Preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor

A multi-drug resistance, nanoparticle technology, applied in the fields of pharmacy, materials science and oncology, can solve the problems of increased drug concentration and low drug loading rate of nanoparticles, and achieve increased concentration, overcome tumor MDR, and encapsulation efficiency. high effect

Active Publication Date: 2018-03-23
ZHEJIANG UNIV
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Problems solved by technology

However, there are problems in this kind of drug loading by adsorption: first, the drug loading rate of nanoparticles is not high, and the drug is easily released from the extracellular nanoparticles so that the drug exists in a free form, so that the carrier fails to play the role of overcoming tumor MDR; The second is that the carrier overcom

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  • Preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor
  • Preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor
  • Preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor

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[0033] Example 1

[0034] Blank TiO 2 Preparation of nanoparticles: Weigh 0.4g NaOH, add 50mL deionized water and stir to dissolve it, add TiO 2 Nano-particle powder 200mg, 400W ultrasonic for 15min, transferred to a hydrothermal kettle with a polytetrafluoroethylene liner, screwed on the lid of the kettle, heated in the drying box at the initial temperature of 60℃, and continued to heat for 36h after the temperature reached 150℃. After cooling to room temperature, wash with 0.1M HCl solution, centrifuge, add ammonium sulfate until the pH is 5, dry the sample at 60°C, and store it in the dark after grinding.

[0035] Preparation of doxorubicin TiO 2 Nanoparticles: the obtained blank TiO 2 Nanoparticles passed through Sephadex G-50 column, eluted with pH7.2 phosphate buffer solution and collected TiO 2 For the nano part, add 1 mL of 200mg / mL doxorubicin solution, load at 60°C for 30 minutes, eluted with pH 7.2 phosphate buffer solution and pass through Sephadex G-50 gel column to rem...

Example Embodiment

[0043] Example 2

[0044] Change doxorubicin to epirubicin, and the others are the same as in Example 1, to obtain epirubicin TiO 2 Nanoparticles have an encapsulation rate of (76.3±3.5)%, a particle size of (358±79) nm, and a Zeta potential of (-20±5) mV.

Example Embodiment

[0045] Example 3

[0046] Change ammonium sulfate to other pH regulators such as phosphate buffer, diammonium hydrogen phosphate and sodium hydroxide, and the others are the same as in Example 1, to obtain doxorubicin TiO 2 Nanoparticles, the encapsulation efficiency is (67.9±4.6)%~(75.7±3.8)%, and the particle size is (265±78)nm~(396±89)nm.

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Abstract

The invention discloses a preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor. The preparation method comprises the steps of forming empty TiO2 nanoparticles with effective pH gradient, encapsulating anthracycline antitumor drugs into the TiO2 nanoparticles, and thus preparing the anthracycline anti-tumor drug TiO2 nanoparticles. The preparation method hasthe advantage of simple preparation, can significantly improve the encapsulation efficiency of the anthracycline antitumor drug, and effectively omits recognition, binding and discharging of multidrugresistance related proteins of drug-resistant cells through the encapsulation technology, so as to effectively enhance the drug amount of drugs in the drug-resistant cells, prolong the drug retentionin the drug-resistant cells and indicate relatively good tumor MDR-overcoming effect. Compared with other methods, the method provided by the invention is simple and convenient, can delay the releaseof the drugs in a culture medium, and can co-carry other tumor MDR-overcoming drugs, has low requirements for preparation materials, has high encapsulation efficiency, and can effectively overcome multi-drug resistance.

Description

technical field [0001] The invention belongs to the fields of pharmacy, material science and oncology, and specifically relates to a preparation method of a drug carrier capable of overcoming multi-drug resistance of tumors. Background technique [0002] Multidrug resistance (MDR) is common in anticancer drugs and has been the main reason for the failure of chemotherapy. How to effectively overcome MDR has always been one of the important issues for researchers in various fields of biomedicine. MDR is an important cellular defense mechanism for tumor cells to avoid drug attacks. It refers to the phenomenon that tumor cells are resistant to one chemotherapeutic drug and also have cross-resistance to other drugs that have nothing to do with its structure and have different mechanisms of action. MDR can reduce Drug absorption, increased drug efflux, increased rate of drug metabolism, or changes in drug target properties. [0003] In recent years, drug carriers such as liposome...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/02A61P35/00A61K31/704A61K31/277
CPCA61K9/5115A61K31/277A61K31/704A61K2300/00
Inventor 许东航高建青安全福胡宗权
Owner ZHEJIANG UNIV
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