Method for directly preparing fresh bdellovibrio bacteriovorus in utilization land of bdellovibrio bacteriovorus and bdellovibrio bacteriovorus culture medium

A technology of Bdellovibrio and Bdellovibrio, which is applied in the field of fermentation, can solve the problems of high price of Bdellovibrio products, limit the application of Bdellovibrio, and high production cost, and achieve simple cultivation methods, easy implementation, and simplified cultivation procedures Effect

Inactive Publication Date: 2018-03-06
山东集大生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the commercially available Bdellovibrio products in my country are composed of water and powder. The fermentation process of the water is simple, but its content is low (generally at 10 5 ~10 9 PFU/mL), the storage time is short (the content decays up to 95% after one month); and the freeze-dried powder preparation has a long storage time and is convenient for transportation, but has the disadvantage of high preparation cost. The price of pure Bdellovibrio freeze-dried powder is

Method used

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Examples

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Effect test

preparation example Construction

[0020] Further, the preparation method of the Bdellovibrio medium comprises: after centrifuging the cultured host bacteria, resuspending the sludge with physiological saline so that the concentration of the host bacteria is 10 9 ~10 14 PFU / mL, packaged after inactivation, can be directly packaged into water, or can be made into powder after spray drying, drying, freeze-drying and other drying processes. The freeze-dried powders are mixed and packaged together. More specifically, resuspend the sludge with physiological saline so that the concentration of the host bacteria is 10 9 ~10 14 After the PFU / mL, it is divided into 5mL, 8mL, 10mL and other vials. After the host bacteria in the vials are inactivated under high temperature and pressure, the vials containing the inactivated host bacteria are sealed and sealed.

[0021] Step S2: then the Bdellovibrio nutrient solution is mixed with the Bdellovibrio bacterial classification to obtain the Bdellovibrio fermentation broth, a...

Embodiment 1

[0035] The present embodiment provides a kind of Bdellovibrio medium N1, which comprises:

[0036] a. Cultivate Escherichia coli as host bacteria: inoculate Escherichia coli in common nutrient broth, and culture on a shaking table.

[0037] b. After the cultivation, centrifuge the host bacteria and resuspend the sludge with physiological saline to make the concentration of the host bacteria 10 9 CFU / mL, and divide it into vials of different specifications.

[0038] c. Inactivate the above-mentioned vial containing the host bacteria at 140° C. for 15 minutes, and seal it with a cap to obtain Bdellovibrio culture medium N1, which is an aqueous preparation.

Embodiment 2

[0040] The present embodiment provides a kind of Bdellovibrio medium N2, which comprises:

[0041] a. Cultivating Rhodopseudomonas palustris as a host bacterium: inoculating Rhodopseudomonas palustris into the culture medium and culturing on a shaking table.

[0042] b. After the cultivation, centrifuge the host bacteria and resuspend the sludge with physiological saline to make the concentration of the host bacteria 10 12 CFU / mL, and divide it into vials of different specifications.

[0043] c. Inactivate the above-mentioned vial containing the host bacteria at 140°C for 15 minutes, freeze-dry it into a powder by freeze-drying method, and seal it with a cap to obtain Bdellovibrio culture medium N2, namely dry powder.

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PUM

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Abstract

The invention provides a method for directly preparing fresh bdellovibrio bacteriovorus in a utilization land of the bdellovibrio bacteriovorus and a bdellovibrio bacteriovorus culture medium and belongs to the field of fermentation. The method for preparing the fresh bdellovibrio bacteriovorus by a farmer comprises the following steps: 0.5 to 15 days before the farmer needs to utilize the bdellovibrio bacteriovorus, mixing the bdellovibrio bacteriovorus culture medium containing host bacteria and water to obtain a bdellovibrio bacteriovorus culture solution; then mixing the bdellovibrio bacteriovorus culture solution with a bdellovibrio bacteriovorus strain to obtain a bdellovibrio bacteriovorus fermented solution; and standing and culturing the bdellovibrio bacteriovorus fermented solution for 12h to 72h at 18 DEG C to 36 DEG C in the presence of air. BY adopting the preparation method, a culture environment of the bdellovibrio bacteriovorus is enlarged, so that the culture of the bdellovibrio bacteriovorus is simple and easy to implement; the bdellovibrio bacteriovorus can be culture while being needed, so that a high death rate of an existing bdellovibrio bacteriovorus water product in a storage or transportation process is avoided; and meanwhile, the utilization cost of bdellovibrio bacteriovorus pure lyophilized powder also can be reduced.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a method for directly preparing fresh Bdellovibrio at the site where the Bdellovibrio is used and a Bdellovibrio culture medium. The culture medium can be liquid or powdered. Background technique [0002] Bdellovibrio is a group of bacteria that parasitize other bacteria and can cause their lysis. It is smaller than normal bacteria, can pass through bacterial filters, and acts like a bacteriophage. The strain size is 0.2~0.5μm×0.8~1.2μm. It is a natural biological control factor for the growth of pathogenic bacteria in the aquaculture water environment. It can effectively control the chemical oxygen consumption and the content of sulfide and ammonia nitrogen in the aquaculture water. New ways of biological control of aquatic animal diseases are of great significance. [0003] At present, the commercially available Bdellovibrio products in my country are composed of water and powder....

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/01
CPCC12N1/04C12N1/20
Inventor 范红照王晓红
Owner 山东集大生物科技有限公司
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