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Method for extracting yellow pigment from Malus toringoides

A technology of changing leaf crabapple and yellow pigment, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of application limitations, no better method for extracting yellow pigment, asthma and rubella

Inactive Publication Date: 2018-02-23
炉霍雪域俄色有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Yellow pigment is a kind of pigment that is very much needed in the food and cosmetic industry. In the past, it was usually replaced by artificial tartrazine, but this synthetic pigment is easy to cause people to suffer from asthma and rubella, so it has been banned in many countries
However, it has always been a difficult problem to find natural water-soluble yellow pigments, and the few natural yellow pigments are greatly limited in application due to various defects.
[0003] At present, there is a method for extracting yellow pigment from apples, but there is no better method for extracting yellow pigment from Begonia variegata, a plant that grows in high-altitude areas.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0024] Step 1: drying the fibrous slag of crabapple after squeezing the juice;

[0025] Step 2: The dried fiber slag is lowered to room temperature and crushed repeatedly until completely sieved;

[0026] Step 3: Mix the pulverized fiber slag with ethanol and chloroform solution, the ratio of solid to liquid is 1:10, the volume ratio of ethanol and chloroform is 5:1, the extraction temperature is 60°C-80°C, and the extraction time is 100min to repeat the extraction , filter, leave and separate, obtain supernatant;

[0027] Step 4: Use KOH to adjust the pH value of the supernatant to between 4 and the KOH concentration to be 2%, and use the macroporous adsorption resin to absorb the active ingredient phlorizin in the solution;

[0028] Step 5: eluting, concentrating, and drying with ethanol with a volume concentration greater than 90%;

[0029] Step 6: put phloridzin, glucosidase, and glucosidase in acetic acid-sodium acetate buffer solution (PH), pass through oxygen to stir,...

specific Embodiment 2

[0032] Step 1: drying the fibrous slag of crabapple after squeezing the juice;

[0033] Step 2: The dried fiber slag is lowered to room temperature and crushed repeatedly until completely sieved;

[0034] Step 3: Mix the pulverized fiber slag with ethanol and chloroform solution, the ratio of solid to liquid is 1:50, the volume ratio of ethanol and chloroform is 5:1, the extraction temperature is 60°C-80°C, and the extraction time is 200min to repeat the extraction , filter, leave and separate, obtain supernatant;

[0035] Step 4: Use KOH to adjust the pH value of the supernatant to between 5 and the KOH concentration to be 5%, and use the macroporous adsorption resin to absorb the active ingredient phlorizin in the solution;

[0036] Step five: eluting with absolute ethanol, concentrating, and drying;

[0037] Step 6: put phloridzin, glucosidase, and glucosidase in acetic acid-sodium acetate buffer solution (PH), pass through oxygen to stir, and carry out enzymatic conversi...

specific Embodiment 3

[0040] Step 1: drying the fibrous slag of crabapple after squeezing the juice;

[0041] Step 2: The dried fiber slag is lowered to room temperature and crushed repeatedly until completely sieved;

[0042] Step 3: Mix the crushed fiber slag with ethanol and chloroform solution, the solid-liquid ratio is 1:20, the volume ratio of ethanol and chloroform is 5:1, the extraction temperature is 60°C-80°C, and the extraction time is 50min-200min Repeat Leaching, filtering, leaving and separating to obtain the supernatant;

[0043] Step 4: Use KOH to adjust the pH value of the supernatant to between 5 and the KOH concentration to be 3%, and use the macroporous adsorption resin to absorb the active ingredient phlorizin in the solution;

[0044] Step 5: eluting, concentrating, and drying with ethanol with a volume concentration greater than 90%;

[0045] Step 6: put phloridzin, glucosidase, and glucosidase in acetic acid-sodium acetate buffer solution (PH), pass through oxygen to stir,...

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PUM

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Abstract

The present invention discloses a method for extracting yellow pigment from Malus toringoides. The method comprises: 1, drying juiced Malus toringoides fiber residue; 2, cooling the dried fiber residue to a room temperature, and repeatedly crushing until completely screening; 3, mixing the crushed fiber residue, ethanol and a chloroform solution, repeatedly leaching and filtering, and separating to obtain a supernatant; 4, adjusting the PH value of the supernatant to 4-5 with KOH, and adsorbing the effective component phlorizin in the solution by using an adsorption resin; 5, carrying out elution with ethanol, concentrating, and drying to obtain phlorizin; 6, adding the phlorizin, glucosidase and polyphenol oxidase to an acetic acid-sodium acetate buffer solution, introducing oxygen, stirring, carrying out an enzyme catalysis conversion reaction, heating to inactivate the enzyme after completing the reaction, extracting with ethanol, concentrating the lower layer aqueous phase, and drying; 7, dissolving the dried crude product in methanol, removing the buffer salt, concentrating the supernatant, and drying to obtain the yellow pigment fine produt.

Description

technical field [0001] The invention relates to the extraction of effective plant components, in particular to a method for extracting phloridzin from crabapple slag. Background technique [0002] Yellow pigment is a kind of pigment that is very much needed in the food and cosmetic industry. In the past, it was usually replaced by artificial tartrazine. However, this synthetic pigment is easy to cause asthma and rubella, so it has been banned in many countries. However, it has always been a difficult problem to find natural water-soluble yellow pigments, and the few natural yellow pigments are greatly limited in application due to various defects. [0003] At present, there is a method for extracting yellow pigment from apples, but there is no better method for extracting yellow pigment from Begonia variegata, a plant that grows at high altitudes. Contents of the invention [0004] The object of the present invention is to: aim at the above-mentioned problem, provide a ki...

Claims

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Application Information

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IPC IPC(8): C09B61/00
CPCC09B61/00
Inventor 雷敏谢雨蕉土布毛羽
Owner 炉霍雪域俄色有限责任公司
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