Production method of agricultural gamma-polyglutamic acid

A polyglutamic acid and production method technology, applied in the field of agricultural microorganisms, can solve the problems of high cost of polyglutamic acid, low substrate conversion rate, and ineffective effects, etc., to achieve improved substrate conversion rate, The effect of shortening the fermentation cycle and reducing costs

Inactive Publication Date: 2018-03-06
天津北洋百川生物技术有限公司
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Problems solved by technology

[0004] In the current literature, the increase in the production of polyglutamic acid is mainly through strain mutagenesis and optimization of medium components. Often the effect is not obvious, the fermentation cycle is long, and the conversion rate of the substrate is not high, resulting in high cost of polyglutamic acid. no less

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A production method of agricultural grade gamma-polyglutamic acid, comprising the following steps:

[0043] (1) Strain activation: the original strain of Bacillus licheniformis CGMCC NO.3336 was inoculated on the slant medium, and cultivated at 37°C for 18 hours to prepare mature slant seeds; the composition of the slant medium was: tryptone 10g / L, yeast Dip powder 5g / L, NaCl 10g / L, agar powder 20g / L, and the balance is water.

[0044] Bacillus licheniformis (Bacillus licheniformis) TKPG091, this bacterial strain has been preserved on October 14, 2009 at the General Microbiology Center of the Chinese Academy of Sciences Institute of Microbiology, China Microbiological Culture Collection Management Committee, No. 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No.3336;

[0045] (2) Preparation of seed solution: Inoculate the activated slant seeds into a 500mL Erlenmeyer flask containing 50mL of liquid medium, cultivate at 37°C at a shaker s...

Embodiment 2

[0051] A production method of agricultural grade gamma-polyglutamic acid, comprising the following steps:

[0052] (1) Strain activation: the original strain of Bacillus licheniformis was inoculated on the slant medium, and cultured at 30°C for 16 hours to prepare activated slant seeds; the composition of the slant medium was: tryptone 10g / L, yeast extract powder 5g / L, NaCl10g / L, agar powder 20g / L, and the balance is water.

[0053] Bacillus licheniformis (Bacillus licheniformis) TKPG091, this bacterial strain has been preserved on October 14, 2009 at the General Microbiology Center of the Chinese Academy of Sciences Institute of Microbiology, China Microbiological Culture Collection Management Committee, No. 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No.3336;

[0054] (2) Preparation of seed liquid; Inoculate the activated slant seeds in a 500mL Erlenmeyer flask with 50mL of liquid medium, cultivate at 37°C at a shaker speed of 220rpm for 1...

Embodiment 3

[0060] A production method of agricultural grade gamma-polyglutamic acid, comprising the following steps:

[0061] (1) Strain activation: the original strain of Bacillus licheniformis was inoculated on the slant medium, and cultured at 35°C for 17 hours to prepare activated slant seeds; the composition of the slant medium was: tryptone 10g / L, yeast extract powder 5g / L, NaCl10g / L, agar powder 20g / L, and the balance is water.

[0062]Bacillus licheniformis (Bacillus licheniformis) TKPG091, this bacterial strain has been preserved on October 14, 2009 at the General Microbiology Center of the Chinese Academy of Sciences Institute of Microbiology, China Microbiological Culture Collection Management Committee, No. 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No.3336;

[0063] (2) Preparation of seed solution; inoculate the activated slant seeds in step (1) in a 500mL Erlenmeyer flask with 50mL of liquid medium, cultivate at 37°C at a shaker speed of...

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Abstract

The invention relates to a production method of agricultural gamma-polyglutamic acid, comprising the following steps: strain activation; inoculating original strain of Bacillus licheniformis to a slant culture-medium for culture activation; preparation of a seed solution; inoculating activated seeds to a seed medium to obtain a gamma-polyglutamic acid seed solution; inoculating the seed solution to a fermentation medium for fermentation; and centrifuging to remove thalline after the fermentation, carrying out alcohol precipitation to obtain polyglutamic acid, and drying so as to prepare yellowpowdery agricultural polyglutamic acid. Through a laboratory study on fermentation of agricultural polyglutamic acid, it is found that potassium salts have a great effect of promoting synthesis of agricultural polyglutamic acid. By adding potassium chloride and raising agricultural output, conversion rate of a substrate is raised. The method is convenient to operate. By the method, output is increased, and cost of later polyglutamic acid extraction is reduced in comparison with a production method of cosmetic polyglutamic acid.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and relates to a production method of agricultural grade γ-polyglutamic acid. Background technique [0002] γ-polyglutamic acid is a multifunctional biodegradable polymer material formed by combining D-glutamic acid and L-glutamic acid through the amide bond between the α-amino group and the γ-carboxyl group. The molecular weight distribution is between 100kDa and 10000kDa. γ-polyglutamic acid has excellent water solubility, super adsorption and biodegradability, and the degradation product is pollution-free glutamic acid. It is an excellent environment-friendly polymer material and can be used as a water-retaining agent, heavy metal Ionic adsorbents, flocculants, slow-release agents and drug carriers, etc., have high commercial and social values ​​in cosmetics, environmental protection, food, medicine, agriculture, desert control and other industries. [0003] It has only b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C12R1/10
CPCC12P13/02
Inventor 乔长晟张卫汪建英李雪李振海
Owner 天津北洋百川生物技术有限公司
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