Bilayered devices for enhanced healing
A gel layer, cross-linked layer technology, applied in the field of double-layer devices, can solve problems such as disrupting epithelial cell migration, increasing costs and patient discomfort
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0100] Smooth (SM) and micropatterned (+1.7SK2x2) samples were prepared by casting polydimethylsiloxane elastomer (Xiameter RTV-4232-T2, Dow Corning; PDMSe) through low negative silicon wafer molds. Circular samples (d = 20 mm) were adhered to 12-well plates with features aligned parallel to the direction of cell migration and treated with fibronectin (15 μg / mL overnight) to promote cell attachment. A SM PDMSe rectangle (5 mm x 20 mm) was placed along the center of the sample to generate a modified scratch assay. Human epidermal keratinocytes (HEK) were seeded on the entire configuration at 1 × 104 cells / cm2 and maintained in complete keratinocyte growth medium (dermal cell basal medium, 0.4% bovine pituitary extract, 0.5 ng / ml rhTGF-α, 6 mM L-glutamine, 100 ng / ml hydrocortisone, 5 μg / ml insulin, 1 μM epinephrine, 5 μg / ml apolipoprotein transporter, 50 U / ml penicillin / streptomycin and 1 μg / ml Fungizone antifungal agent). At approximately 70% confluence, the PDMSe rectangle w...
example 2
[0107] As the understanding of the intricacies of wound healing evolves, the wound dressings market grows accordingly. The requirements to replace lost skin depend largely on the type and extent of the wound, as the damage results in varying degrees of loss of skin structure. Superficial wounds result in loss of the outermost layer of the skin or epidermis. This layer maintains the barrier function of the skin and is mainly composed of epithelial cells called keratinocytes. In more severe injuries, loss of the epidermal layer and part or all of the dermis (eg, a partial or full wound, respectively) results in the need for replacement or reconstruction of two important tissue types to restore skin integrity. The dermis is the connective tissue responsible for the mechanical properties of the skin. It is mainly composed of fibroblasts and their associated extracellular matrix (ECM). Skin wound healing proceeds in four precisely integrated phases: hemostasis; inflammation; pro...
example 3
[0112] The top performing Sharklet micropattern (+10SK50x50) from the HEK migration assay was scaled up to an appropriate size for use in wound dressings and tested in a delayed healing model established in rats to validate a broad range of non-healing / chronic Wound healing behavior. The top layer of the wound dressing prototype was produced following the same procedure as above to prepare the gelatin hydrogel. The solution-filled molds were then cooled to 4°C for 1 hour to solidify the gelatin. Next, synthesize 2 ml of CAPGEL as previously described TM slurry and loaded it into a PDMSe mold (d=20mm) on top of solid gelatin. Place the top gelatin layer and the base CAPGEL TM Layers were chemically crosslinked with a solution of 0.6M EDC and 0.2M NHS in PBS at 4°C for 18 hours. The silicone mold was removed from the construct and an appropriately sized dressing was prepared using a 6-mm biopsy punch. Dressings were then washed in PBS for 1 hour, followed by three changes o...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More