Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A preparation method of sea urchin-shaped gold nanoparticles and a method for labeling proteins

A sea urchin-shaped and nano-gold technology is applied in the field of nano-gold particle labeling protein and the preparation of sea urchin-shaped nano-gold particles, which can solve the problems of being difficult to obtain, prone to agglomeration and flocculation, and contradictory, and achieve the effect of easy reconstitution.

Active Publication Date: 2020-06-09
SHIHEZI UNIVERSITY
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some methods need to synthesize seeds in advance, which makes the synthesis steps more cumbersome and time-consuming, while others require the use of toxic surfactants or macromolecular polymers, which are not only difficult to obtain but also have poor biocompatibility, which is contrary to the concept of green chemistry.
[0004] Sea urchin-shaped gold nanoparticles are widely used as an ideal SERS substrate due to their special surface energy to generate abundant hot spots. However, the existing sea urchin-shaped gold nanoparticles are not stable after synthesis and are prone to agglomeration and flocculation; The application cannot directly couple biomolecules such as protein and nucleic acid like colloidal gold
[0005] In addition, sea urchin-shaped gold nanoparticles are very different from ordinary colloidal gold, especially the surface charge properties. There are certain obstacles in labeling biomolecules such as proteins. Sea urchin-shaped gold nanoparticles are easy to agglomerate in biological buffers. There is no way to reconstitute dead gold in the buffer to form aggregates, limiting further applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method of sea urchin-shaped gold nanoparticles and a method for labeling proteins
  • A preparation method of sea urchin-shaped gold nanoparticles and a method for labeling proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: A kind of preparation method of sea urchin shape nano-gold particle is characterized in that mainly comprises step: in N-(2-hydroxyethyl) piperazine-N'-2-ethanesulfonic acid solution, add chloroauric acid The solution was fully stirred and mixed, and slowly stirred until it changed from colorless and transparent to dark blue, then stopped stirring for 10 minutes to fully complete the reaction, then added citric acid or potassium carbonate to adjust the pH value, and adjusted the pH value to 6.5.

[0030]Add 2 parts by weight of the above-mentioned N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid solution, the concentration is 0.1mol / L, and the pH is 7.4; the added chloroauric acid solution is 1.0 parts by weight , the concentration is 0.03mol / L.

[0031] In actual use: Add citric acid or potassium carbonate to adjust the pH value to determine the pH value based on the principle of isoelectric point value of the labeled protein + 1, for example: the is...

Embodiment 2

[0032] Embodiment 2: A kind of preparation method of sea urchin-shaped gold nanoparticles is characterized in that it mainly includes the steps of adding chloroauric acid in N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid solution The solution was fully stirred and mixed, and slowly stirred until it changed from colorless and transparent to dark blue, then stopped stirring for 25 minutes to fully complete the reaction, then added citric acid or potassium carbonate to adjust the pH value, and adjusted the pH value to 8.5.

[0033] Add 1 part by weight of the above N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid solution, the concentration is 0.06mol / L, and the pH is 7.2; the added chloroauric acid solution is 0.5 parts by weight , the concentration is 0.02mol / L.

[0034] In actual use: Add citric acid or potassium carbonate to adjust the pH value to determine the pH value based on the principle of isoelectric point value of the labeled protein + 1, for example: the ...

Embodiment 3

[0035] Embodiment 3: A kind of preparation method of sea urchin-shaped gold nano particles is characterized in that it mainly includes the steps of: adding chloroauric acid in N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid solution The solution was fully stirred and mixed, and slowly stirred until it changed from colorless and transparent to dark blue, then stopped stirring for 15 minutes to fully complete the reaction, then added citric acid or potassium carbonate to adjust the pH value, and adjusted the pH value to 5.5.

[0036] Add 5 parts by weight of the above-mentioned N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid solution, the concentration is 0.08mol / L, and the pH is 7.5; the added chloroauric acid solution is 0.7 parts by weight , the concentration is 0.01mol / L.

[0037] In actual use: Add citric acid or potassium carbonate to adjust the pH value to determine the pH value based on the principle of isoelectric point value of the labeled protein +1, for e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of urchin-like nano gold particles. The preparation method is characterized by mainly comprising the following steps: adding a chloroauric acid solution into an N-(2-ethoxy) piperazine-N'-2-ethanesulfonic acid solution, carrying out full and uniform stirring and mixing, slowly stirring the solution till the solution turns from colorless transparency into a dark blue color, stopping stirring, stewing for 10 to 25 minutes to fully complete the reaction, and then adding citric acid or potassium carbonate to adjust the pH value to be 5.5 to 8.5. Compared with the prior art, the preparation method has the advantages of quickness, no need of gold seeds, green synthesis and the like.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a method for preparing sea urchin-shaped gold nano particles and a method for marking proteins with the gold nano particles. Background technique [0002] Due to their unique optical, electrical, magnetic, and catalytic properties, nanoparticles have broad application prospects in the fields of catalysis, electronics, optics, and biomedicine, and thus have received extensive attention. Nanoparticles with uneven and spiny structures are an important part of three-dimensional nanomaterials, and they have various names according to their classification, such as star, multipod, and concave-convex gold particles. Because of its rough surface and high specific surface area, it can enhance the plasmon resonance in the near-infrared region and concentrate the electromagnetic field enhancement effect on its tip, so that there are many hot spots on the same nanoparticle surf...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/13
CPCB22F9/24B82Y40/00C07K1/13
Inventor 石峰陈创夫王远志祝明松张洁李相如
Owner SHIHEZI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com