Separation method for silk fibroin peptides with small molecular weights

Abstract

The invention provides a separation method for silk fibroin peptides with small molecular weights. The separation method comprises the steps: degumming and dissolving silkworm silk to obtain a solution; adding the solution into a glucan G10 column, carrying out elution by using sterile water, and carrying out collection to obtain a desalted silk fibroin protein aqueous solution; freeze-drying thedesalted silk fibroin protein aqueous solution to obtain freeze-dried silk fibroin proteins; adding a freeze-dried silk fibroin protein solution into a glucan G50 column, eluting the silk fibroin proteins by using the sterile water, and collecting the silk fibroin proteins by using different tubes; screening the silk fibroin peptides of which the molecular weights are smaller than 1500Da in collecting tubes in the step (d), adding the silk fibroin peptides into a glucan G15 column again, eluding the silk fibroin peptides by using the sterile water, collecting the silk fibroin peptides by usingdifferent collecting tubes, and carrying out separation to obtain multilevel silk fibroin peptides with small molecular weights being 1500Da or smaller. The multilevel silk fibroin peptides with small molecular weights being 1500Da or smaller are obtained through dissolution with neutral salts and gradient separation of different molecular sieve columns. Meanwhile, the hydrophilicity of the silkfibroin peptides is made better by using a specific freeze-drying-dissolving method.

Description

technical field [0001] The invention relates to the technical field of textiles, in particular to a method for separating silk fibroin with small molecular weight. Background technique [0002] Bombyx mori silk is a natural animal protein synthesized and secreted by silkworms. It has a wide range of sources. Its silk fibroin has good biocompatibility and is composed of 20 kinds of amino acids that can be absorbed by the human body. The final degradation products are amino acids or small peptides, which are easily absorbed by cells. Absorption or phagocytosis will not cause obvious immune response. A large number of researches at home and abroad have shown that silk fibroin materials can support the growth of various cells, so silk fibroin is an ideal raw material for the development of skin care products. However, as a skin care product, whether it can be absorbed by the skin is very important, which has certain requirements for the molecular weight of silk protein. Before ...

AI Technical Summary

Problems solved by technology

The traditional acid or alkali dissolution method can obtain silk fibroin with small molecular weight. This method cannot control the dissolution of silk fibroin. Although compared with the salt dissolution method to obtain a small molecular weight, it will destroy some amino acids when the silk fiber is dissolved. , greatly affect the biological activity of silk fibroin molecules

The above reported silk fibroin solutions obtained by solution dialysis, membrane filtration or acid-base dissolution are mixed with biological extracts, which are used in skin care products or food with peculiar smell