Method for determining monosaccharide constitution of Yupingfeng polysaccharide
A composite polysaccharide, jade screen technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that it cannot be directly measured, polysaccharides and monosaccharides do not have ultraviolet absorption, etc., and achieves good separation, high sensitivity, and stability. good effect
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[0019] A method for measuring the monosaccharide composition of Yupingfeng complex polysaccharide, comprising the steps of:
[0020] 1) Preparation of Yupingfeng composite crude polysaccharide: Take medicinal Astragalus root, Atractylodes macrocephala, and Fangfeng in a weight ratio of 3:1:1 and mix them as materials; ℃, extraction times 3 times, after suction filtration, concentration of the extract, ethanol precipitation and concentration for 12 hours, the precipitate was vacuum-dried to obtain the Yupingfeng compound crude polysaccharide;
[0021] 2) Preparation of Jade Pingfeng compound refined polysaccharide: take the Jade Pingfeng compound refinement obtained in step 1), purify and concentrate with AB-8 macroporous resin, and then vacuum freeze-dry to obtain Jade Pingfeng compound refinement polysaccharide;
[0022] 3) Prepare the hydrolyzed sample of Yupingfeng compound polysaccharide: take 50 mg of Yupingfeng compound refined polysaccharide and mix it with 2 mL of 2 mo...
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