Method for detecting components of herba erigerontis through high performance liquid chromatography

A high-efficiency liquid phase and scutellaria technology, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of few researches, complex methods, and inability to quantify, and achieve good repeatability, resource saving, and easy operation Effect

Active Publication Date: 2018-03-09
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Erigeron breviscapus has good medicinal value and a broad space for the development of the pharmaceutical market, but there are not many studies on the detection of multiple components at the same time. Hong Bo et al. extracted and separated 7 compounds from Erigeron breviscapus (Study on the chemical composition of Erigeron breviscapus), and Li Jing et al. isolated 12 compounds from Erigeron breviscapus (Research on the chemical constituents of breviscapi chinensis), some of which were identified for the first time, and there are methods Complicated, can not be fully quantified from Breviscapus and other shortcomings

Method used

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  • Method for detecting components of herba erigerontis through high performance liquid chromatography
  • Method for detecting components of herba erigerontis through high performance liquid chromatography
  • Method for detecting components of herba erigerontis through high performance liquid chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The equipment used is, including pump, degassing device, sample injector, detector, column thermostat, workstation, chromatographic column, balance, reference substance, the experimental water is ultrapure water, phosphoric acid is analytical pure, methanol and acetonitrile are chromatographic pure .

[0037] Select octadecylsilane bonded gel as filler, take acetonitrile as mobile phase A phase, methanol and acetonitrile as B phase, gradient elution, detect described test sample solution and contrast sample solution in liquid chromatograph, Component content obtained by external standard method analysis;

[0038] Select HPLC-VWD;

[0039] Select chromatographic column product parameters: chromatographic column without guard column EC-C18 2.7μm;

[0040] Set the flow rate to 1.000mL / min;

[0041] Set 0.2% phosphoric acid + 5% acetonitrile as mobile phase A, 0.2% phosphoric acid + 40% methanol + 40% acetonitrile as mobile phase B, and the gradient is shown in the table...

Embodiment 2

[0049] Used chromatograph, filling agent, mobile phase are identical with embodiment 1.

[0050] Take Yangbi from the place of origin for the test sample solution and inject it into the liquid phase bottle, inject the sample, record the chromatogram, and calculate the content using the external standard method. By calculating the peak area ratio of the need testing solution and the reference substance solution, the content of 10 components of the need testing solution can be obtained, and the result table of each test sample is as follows in table 4. figure 2 .

[0051]

[0052]

[0053] According to the above method, the detection method of Example 2 is verified, and the results show that the specificity of the present invention, repeatability, precision, and applicability all meet the requirements

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Abstract

The invention belongs to the field of medicines and in particular relates to a method for detecting components of herba erigerontis through a high performance liquid chromatography. The detection method provided by the invention comprises the following steps: step (a) providing 10 types of sample reference substance solutions and test sample solutions; (b) analyzing the content of 10 types of components in the test sample solution, wherein the high performance liquid chromatography is adopted, an octadecyl silane bonding adhesive is used as a filling agent and chromatographic conditions are asfollows: a chromatographic column has no protection column EC-C18 with the size of 2.7mu m, takes 0.2 percent of phosphoric acid and 5 percent of acetonitrile as a mobile phase A and 0.2 percent of phosphoric acid, 40 percent of methanol and 40 percent of acetonitrile as an organic phase B; carrying out 6 times of linear gradient transformation and elution, wherein the flow speed is 1.000mL / min,the column temperature is 40 DEG C and the detection wavelength is 335nm; determining the 10 types of components; stopping for 30min and then operating for 6min. According to the method provided by the invention, the components of the herba erigerontis are simultaneously detected by the existing high performance liquid chromatography; the method has the advantages of strong specificity, rapidnessand flexibility, accuracy and good precision. When the method is used for detecting, the detection efficiency of the components of the herba erigerontis can be improved and resources are saved.

Description

technical field [0001] The invention proposes a method for detecting components of scutellaria breviscapus by high performance liquid phase. Background technique [0002] Erigeron breviscapus (Vant.) Hand-Mazz is the dry whole herb of Compositae. It is also known as Erigeron breviscapus (Vant.) Hand-Mazz. It is mainly distributed in southwest China, especially in Yunnan. It has the effects of promoting blood circulation, dredging collaterals and relieving pain, expelling wind and cold. Scutellarin can achieve its obvious protective effect on acute cerebral ischemia through multiple mechanisms: it can inhibit platelet aggregation, inhibit coagulation function in vivo and promote fibrinolytic activity, thereby inhibiting thrombus formation; Vasospasm shrinks, increases local cerebral blood flow, improves cerebral microcirculation, reduces inflammatory cell adhesion and infiltration, and protects brain tissue after ischemia. These effects are beneficial to the treatment and pr...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/047G01N2030/884
Inventor 张广辉赵明杨生超龙光强念波李锐陆迎春马燕宋婉玲蒋宾
Owner YUNNAN AGRICULTURAL UNIVERSITY
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