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Preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor

A multi-drug resistance, nanoparticle technology, applied in the fields of pharmacy, materials science and oncology, can solve the problems of increased drug concentration and low drug loading rate of nanoparticles, and achieve increased concentration, overcome tumor MDR, and encapsulation efficiency. high effect

Active Publication Date: 2018-03-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems in this kind of drug loading by adsorption: first, the drug loading rate of nanoparticles is not high, and the drug is easily released from the extracellular nanoparticles so that the drug exists in a free form, so that the carrier fails to play the role of overcoming tumor MDR; The second is that the carrier overcomes the research mechanism of tumor MDR by effectively "escaping" the recognition of MDR-related proteins, and the drug adsorbed on the surface of the nanoparticle or the drug that has been released may not be able to escape the recognition of MDR-related proteins, resulting in a limited increase in the concentration of the drug entering the cell

Method used

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  • Preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor
  • Preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor
  • Preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor

Examples

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Effect test

Embodiment 1

[0034] Blank TiO 2 Preparation of nanoparticles: Weigh 0.4g of NaOH, add 50mL of deionized water and stir to dissolve, add TiO 2 200 mg of nanoparticle powder, 400W ultrasonic for 15 minutes, transferred to a hydrothermal kettle with a polytetrafluoroethylene liner, tightened the lid of the kettle, started to heat up in the drying oven at an initial temperature of 60°C, and continued heating for 36h after the temperature was raised to 150°C. After cooling to room temperature, wash with an appropriate amount of 0.1M HCl solution, centrifuge, add ammonium sulfate until the pH is 5, dry the sample at 60°C, grind and store in the dark.

[0035] Preparation of Doxorubicin TiO 2 Nanoparticles: the resulting blank TiO 2 Nanoparticles passed through Sephadex G-50 column, eluted with pH7.2 phosphate buffer solution and collected TiO 2 Add 1mL of 200mg / mL doxorubicin solution to the nanometer part, load at 60°C for 30min, elute with pH 7.2 phosphate buffer solution and pass through S...

Embodiment 2

[0044] Doxorubicin is changed into epirubicin, and other is with embodiment 1, obtains epirubicin TiO 2 Nanoparticles, the encapsulation rate is (76.3±3.5)%, the particle size is (358±79)nm, and the Zeta potential is (-20±5)mV.

Embodiment 3

[0046] Ammonium sulfate is changed into other pH regulators such as phosphate buffer, diammonium hydrogen phosphate and sodium hydroxide, and other is with embodiment 1, obtains doxorubicin TiO 2 The encapsulation efficiency of nanoparticles is (67.9±4.6)%~(75.7±3.8)%, and the particle size is (265±78)nm~(396±89) nm.

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Abstract

The invention discloses a preparation method of TiO2 nanoparticles capable of overcoming multidrug resistance of tumor. The preparation method comprises the steps of forming empty TiO2 nanoparticles with effective pH gradient, encapsulating anthracycline antitumor drugs into the TiO2 nanoparticles, and thus preparing the anthracycline anti-tumor drug TiO2 nanoparticles. The preparation method hasthe advantage of simple preparation, can significantly improve the encapsulation efficiency of the anthracycline antitumor drug, and effectively omits recognition, binding and discharging of multidrugresistance related proteins of drug-resistant cells through the encapsulation technology, so as to effectively enhance the drug amount of drugs in the drug-resistant cells, prolong the drug retentionin the drug-resistant cells and indicate relatively good tumor MDR-overcoming effect. Compared with other methods, the method provided by the invention is simple and convenient, can delay the releaseof the drugs in a culture medium, and can co-carry other tumor MDR-overcoming drugs, has low requirements for preparation materials, has high encapsulation efficiency, and can effectively overcome multi-drug resistance.

Description

technical field [0001] The invention belongs to the fields of pharmacy, material science and oncology, and specifically relates to a preparation method of a drug carrier capable of overcoming multi-drug resistance of tumors. Background technique [0002] Multidrug resistance (MDR) is common in anticancer drugs and has been the main reason for the failure of chemotherapy. How to effectively overcome MDR has always been one of the important issues for researchers in various fields of biomedicine. MDR is an important cellular defense mechanism for tumor cells to avoid drug attacks. It refers to the phenomenon that tumor cells are resistant to one chemotherapeutic drug and also have cross-resistance to other drugs that have nothing to do with its structure and have different mechanisms of action. MDR can reduce Drug absorption, increased drug efflux, increased rate of drug metabolism, or changes in drug target properties. [0003] In recent years, drug carriers such as liposome...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/02A61P35/00A61K31/704A61K31/277
CPCA61K9/5115A61K31/277A61K31/704A61K2300/00
Inventor 许东航高建青安全福胡宗权
Owner ZHEJIANG UNIV
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