Method for detecting melamine by using nucleic acid sensitized rare earth luminescence sensor
A luminescence technology of melamine and rare earth, which is applied in the field of analytical chemistry, can solve the problems of time-consuming and labor-intensive processing, complicated operation, and heavy labor load, and achieve good practical value and the effect of sensitive analysis and detection methods
Inactive Publication Date: 2018-04-20
JILIN UNIV
View PDF5 Cites 5 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Although these methods have high precision, the equipment is expensive, the operation is complicated, and the sample pretreatment is time-consuming, labor-intensive, costly, and labor-intensive.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0026] (1) Construction of a nucleic acid molecular switch for hybridization of aptamers and complementary strands
[0027] Materials / Reagents: Melamine aptamers and complementary chains were purchased from Sangon Bioengineering Co., Ltd. (Shanghai, China).
[0028]Method: Centrifuge the purchased nucleic acid aptamer and complementary chain at -4°C, 2500 rpm for 5 min, prepare Tris-HCl buffer solution with pH 7.7, dilute the melamine aptamer and complementary chain to a concentration of 10 μM solution; add 75 μL of melamine aptamer working solution and 50 μL of complementary chain working solution into a 1.5 mL centrifuge tube, vortex the mixer and mix thoroughly, then put it in a 95°C water bath for 10 minutes, and heat it for 10 minutes at room temperature. Cool down for more than 30 minutes, and dilute 10 times with Tris-HCl buffer solution, and store in a refrigerator at 4°C overnight for use.
[0029] Results: The nucleic acid aptamer fully hybridized with the complemen...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a method for detecting melamine by using a nucleic acid sensitized rare earth luminescence sensor. The method is established by combining the characteristics of specific recognition and target binding of an aptamer and the luminous characteristic of single-stranded deoxyribonucleic acid (DNA)-sensitized Tb<3+>; when the melamine aptamer and a complementary strand thereof form a double helix, the sensitization of Tb<3+> fluorescence is weakened; when melamine exists, the aptamer and the melamine recognize and bind with each other so as to release the complementary strand, and the free complementary strand excite the fluorescence of the TB<3+> significantly; by measuring the changes of the fluorescence intensity of the system when the melamine is added or not added,the qualitative and quantitative detection of the melamine can be realized. The establishment of the method comprises the following steps: constructing a molecular switch of a nucleic acid biosensor;establishing a fluorescence method based on the rare earth sensitized luminescence and nucleic acid aptamer recognition for detecting the melamine; analyzing the selectivity of the melamine detected by the established nucleic acid aptamer sensor; applying the established nucleic acid aptamer fluorescence sensor to determine the melamine in milk.
Description
technical field [0001] A nucleic acid aptamer fluorescent sensor based on rare earth sensitized luminescence detects melamine, which belongs to the technical field of analytical chemistry. Background technique [0002] Melamine (1,3,5-triazine-2,4,6-triamine, C3H6N6) is an odorless, slightly bitter white crystal, slightly soluble in water, obtained by the polymerization of urea, mainly used for synthesis Melamine resin and the preparation of bleaching agents and herbicides. Because its nitrogen content is as high as 66%, it was illegally added to milk by unscrupulous merchants, causing a series of food safety problems. Because of the slow metabolism in the human body and easy accumulation, intake of melamine exceeding the safe limit will cause kidney stones or even renal failure. [0003] At present, the qualitative and quantitative analysis methods of melamine are mainly high-performance liquid chromatography, gas chromatography-mass spectrometry, and liquid chromatograph...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N21/64C12N15/115
CPCC12N15/115C12N2310/16G01N21/6428
Inventor 孙春燕苏瑞芳别佳昕乔尚娜马欣月孙蕊孙宏靖
Owner JILIN UNIV