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A kind of flavonoid carbon glycoside composition of cloth dregs leaves and its preparation method and application

A kind of flavonoid carbon glycoside composition of cloth dregs leaf and the technology of flavonoid carbon glycoside composition of dregs leaf, applied in the field of flavonoid carbon glycoside composition of cloth dregs leaf and its preparation, can solve the problems such as lack of effective treatment of ALI, and achieve improvement of acute lung injury , The components are safe and reliable, and the effect of good application prospects

Active Publication Date: 2021-03-23
GUANGDONG PHARMA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, there is still a lack of effective drugs for the treatment of ALI

Method used

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  • A kind of flavonoid carbon glycoside composition of cloth dregs leaves and its preparation method and application
  • A kind of flavonoid carbon glycoside composition of cloth dregs leaves and its preparation method and application
  • A kind of flavonoid carbon glycoside composition of cloth dregs leaves and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0035] according to figure 1 The process shown is for extracting the flavonoid carbon glycoside composition of Buza leaves. Take 5 kg of cloth residue leaves, add 80 L of deionized water, heat and reflux for extraction twice, each time for 2 hours. The extract was concentrated, centrifuged at 3000 r / min for 10 min, and the supernatant was adjusted to about 10 L, then adsorbed on a D101 or AB-8 macroporous resin column, and then sequentially washed with deionized water, 10% ethanol and 70% ethanol For elution, concentrate the 70% ethanol eluate and hydrolyze it with 5% HCl-methanol at 80°C for 2 hours, neutralize, concentrate, and apply Sephadex LH-20 column chromatography; wash with 80% methanol The eluate was analyzed by HPLC, and the fractions containing flavonoid glycosides were combined and concentrated to obtain total flavonoid glycosides; then separated by preparative chromatography (C18 column), gradient eluted with water and methanol, and the eluate was detected by a...

Embodiment 2

[0037] Example 2: Buzaye Flavonoid Carbon Glycoside Composition (ACGs) Improves LPS-Induced Acute Lung Injury Experiment in Mice

[0038] Experimental drugs and reagents: lipopolysaccharide ( E. coli 055:B5 , Sigma Company), MPO kit (Nanjing Jiancheng), Giemsa staining kit (Nanjing Jiancheng), BCA protein quantification kit (Biyuntian).

[0039] Experimental animals: SPF grade Balb / c mice, male, 8-10 weeks old, provided by the Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine, animal license number: SCXK (Guangdong) 2013-0034.

[0040] experimental method:

[0041] Ninety male Balb / C mice aged 8-10 weeks were reared at a temperature of 22°C-24°C, a humidity of 60%-65%, 12 hours of light, and free access to food and water. After feeding for 7 days, they were randomly divided into 6 groups, 12 in each group, and divided into 6 groups, namely the control group, LPS group, LPS+ Buzaye flavonoid carbon glycoside composition (ACGs) with high, medi...

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Abstract

The invention provides a microcos paniculata flavone carbon glycoside compound and a preparation method and application thereof. The preparation method of the microcos paniculata flavone carbon glycoside compound comprises the steps that macroporous resin adsorbs a water extract of the microcos paniculata, after elution is conducted sequentially through water and 10% ethyl alcohol, 70% ethyl alcohol elution fraction is collected, the fraction is subjected to acid hydrolysis and neutralization to obtain a transition product; and the transition product is eluted by using 80% methyl alcohol, andthe microcos paniculata flavone carbon glycoside compound is obtained through separation and purification. The microcos paniculatat flavone carbon glycoside compound is prepared from the following components of vitexin, isovitexin, apigenin-6,8-C-diglucoside, apigenin-6-C-arabinose-8-C-glucoside, apigenin-6-C-glucose-8-C-arab glycoside, apigenin-6-C-xylose-8-C-glucoside, apigenin-6-C-glucose-8-C-rhamnoside, and apigenin-6-C-rhamnose-8-C-glucoside, wherein the content of apigenin carbon glycoside compounds accounts for 50% or more of the mass of the flavone carbon glycoside compound. The microcos paniculata flavone carbon glycoside compound can effectively improve acute lung injury of a model mouse caused by LPS, and has relative good application prospects on development of drugs for treating acute lung injury and lung inflammation.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines and natural medicines, and relates to flavonoid carboglycosides derived from traditional Chinese medicines, and more specifically to a composition of flavonoid carboglycosides from cloth dregs leaves and its preparation method and application. Background technique [0002] Acute lung injury (ALI) is the damage of alveolar epithelial cells and capillary endothelial cells caused by various direct and indirect injury factors, resulting in diffuse pulmonary interstitial and alveolar edema, leading to acute hypoxic respiratory insufficiency. ALI developed to a severe stage is called acute respiratory distress syndrome (acute respiratory distress syndrome, ARDS). It is currently believed that a variety of effector cells are involved in the process of lung injury. Among them, the activation of macrophages and the aggregation of neutrophils promote the release of pro-inflammatory factors, ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/24A61K31/352A61K31/7048A61P11/00A61K127/00
CPCA61K31/352A61K31/7048A61K36/24A61K2236/331A61K2236/55A61K2300/00
Inventor 李坤平刘芸陈艳芬
Owner GUANGDONG PHARMA UNIV
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