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A pretreatment method suitable for detection of heavy metal ions in soil by ELISA

A technology of heavy metal ions and enzyme-linked immunoassay, which is applied in the field of pretreatment of heavy metal ions in soil by enzyme-linked immunoassay, can solve the problems of high price, long analysis time, and high analysis cost, so as to avoid false negative results and shorten the detection time. The effect of time and simple operation process

Active Publication Date: 2021-02-05
广东环美机电检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the traditional heavy metal detection methods have the advantages of high accuracy, high sensitivity, and good selectivity, but there are also many shortcomings: for example, most methods require large-scale instruments, which are expensive and costly to analyze, and some samples require complex pre-treatment processes , long analysis time, etc., unable to carry out continuous monitoring and on-site determination of heavy metals
In recent years, as the problem of heavy metal pollution has become increasingly prominent, traditional heavy metal detection methods have been difficult to meet people's requirements, and heavy metal detection methods that are simple, fast, efficient and suitable for on-site operations have become a research hotspot.

Method used

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  • A pretreatment method suitable for detection of heavy metal ions in soil by ELISA
  • A pretreatment method suitable for detection of heavy metal ions in soil by ELISA
  • A pretreatment method suitable for detection of heavy metal ions in soil by ELISA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Collection and preparation of soil samples

[0053] In June 2015, soil sampling was carried out near the Academy of Sciences of Jinan University. Soil sampling methods and procedures refer to the principles and methods in "Technical Specifications for Soil Environmental Monitoring" (HJ / T 166-2004). The sampling sites are all 0-20cm topsoil, and the snake sampling method is used. The soil from 10 sampling points is mixed into one soil sample, the stones and animal and plant residues in the soil sample are removed, and about 1kg is taken by the quartering method. Soil in teflon sealed bags. Dry the soil sample in a blast drying oven at 90°C, take about 0.25kg of soil by the quartering method, crush it with an agate rod, pass through a 2mm nylon sieve, mix well, continue to grind, and pass through 0.154mm (100 mesh) Check the sieve and store in a cool, dry place for later use.

[0054] 2. Determination of physical and chemical properties of standard soil and soil sam...

Embodiment 2 3

[0116] Embodiment 2 The adsorption and elution of aluminum tripolyphosphate to heavy metal ions

[0117] 1. Pretreatment of aluminum tripolyphosphate

[0118] The aluminum tripolyphosphate was soaked in 5M nitric acid for 30min before use, then centrifuged at 4000rpm for 10min, the supernatant was removed, washed with secondary water until the supernatant was washed to neutrality, the precipitate was filtered with suction, and then Dry at 100°C, grind and sieve, and store in a dry place protected from light for later use.

[0119] 2. Adsorption and elution of heavy metal ions by aluminum tripolyphosphate

[0120] 1) Test steps

[0121] (A) The effect of pH on the adsorption rate of aluminum tripolyphosphate

[0122] Add 2mL of 1μg·mL to a 50mL colorimetric tube with a stopper -1 cadmium ion standard solution, then add 40mL of dilute acid leaching simulated solution (i.e. take 100mL of 4M hydrochloric acid, 300mL of 4M sulfuric acid, dilute to 1L with ultrapure water), mix ...

Embodiment 3 2

[0153] Example 3 Adsorption and elution of heavy metal ions by titanium dioxide

[0154] 1. Pretreatment of titanium dioxide

[0155] Titanium dioxide (anatase, hydrophilic and lipophilic type, 5-10nm, purity 99.8%, Guangzhou Chemical Reagent Factory) was soaked in 5M nitric acid for 30min before use, then centrifuged at 4000rpm for 10min, removed the supernatant, and carried out with secondary water. Wash until the supernatant is washed to neutrality, filter the precipitate with suction, then dry at 100° C., grind and sieve, and store in a dry place protected from light for future use.

[0156] 2. Nano TiO 2 Adsorption and elution of heavy metal ions

[0157] 1) Test steps

[0158] (A) pH on nano-TiO 2 Effect of adsorption rate

[0159] Add 2mL of 1μg·mL to a 50mL colorimetric tube with a stopper -1 cadmium ion standard solution, then add 40mL of dilute acid leaching simulated solution (i.e. take 100mL of 4M hydrochloric acid, 300mL of 4M sulfuric acid, dilute to 1L wit...

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Abstract

The invention discloses a pretreatment method suitable for detecting heavy metal ions in soil by enzyme-linked immunoassay. The present invention extracts soil with dilute acid, removes insoluble substances, and obtains solution I; adjusts the pH value of solution I with ammonia water, obtains solution II; carries out the following treatment to solution II according to the soil quality grade: when the soil is divided according to the soil environment quality standard , when it is a third-grade soil, add disodium EDTA to solution III for chelation to obtain a solution that can be detected with colloidal gold test paper; when the soil is classified according to the soil environmental quality standard and is soil below the second grade, add The adsorbent is used for adsorption, solid-liquid separation, and disodium EDTA solution is added to the solid for desorption to obtain a solution that can be detected by colloidal gold test paper. The invention can carry out pretreatment on various soils in all directions, combined with colloidal gold test paper for detection, and can complete the determination of heavy metal ions (such as cadmium ions) in soil samples within 2 hours, which greatly shortens the detection time and does not require large instruments With professional experimenters, the operation process is simple, and it is an efficient, fast and simple heavy metal detection method.

Description

technical field [0001] The invention belongs to the field of soil heavy metal detection, in particular to a pretreatment method suitable for enzyme-linked immunosorbent assay to detect heavy metal ions in soil. Background technique [0002] Soil is the material condition for human survival and a precious natural resource for human beings. In recent years, with the acceleration of urbanization and industrialization in my country, coupled with the mass production and use of chemical fertilizers and pesticides, soil pollution in my country has become more and more serious, especially heavy metal pollution. This poses a potential threat to environmental ecology and people's life safety. [0003] The premise of soil remediation is to understand the status of soil heavy metal pollution. Most of the traditional heavy metal detection methods have the advantages of high accuracy, high sensitivity and good selectivity, but they also have many disadvantages: for example, most methods...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30G01N33/579
CPCG01N1/28G01N1/30G01N33/579
Inventor 江天久高东辉唐勇
Owner 广东环美机电检测技术有限公司