a supercritical extract of fresh Cordyceps sinensis, a preparation method of the extract and an application in the field of preparation of drugs for treating lung cancer. Background technique [0002] Cordyceps sinensis is a compound of the subunit and larva corpse of Cordyceps sinensis (BerK.) Sacc, a fungus of the family Ergotaceae, which parasitizes on the larvae of bat moths. The three major supplements. Chinese medicine believes that Cordyceps sinensis can nourish the lungs and kidneys, relieve cough and reduce phlegm, and is used for kidney essence deficiency, impotence and nocturnal emission, soreness of the waist and knees, chronic cough and shortness of breath, consumptive cough and hemoptysis ("Chinese Pharmacopoeia" 2015 edition). Modern pharmacological studies have shown that Cordyceps sinensis has the effects of lowering blood sugar, lowering blood fat, anti-inflammation, ...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P35/00B01D11/02
CPCA61K36/068A61K2236/37B01D11/0203
Inventor 田野李周钱正明李文佳温宝庆肖瑛张蓉
Owner DONGGUAN HEC CORDYCEPS R&D CO LTD
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