Preparation method of double-emission fluorescent probes for detecting content of aspirin in saliva

A dual-emission fluorescence and aspirin technology is applied in the field of preparation of dual-emission ratiometric fluorescent probes, which can solve the problems of complex sample processing, long time-consuming, and expensive detection costs, and achieve short process, stable structure and composition. Apply quick and easy effects

Inactive Publication Date: 2018-02-23
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these instrumental detection methods require complex sample processing, high detection costs, and time-consuming

Method used

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  • Preparation method of double-emission fluorescent probes for detecting content of aspirin in saliva
  • Preparation method of double-emission fluorescent probes for detecting content of aspirin in saliva
  • Preparation method of double-emission fluorescent probes for detecting content of aspirin in saliva

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Dissolve 0.5mmol tellurium powder in aqueous solution of sodium hydride tellurium, and add it to 1.0mmol cadmium chloride (CdCl 2 ) and 2.4 mmol thioglycolic acid (TGA) mixed solution, reflux at 100 °C for 2 hours and 24 hours, respectively, to prepare CdTe nanocrystals with green light emission (g-QDs) and red light emission (r-QDs).

[0032] (2) Mix 10 mL r-QDs solution and 40 mL ethanol solution in a conical flask, add 50 μL 3-aminopropyltriethoxysilane (APTES), and stir for 5 hours. Subsequently, 1.2 mL of tetraethoxysilane (TEOS) and ammonia water were added, stirring was continued for 12 hours, and 100 μL of 3-aminopropyltriethoxysilane (APTES) was added thereto. After the reaction was completed, it was centrifuged and washed several times to obtain r-QDs@SiO embedded in silicon coating. 2 Material.

[0033] (3) Take 100μL r-QDs@SiO 2 Disperse with g-QDs in 12mg tris(hydroxymethyl)aminomethane buffer, then add 40μL 1-ethyl-3-(3-dimethylaminopropyl)carbodiim...

Embodiment 2

[0040] (1) Dissolve 0.5mmol tellurium powder in aqueous solution of sodium hydride tellurium, and add it to 1.0mmol cadmium chloride (CdCl 2 ) and 2.4mmol thioglycolic acid (TGA), reflux at 100°C for 3 hours and 22 hours, respectively, to prepare CdTe nanocrystals with green light emission (g-QDs) and red light emission (r-QDs).

[0041] (2) Mix 10 mL r-QDs solution and 40 mL ethanol solution in a conical flask, add 50 μL 3-aminopropyltriethoxysilane (APTES), and stir for 5 hours. Subsequently, 1.2 mL of tetraethoxysilane (TEOS) and ammonia water were added, stirring was continued for 12 hours, and 100 μL of 3-aminopropyltriethoxysilane (APTES) was added thereto. After the reaction was completed, it was centrifuged and washed several times to obtain r-QDs@SiO embedded in silicon coating. 2 Material.

[0042] (3) Take 100μL r-QDs@SiO 2 Disperse with g-QDs in 12mg tris(hydroxymethyl)aminomethane buffer, then add 40μL 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 36mg N-hy...

Embodiment 3

[0044] (1) Dissolve 0.5mmol tellurium powder in NaHTe aqueous solution, and add it to 1.0mmol cadmium chloride (CdCl 2 ) and 2.4mmol thioglycolic acid (TGA), reflux at 100°C for 4 hours and 20 hours, respectively, to prepare CdTe nanocrystals with green light emission (g-QDs) and red light emission (r-QDs).

[0045] (2) Mix 10 mL r-QDs solution and 40 mL ethanol solution in a conical flask, add 50 μL 3-aminopropyltriethoxysilane (APTES), and stir for 5 hours. Subsequently, 1.2 mL of tetraethoxysilane (TEOS) and ammonia water were added, stirring was continued for 12 hours, and 100 μL of 3-aminopropyltriethoxysilane (APTES) was added thereto. After the reaction was completed, it was centrifuged and washed several times to obtain r-QDs@SiO embedded in silicon coating. 2 Material.

[0046] (3) Take 100μL r-QDs@SiO 2 Disperse with g-QDs in 12mg tris(hydroxymethyl)aminomethane buffer, then add 40μL 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 36mg N-hydroxysuccinyl The mix...

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Abstract

The invention discloses a preparation method of double-emission fluorescent probes for detecting the content of aspirin in saliva, and belongs to the technical field of human health inspection. The preparation method comprises the following steps of preparation of cadmium telluride quantum dot probes with different luminescent colors, preparation of red-luminescent cadmium telluride quantum dot probes coated with inorganic silicon coating, and preparation of double-emission ratio-type fluorescent probes. The prepared fluorescent probes are simple and efficient, convenient and high in accuracy,can remove data distortion caused by photobleaching, probe load and remaining, device illuminance stability and the like, and can be identified and judged with naked eyes and have longer service life.

Description

technical field [0001] The invention belongs to the technical field of human health inspection, and the technology relates to a preparation method of a double emission ratio fluorescent probe for detecting the content of aspirin in human saliva. Background technique [0002] As an analgesic, antipyretic, and anti-inflammatory drug that has been used for hundreds of years, aspirin is widely used to treat headaches, joint pains, toothaches, rheumatism, fever, colds, etc., and is also used to prevent and treat cardiovascular diseases , thrombosis, gastrointestinal tumors, etc. However, long-term use of aspirin in large quantities by the human body will cause many dysfunctions such as gastric mucosal damage, liver damage, and kidney damage. When the blood concentration of the drug is high (>200μg / mL), there will be obvious side effects, such as nausea, vomiting, upper abdominal discomfort, etc., and even gastrointestinal bleeding or ulcers. Therefore, it is particularly imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/88G01N21/64
CPCC09K11/02C09K11/025C09K11/883G01N21/6428G01N21/6447
Inventor 陈厉孙琳郑佳红戴江栋吴易霖戴晓晖李春香闫永胜
Owner JIANGSU UNIV
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