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Preparation method of lophiiformes bone collagen peptide and active calcium containing health-care oral liquid

A technology of collagen peptide and anglerfish, which is applied in food science and other directions, can solve problems such as being in the blank stage, and achieve the effects of shortening enzymatic hydrolysis time, simple production process and easy digestion.

Inactive Publication Date: 2018-03-06
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, using anglerfish bones as materials, using enzymatic technology, ultrasonic-microwave combined to assist in extracting collagen peptides from anglerfish bones, and using fish bones to prepare active calcium is still in a blank stage

Method used

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  • Preparation method of lophiiformes bone collagen peptide and active calcium containing health-care oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038](1) Anglerfish bones are cleaned, crushed through a 60-mesh sieve, and then soaked in 0.2mol / L NaOH solution at 5°C for 3 hours according to the material-liquid ratio of 1g:20ml to remove impurities; after repeated washing and draining, according to the material-liquid ratio of 1g: Soak 15ml of 10% propanol solution at 5°C for 12 hours to remove fat, and dry to obtain defatted fish bone powder;

[0039] (2) Add 0.2 mol / L boric acid buffer solution to the defatted fish bone powder obtained in step (1) until the defatted fish bone powder is submerged, and at a temperature of 40°C, add subtilisin for enzymatic hydrolysis for 12 minutes, the defatted fish bone powder The mass ratio of bone powder to subtilisin is 100:1, and the enzyme activity of said subtilisin is 800u / mg;

[0040] (3) Adjust the temperature of the enzymolysis solution obtained in step (2) to 45°C, adjust the pH to 9.0-10.0, add alkaline protease, and perform enzymolysis for 15 minutes; the mass ratio of th...

Embodiment 2

[0048] (1) Anglerfish bones are cleaned, crushed through a 70-mesh sieve, and then soaked at 5°C for 2 hours to remove impurities according to the material-liquid ratio of 1g:10ml and 0.2mol / L NaOH; after repeated washing and draining, according to the material-liquid ratio of 1g: Add 10% propanol to 18ml, soak at 5°C for 12 hours to remove fat, and dry to obtain defatted fish bone meal;

[0049] (2) Add 0.2 mol / L boric acid buffer solution to the defatted fish bone powder obtained in step (1) until the defatted fish bone powder is submerged, and at a temperature of 45°C, add subtilisin for enzymatic hydrolysis for 15 minutes, the defatted fish bone powder The mass ratio of bone powder to subtilisin is 100:1.3, and the enzyme activity of said subtilisin is 800u / mg;

[0050] (3) Adjust the temperature of the enzymolysis solution obtained in step (2) to 45° C., adjust the pH to 9.5, add alkaline protease, and carry out enzymolysis for 15 minutes; the mass ratio of the defatted f...

Embodiment 3

[0058] (1) Anglerfish bones are cleaned, crushed through a 70-mesh sieve, and then soaked at 5°C for 2 hours to remove impurities according to the material-liquid ratio of 1g:10ml and 0.2mol / L NaOH; after repeated washing and draining, according to the material-liquid ratio of 1g: Add 10% propanol to 18ml, soak at 5°C for 12 hours to remove fat, and dry to obtain defatted fish bone meal;

[0059] (2) Add 0.2 mol / L boric acid buffer solution to the defatted fish bone powder obtained in step (1) until the defatted fish bone powder is submerged, and at a temperature of 50°C, add subtilisin for enzymolysis for 10 minutes, and the defatted fish bone powder The mass ratio of bone meal to subtilisin is 100:1.5, and the enzyme activity of said subtilisin is 800u / mg;

[0060] (3) Adjust the temperature of the enzymolysis solution obtained in step (2) to 55° C., adjust the pH to 10, add alkaline protease, and carry out enzymolysis for 15 minutes; the mass ratio of the defatted fish bone...

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Abstract

The invention discloses a preparation method of an anglerfish bone collagen peptide and active calcium health-care oral liquid. The anglerfish bone is cleaned, crushed and sieved, then added in NaOH solution, soaked to remove miscellaneous proteins; after repeated washing and draining, adding acrylic acid Alcohol solution, soaked to remove fat, and dried to obtain defatted fish bone powder; Enzymolysis was carried out by endopeptidase, and combined with ultrasonic-microwave assisted enzymolysis; the obtained enzymolysis solution was heated, inactivated, centrifuged, and the supernatant was concentrated. The anglerfish bone collagen peptide concentrate is obtained; the remaining fish bone powder is dried, added with lactic acid, activated, adjusted for pH, filtered, heated in a water bath, and concentrated to obtain the active calcium lactate concentrate. The preparation method of the invention is simple, scientific and reasonable, utilizes raw materials to the greatest extent, and is environmentally friendly and economical.

Description

technical field [0001] The invention relates to the technical field of fish processing, in particular to a method for preparing an anglerfish bone collagen peptide and active calcium health-care oral liquid. Background technique [0002] Anglerfish is a cold-temperate bottom fish widely distributed in the eastern waters of my country (the northern part of the East China Sea, the Yellow Sea and the Bohai Sea). Fish roe is a high-nutrition food, the skin can be used to make glue, and the liver can be used for cod liver oil. Regular consumption of anglerfish liver can help protect eyesight and prevent liver diseases. Therefore, at present, anglerfish mainly process fish meat and liver for export , but fish bones are also rich in Ca and P in an appropriate proportion, which can be used as an ideal Ca source for humans, and their nutritional value is also very high. A large amount of leftovers such as fish skin and fish bones, wherein fish bones account for about 15% of the leftove...

Claims

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Application Information

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IPC IPC(8): A23L33/18A23L33/16
CPCA23L33/18A23L33/16
Inventor 郭建宋文东纪丽丽王亚宁蔡璐应知伟林艺鸿
Owner ZHEJIANG OCEAN UNIV