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Apparatus for detecting mercury, lead and arsenic ions in water body based on quantum dot resonance energy transfer of intelligent equipment and method thereof

A technology of energy resonance transfer and intelligent equipment, which is applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of complex data processing and analysis, complex operation, and difficulty in on-site detection and analysis, and achieve simple operation, simple process, and work Small amount of effect

Active Publication Date: 2018-04-20
TAIYUAN UNIV OF TECH
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Problems solved by technology

[0005] In order to solve the existing detection devices for heavy metal mercury ions, lead ions and arsenic ions and the problems of complex operation, complex data processing and analysis, and difficulty in on-site detection and analysis, the present invention provides a quantum dot energy resonance transfer detection water body based on intelligent equipment Apparatus and method for neutralizing mercury, lead and arsenic ions

Method used

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  • Apparatus for detecting mercury, lead and arsenic ions in water body based on quantum dot resonance energy transfer of intelligent equipment and method thereof
  • Apparatus for detecting mercury, lead and arsenic ions in water body based on quantum dot resonance energy transfer of intelligent equipment and method thereof
  • Apparatus for detecting mercury, lead and arsenic ions in water body based on quantum dot resonance energy transfer of intelligent equipment and method thereof

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preparation example Construction

[0041] Preparation of glass fiber paper-based chips: glass fiber filter paper without background fluorescence was used as the glass fiber paper base, and multiple channels were constructed on the paper base: the hydrophobic channels in the non-reaction zone and the hydrophilic channels in the reaction zone were spaced apart, and the preparation of the hydrophobic channels The method is as follows: soak the glass fiber paper base in a mixture of 0.12 M methyltrichlorosilane MTS, toluene and hydrochloric acid, react in a fume hood for 3-3.5 hours, remove the glass fiber paper base after the reaction is complete, and dry it in the air Standby; the hydrophilic channel is a circular heavy metal detection reaction zone with an inner diameter of 2-6mm, and the glass fiber paper base in the reaction zone is not hydrophobicized;

[0042] B. Construction of the detection device: The glass fiber paper-based chip and the optical hardware device that provides uniform illumination for taking...

experiment example 1

[0044] Experimental Example 1: Pb 2+ , Hg 2+ and As 3+ Detection of single ions: detection of Pb using QD620-DNA2 fluorescent probe prepared at 32nM 2+ , 32nM prepared QD540-DNA1 fluorescent probe to detect Hg 2+ , 32nM prepared QD490-DNA3 fluorescent probe to detect As 3+ ; Use 10mM sodium dihydrogen phosphate / disodium hydrogen phosphate NaH2PO4 / Na2HPO4 to immobilize the above fluorescent probes on the 3 reaction areas of the glass fiber paper chip; 2+ , As 3+ or containing Pb at concentrations of 0-50 nM to be tested 2+ The aqueous solutions were respectively added to the above-mentioned glass fiber paper-based chip reaction zone channels, and maintained at 25 °C for 0.5 h, so that the Pb 2+ or Hg 2+ Induced DNA conformational changes that allow As 3+ Combined with the aptamer chain, the paired DNA chain is opened, and the reaction is completely carried out, and then the intelligent device collects the RGB picture information of multiple different reaction areas of t...

experiment example 2

[0045] Experimental Example 2: Pb 2+ , Hg 2+ and As 3+ Detection of mixed solution: use 10mM sodium dihydrogen phosphate / disodium hydrogen phosphate NaH2PO4 / Na2HPO4 to immobilize the fluorescent probes on the three reaction areas of the glass fiber paper chip; 2+ , Hg 2+ and As 3+ The mixed solution was added to the above-mentioned glass fiber paper-based chip reaction zone channel, and maintained at 25 °C for 0.5 h to make Hg 2+ Induction of conformational inversion of DNA1 to form T-Hg 2+ -T structure, Pb 2+ Induces a conformational change in DNA2 to form a G-quadruplex, As 3+ Combined with the aptamer chain, the paired DNA chain is opened and the reaction is completed. Then the smart device collects the RGB picture information of multiple different reaction areas of the glass fiber paper-based chip; the APP analysis program simultaneously obtains the fixed Pb 2+ ,Hg 2+ and As 3+ The R value, G value and B value corresponding to multiple pixels in the reaction area,...

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Abstract

The invention belongs to the technical field of heavy metal ion detection, and provides an apparatus for detecting mercury, lead and arsenic ions in a water body based on quantum dot resonance energytransfer of intelligent equipment and a method thereof, which can be used for solving the problems of complex detection apparatus for the current heavy metals such as mercury ion, lead ions and arsenic ions as well as operation, complex data processing analysis, and difficult on-site detection analysis. The apparatus is a minisize darkroom arranged below the intelligent equipment, a glass fiber paper-based chip is fixed at bottom in the minimized darkroom, reaction zones and non-reaction zones are arranged on the glass fiber paper-based chip at intervals, an optical hardware apparatus is arranged at the top of the glass fiber paper-based chip, and a photo-taking program and a heavy metal ion concentration value calculating program are arranged on the intelligent equipment. The apparatus and the method can simultaneously detect Pb<2+>, Hg<2+>, and As<3+>, cost is low, a process is simple, and is benefit for on-site detection and analysis of heavy metal concentration in the polluted water body, detection is convenient, fast and accurate, and the apparatus and the method are benefit for experiment detection examination and convenient for real-time detection of pollution condition in the water body in life.

Description

technical field [0001] The invention belongs to the technical field of heavy metal ion detection, and in particular relates to a device and method for detecting mercury, lead and arsenic ions in water by quantum dot energy resonance transfer based on intelligent equipment. Background technique [0002] Mercury ions, lead ions and arsenic ions are the three most common sources of heavy metal pollution, which are non-biodegradable and cumulative, and produce persistent toxicity. Human infection will cause irreversible damage, which is manifested in the hazards of the digestive system, nervous system and cardiovascular system. Therefore, the development of convenient, rapid and accurate detection of heavy metals such as lead and mercury has attracted more and more attention. traditional Hg 2+ , Pb 2+ and As 3+ The quantitative detection methods include: atomic absorption spectrometry (AAS), inductively coupled plasma mass spectrometry (ICP-MS); and a large number of new sen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 李晓春张玲玲张校亮于化忠
Owner TAIYUAN UNIV OF TECH
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