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Tuna fish bone gelatin antifreeze peptide and preparation method thereof

A technology of fish bone gelatin and tuna, applied in chemical instruments and methods, specific peptides, connective tissue peptides, etc., can solve the problems of complex production process of natural antifreeze, unsuitable for industrial production, time-consuming and costly, and achieve good market prospects , Inhibition of ice recrystallization, and the effect of reducing production costs

Inactive Publication Date: 2018-03-02
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current production process of natural antifreeze is relatively complicated and has low activity, which is time-consuming and expensive, and is not suitable for industrial production.

Method used

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  • Tuna fish bone gelatin antifreeze peptide and preparation method thereof
  • Tuna fish bone gelatin antifreeze peptide and preparation method thereof
  • Tuna fish bone gelatin antifreeze peptide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A preparation method of tuna fish bone gelatin antifreeze peptide, the preparation process is as follows: tuna fish bone "removal of non-collagen and decalcification "double enzyme enzymatic hydrolysis" enzymatic hydrolyzate "ultrafiltration" macroporous resin purification "cation exchange layer Analysis of "gel filtration chromatography" antifreeze peptides.

[0024] The specific steps are:

[0025] (1) Preparation of tuna bone gelatin: bonito ( Bullet mackerel ) Fish bones were decalcified with 0.1 mol / L NaOH solution and decalcified with 0.5 mol / L EDTA solution, then 1.0 mol / L HCl solution was added to the decalcified fish bone powder according to the solid-liquid ratio of 1 g:13 mL to treat After 17 h, wash the fish bone powder with water until neutral, then add distilled water to the fish bone powder according to the ratio of solid to liquid 1 g:14 mL, extract at 65 °C for 9 h, filter, concentrate the filtrate in vacuum, and dry at 65 °C to obtain fish bone powde...

Embodiment 2

[0033] A preparation method of tuna fish bone gelatin antifreeze peptide, the optimization steps are:

[0034] (1) Preparation of tuna bone gelatin: bonito ( Bullet mackerel ) Fish bones were decalcified with 0.1 mol / L NaOH solution and decalcified with 0.5 mol / L EDTA solution, then 1.0 mol / L HCl solution was added to the decalcified fish bone powder according to the solid-liquid ratio of 1 g:15 mL, and treated After 16 hours, wash the fishbone powder with water until neutral, then add distilled water to the fishbone powder according to the ratio of material to liquid: 1 g: 15mL, extract at 65°C for 9 hours, filter, concentrate the filtrate in vacuum, and dry it in vacuum at 65°C to obtain fishbone gelatin ;

[0035] (2) Enzymatic hydrolysis of tuna fish bone gelatin: add fish bone gelatin to double distilled water at a solid-to-liquid ratio of 1g:14 mL, adjust the pH of the solution to 3.0 with HCl, heat the solution to 40°C, and add 1.5% gelatin mass to gelatin Add pepsin...

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Abstract

The invention discloses tuna fish bone gelatin antifreeze peptide and a preparation method thereof. The antifreeze peptide, with tuna fish bones as a raw material, is prepared by conducting non-collagen protein removal and decalcification, and conducting bi-enzyme enzymolysis so as to obtain enzymatic hydrolysate; and separating and purifying the enzymatic hydrolysate through ultrafiltration, macroporous resin purification, cation exchange resin chromatography and gel column chromatography. Based upon detection and analysis, the average molecular weight of the antifreeze peptide is 1250.0-1315.0 Da; and the antifreeze peptide mainly consists of four polypeptides, namely GPAGQEGPAGLVG, GFRGPAGTIGLVG, GFIGPAGPAG and GPSGPLGFPGPSG, at the mass ratio of 1 to (1.78-1.93) to (0.85-0.91) to (0.73-0.84). Based upon an escherichia coli activity protection experiment, it is indicated that the tuna fish bone gelatin antifreeze peptide can take a relatively good protective effect on escherichia coli at low temperature.

Description

technical field [0001] The invention belongs to the technical field of active peptide preparation, in particular to a tuna fish bone gelatin antifreeze peptide and a preparation method thereof. Background technique [0002] Tuna belongs to Perciformes Scombroids, also known as Tuna, also known as "Tuna (fish)" in the Chinese world, is a warm-water migratory fish in the ocean, mainly distributed in the Indian Ocean, the central Pacific Ocean and the Central Atlantic Ocean. Welcome seafood with high economic value. The flesh color of tuna is red, and this is because a large amount of myoglobin is contained in the muscle of tuna. Tuna swims a long distance, and tuna swimming from California, USA have been found off the coast of Japan in the past. There are 8 varieties of tuna, most of which are huge in size, with the largest body reaching 3.5 meters and weighing 600 to 700 kilograms, while the smallest variety only weighs 3 kilograms. The reproduction ability of tuna is very...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/18C07K1/16
CPCC07K14/78C12P21/06
Inventor 郁迪王斌陶静赵玉勤
Owner ZHEJIANG OCEAN UNIV
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