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Heparin sodium detection through proportional fluorescence method based on carbon-quantum-dot-quenched fluorescence-labeled DNA

A technology of carbon quantum dots and fluorescent labeling, which is applied in the field of detection of heparin sodium, can solve problems such as ratiometric fluorescence analysis methods for the determination of heparin, and achieve the effects of high sensitivity, convenient operation, and simple measurement conditions

Active Publication Date: 2018-02-16
FUJIAN MEDICAL UNIV
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Problems solved by technology

It is reported that gold nanoparticles can be used for the determination of heparin, but there is still no report on the determination of heparin by ratiometric fluorescence analysis

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  • Heparin sodium detection through proportional fluorescence method based on carbon-quantum-dot-quenched fluorescence-labeled DNA
  • Heparin sodium detection through proportional fluorescence method based on carbon-quantum-dot-quenched fluorescence-labeled DNA
  • Heparin sodium detection through proportional fluorescence method based on carbon-quantum-dot-quenched fluorescence-labeled DNA

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Embodiment Construction

[0026] (1) The present invention will be described in detail below in conjunction with specific embodiments and accompanying drawings. It should be understood that the specific embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0027] (2) Example (feasibility analysis)

[0028] The sample of the feasibility experiment of this embodiment is made through the following steps:

[0029] Prepare the following solutions: solution 1 is CQDs; solution 2 is FAM-ssDNA; solution 3 is CQDs+FAM-ssDNA; solution 4 is CQDs, FAM-ssDNA and sodium heparin. Among them, the concentration of CQDs is 6.25 μg / mL, the concentration of FAM-ssDNA is 50 nM, and the concentration of sodium heparin is 2.0 μg / mL. The three component solutions are all prepared with phosphate buffer solution. The buffer solution used in the embodiment of the present invention (buffer solution is composed of Na 2 HPO 4 ﹒ 12H 2 O,...

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Abstract

The invention discloses heparin sodium detection through a proportional fluorescence method based on carbon-quantum-dot-quenched fluorescence-labeled DNA. Heparin sodium can recover the fluorescence of carbon-quantum-dot-quenched FAM-labeled DNA, with the concentration rise of heparin sodium, recovery of FAM is improved, and the original fluorescence intensity of carbon quantum dots is kept stable, thereby establishing a new ratio-type fluorescence method for heparin sodium detection. The heparin sodium detection method is simple in steps, and heparin sodium and DNA fluorescence recovery showgood linear correlation. The linear correlation is low and can reach 9.04 ng / mL. In fact, the recovery of heparin sodium in a rabbit serum sample is in a range of 95.47-111.95%. A fitted curve for simulating detection of heparin sodium in serum is close to the standard curve. Therefore, a serum sample doesn't interfere in a detection result. Compared with other heparin sodium detection methods, the method is excellent in detection performance and simple to operate. A new method selection is provided for clinical heparin sodium detection.

Description

technical field [0001] The invention relates to the application field of carbon nanomaterials, in particular to a proportional fluorescence method for detecting heparin sodium based on carbon quantum dots quenching FAM-labeled ssDNA. Background technique [0002] (1) Heparin (Hep for short) is a mucopolysaccharide sulfate composed of glucosamine, L-iduroside, N-acetylglucosamine and D-glucuronic acid alternately, with a relative average molecular weight of 15 KD, It is highly acidic and has a strong negative charge. Heparin sodium can slow or reduce blood coagulation and is a very effective anticoagulant drug. In hospitals, heparin is used to prevent blood clots and is a common anticoagulant drug for patients requiring hemodialysis. However, for different diseases, heparin will have different optimal doses. This is because the main side effects of heparin are spontaneous bleeding and thrombocytopenia. Great pain may also cause medical disputes. Therefore, clinically, it i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432G01N2021/6439
Inventor 翁少煌林新华李凤兰郭茹彬赵东
Owner FUJIAN MEDICAL UNIV
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