Preparation method of lactic acid bacteria powder for yoghourt production
A technology of lactic acid bacteria and bacteria powder, applied in the field of fermentation, can solve the problems of poor stability of direct-throwing starter, great damage to bacterial cells, low vitality, etc., so as to save the cost of culture medium and process time, and improve the number of viable bacteria and bacterial cells. the effect of vitality
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[0032] The present invention provides a kind of preparation method of the bacterium powder of lactic acid bacteria used for yoghurt production, and described method comprises:
[0033] 1) medium preparation step, wherein, prepare enrichment medium, described medium comprises by weight ratio:
[0034] 6%~12% skimmed milk powder,
[0035] 3% to 7% tomato juice,
[0036] 0.3%~0.9% yeast powder,
[0037] 3% to 7% wort,
[0038] 1% to 3% lactose,
[0039] 0.01%~0.05% dipotassium hydrogen phosphate,
[0040] 0.02%~0.06% potassium dihydrogen phosphate,
[0041] 0.05%~0.1% Tween,
[0042] 0.01-0.05% vitamins B1, B2 and / or B6 and
[0043] remaining water
[0044] 2) Sterilization step, wherein, the culture medium is sterilized, the sterilization condition is that the temperature is 85-90° C., the time is 60-90 minutes, and the pH value of the fermentation broth is adjusted to 5.8-90 minutes with sodium hydroxide lye 6.3;
[0045] 3) Inoculation step, wherein Streptococcus the...
Embodiment 1
[0055] The following media formulations were used:
[0056] Table 1 medium formula
[0057] raw material
Ratio (weight%)
skimmed milk powder
10％
tomato sauce
5％
0.5％
Wort
3％
1％
0.02％
Potassium dihydrogen phosphate
0.02％
twain
0.05％
Vitamin B1, B2, B6
0.01% each
The balance is water
add to 100%
[0058] 2) Sterilization step, wherein, the culture medium is sterilized, the sterilization condition is that the temperature is 85° C., and the time is 65 minutes;
[0059] 3) The inoculation step, wherein the Streptococcus thermophilus BNCC187957 (purchased from Beina Biology) activated by conventional methods is inoculated in the above-mentioned culture medium with a 3% inoculation amount, and 18% (w / v) hydrogen Sodium oxide lye adjusts the pH value of the fermentation broth to 5.8, the culture temperature is 33°C, the culture time ...
Embodiment 2
[0065] Adopt the same operation as embodiment 1, the difference is:
[0066] 1) The strain of Streptococcus thermophilus is BNCC340730 (purchased from Beina Biotech).
[0067] 2) Sterilization step, wherein, the culture medium is sterilized, the sterilization condition is that the temperature is 90° C., and the time is 60 minutes;
[0068] 3) an inoculation step, wherein, inoculate the Streptococcus thermophilus BNCC340730 activated by a conventional method in the above-mentioned medium with a 3% inoculation amount, and inoculate the fermentation broth with 18% (w / v) sodium hydroxide lye The pH value was adjusted to 5.8, the culture temperature was 33°C, the culture time was 15 hours, and cultured to the end of fermentation;
[0069] 4) Collect the bacteria by centrifugation, mix the centrifuged sediment (collected bacteria) and the protective agent in equal proportions, fully homogenize and freeze-dry directly, lower the temperature to -40°C within 50 minutes, and keep it wa...
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