Method for increasing apis cerana smith pollination efficiency for strawberries in winter facility
A technology of Apis chinensis and strawberry, applied in the field of bioengineering, can solve the problems of strawberry production and economic value impact, low efficiency of bee pollination in winter facilities, etc.
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Embodiment 1
[0019] Example 1: Effect of middle bee feeding treatment on improving honeybee activity
[0020] 1.1. Materials and methods. Since wild Chinese bees are more active and there is still surplus honey due to the native method of drum feeding, this example mainly tests whether feeding will affect the pollination activity of Chinese bees. The Chinese honey bee colony used in the experiment was purchased from Jiande Mountain, Hangzhou, and the queen bee in the middle was the new king of autumn reproduction, which was bred by the regular return soil method and the round wooden barrel method. The strawberry variety used in the experiment was "Fengxiang".
[0021] 1.2 Experimental design
[0022] During the test, the bee bucket was placed in the middle of the whole greenhouse, and the greenhouse was divided into three equal parts horizontally with the bee bucket as the middle point. The two ends were the control and treatment areas, and the middle was the buffer zone. The height of ...
Embodiment 2
[0029] Embodiment 2: The effect of middle bee feeding treatment on improving honeybee activity
[0030] 2.1 Materials and methods
[0031] 2.1.1 Tested insects
[0032] Apis chinensis is a population raised artificially in this laboratory. The feeding conditions are: temperature (26±1)°C, relative humidity (75±5)%, photoperiod L:D=12:12 (light 12h, dark 12h). After pupation and eclosion, the sexually mature females were collected for experimentation, and starved for 3 hours before the experimentation.
[0033] 2.1.2 Experimental instruments and reagents
[0034] (1) Experimental equipment
[0035] The antenna potentiometer is produced by Syntech Company in the Netherlands, and consists of four parts: intelligent data acquisition controller IDAC-2, stimulating airflow controller (Syntech CS-55), micro-manipulator (Syntech MN-151) and Syntech software processing system.
[0036] (2) Reagents. Various experimental standard products were purchased from Bailingwei Company. C...
Embodiment 3
[0051] Embodiment 3: the method for spraying strawberry pollination with compound sugar water olfactory active agent formula.
[0052] As mentioned above, Chinese bees have higher olfactory responses to plant floral volatiles and bee pheromones. Therefore, this study treated them with β-ionone and methyl p-hydroxybenzoate, and components of strawberry floral fragrance, respectively. Bee swarms or flowers to study the effect of olfactory active agents on bee pollination behavior.
[0053] 3.1 Materials and methods
[0054] 3.1.1 Tested insects
[0055] The insects used in the test were the same as before, and the bee colony used was purchased from Jiande Mountain, Hangzhou, and was bred by the common return soil method in round wooden barrels. The strawberries used in the experiment were still provided by the Agricultural Biotechnology Research Institute of Hangzhou Academy of Agricultural Sciences. The experiment was carried out at the greenhouse base of the Institute of Ag...
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