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Method for preparing peanut polypeptide solution by utilizing peanut intrinsic protease to hydrolyze peanut protein

A technology of protease hydrolysis and peanut polypeptide is applied in the processing field of vegetable protein and oil, which can solve the problems of low economic value and achieve the effect of low processing cost, healthy nutrition and good function.

Active Publication Date: 2018-04-20
南京爱美倍健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main product form of peanut protein is hot-pressed and cold-pressed cake (or crushed cake), with low economic value

Method used

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  • Method for preparing peanut polypeptide solution by utilizing peanut intrinsic protease to hydrolyze peanut protein
  • Method for preparing peanut polypeptide solution by utilizing peanut intrinsic protease to hydrolyze peanut protein
  • Method for preparing peanut polypeptide solution by utilizing peanut intrinsic protease to hydrolyze peanut protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Soak the peanuts for 12 hours, remove the red coat, remove mildew and deterioration of peanut kernels, rinse, add water of 6 times the mass of peanut kernels, refining for 2 minutes, filter to obtain peanut pulp and peanut residue; peanut pulp is centrifuged at 9600 rpm for 3 minutes, Obtain low-fat peanut pulp and peanut oil body enrichment; adjust the pH of low-fat peanut pulp to 2 with hydrochloric acid, activate at 50℃ for 1h, adjust pH to 2.5 with sodium bicarbonate, and keep it at 60℃ for 48h, Obtain low-fat peanut polypeptide liquid; add apple juice according to the ratio of apple juice / low-fat peanut polypeptide liquid=1 / 9, mix, homogenize, sterilize (90℃, 10min), and package to obtain apple juice peanut polypeptide liquid beverage. The centrifuge is a three-phase centrifuge or a disc centrifuge.

Embodiment 2

[0024] Soak the peanuts for 15 hours, remove the red coat, remove moldy and deteriorated peanut kernels, rinse, add water of 4 times the quality of peanut kernels, refining for 2 minutes, filter to obtain peanut pulp I and peanut residue I; add water to peanut residue I , Peanut pulp I: water mass ratio is 1:3, refining for 1 min to obtain peanut pulp II and peanut pulp II; peanut pulp I and peanut pulp II are mixed to obtain peanut pulp III; peanut pulp III is centrifuged at 7000 rpm for 3 minutes, Obtain low-fat peanut syrup and peanut oil body enrichment; adjust the pH of low-fat peanut syrup to 1.8 with phosphoric acid and activate it at 40℃ for 2h; adjust the pH to 2.3 with sodium carbonate and keep it at 55℃ for 55h to obtain low-fat Peanut polypeptide liquid: add peach juice at the ratio of peach juice / low-fat peanut polypeptide liquid=1 / 9, mix, homogenize, sterilize (95℃, 5min), and pack to obtain peach juice peanut polypeptide liquid beverage. The centrifuge is a three...

Embodiment 3

[0026] Soak the peanuts for 15h, remove the red coat, remove moldy and deteriorated peanut kernels, rinse, add water of 6 times the mass of peanut kernels, refining for 2 minutes, filter to obtain peanut pulp I and peanut residue I; add water to peanut residue I , Peanut pulp I: water mass ratio 1:3, refining for 1.5 minutes to obtain peanut pulp II and peanut pulp II; peanut pulp I and peanut pulp II are mixed to obtain peanut pulp III; peanut pulp III is centrifuged at 5000 rpm for 5 minutes To obtain low-fat peanut pulp and peanut oil body enrichment; adjust the pH of low-fat peanut pulp to 3 with hydrochloric acid, and activate it at 60℃ for 1.5h; without adjusting pH, keep it at 55℃ for 60h to obtain low-fat peanut polypeptide Liquid; add guava juice at the ratio of guava juice / low-fat peanut polypeptide liquid=1 / 9, mix, homogenize, sterilize (80℃, 15min), and package to obtain guava juice peanut polypeptide liquid beverage. The centrifuge is a three-phase centrifuge or a ...

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Abstract

The invention discloses a method for preparing a peanut polypeptide solution by utilizing peanut intrinsic protease to hydrolyze peanut protein, and belongs to the technical field of processing of plant protein and grease. The method comprises the following steps: soaking peanuts, removing peanut skin, removing mildewed and deteriorated peanut kernels, leaching and pulping to obtain peanut pulp; treating through a centrifugal machine to obtain peanut oil body enrichment and low fat peanut pulp; activating the peanut intrinsic protease in the low fat peanut pulp by carrying out acid treatment,and hydrolyzing low fat peanut pulp protein by using the activated peanut intrinsic protease to generate a low fat peanut polypeptide solution; blending and sterilizing to obtain a peanut polypeptidesolution beverage. No organic solvents or enzyme preparations are added, so that the method disclosed by the invention is a method with simple process, low processing cost and environment friendliness, and a peanut polypeptide solution product with low fat, nutrition, health and good functions can be obtained; a by-product peanut oil body enrichment also can be obtained while the peanut polypeptide solution is obtained; the peanut polypeptide solution is a novel peanut oil product and can be applied to soups, alternative dilute cream and the like.

Description

Technical field [0001] The invention relates to a method for preparing peanut polypeptide liquid by hydrolyzing peanut protein by using peanut inherent protease, and belongs to the technical field of processing plant protein and oil. Background technique [0002] Peptide production mainly includes two methods: synthesis and protein hydrolysis in vitro. The synthesis method includes three methods: chemical synthesis, recombinant technology synthesis, and enzymatic synthesis. Although the synthetic method can synthesize any active peptide according to people's wishes, it has problems such as high cost, many by-products and residues. In vitro protein hydrolysis methods include acid hydrolysis, alkali hydrolysis and enzymatic hydrolysis. The first two methods are simple and easy to operate, but may have harmful effects in nutrition and toxicology, so their application is limited to a certain extent. Enzymatic hydrolysis can perform targeted hydrolysis under certain conditions to p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14A23J3/34A23L2/02
CPCA23J1/14A23J3/346A23L2/02
Inventor 彭晓庆
Owner 南京爱美倍健生物科技有限公司
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