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Method for detecting content of gamma-aminobutyric acid in livestock and poultry tissues by high performance binary liquid chromatography method

A liquid chromatography and aminobutyric acid technology is applied in the field of high-performance binary liquid chromatography to detect the content of γ-aminobutyric acid in livestock and poultry tissues, and can solve the problem of low accuracy of detection results and inability to directly obtain γ-aminobutyric acid content and other issues to achieve high accuracy

Inactive Publication Date: 2018-05-01
GUANGZHOU AONONG BIOTECH +2
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  • Description
  • Claims
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Problems solved by technology

The accuracy of the detection results of this detection method is low, and it is impossible to directly obtain the content of gamma-aminobutyric acid in meat, the main product of livestock and poultry

Method used

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  • Method for detecting content of gamma-aminobutyric acid in livestock and poultry tissues by high performance binary liquid chromatography method
  • Method for detecting content of gamma-aminobutyric acid in livestock and poultry tissues by high performance binary liquid chromatography method

Examples

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Effect test

Embodiment 1

[0045] When researchers were studying the effect of GABA on the production performance of pigs under heat stress, they detected the residual GABA in pork tissue according to the detection method of this example.

[0046] 1. Instruments and reagents

[0047] 1. Instrument: high performance liquid chromatography system, including two pumps, ultraviolet detector, automatic gradient controller, temperature controller and column thermostat; sampling valve; chromatography workstation, amino acid detection column.

[0048] 2. Standard substance of γ-aminobutyric acid, phenyl isothiocyanate (derivative agent); acetonitrile, methanol (chromatographically pure), sodium acetate (analytical pure), first-grade water.

[0049] 2. Chromatographic conditions

[0050] Chromatographic column: special column for amino acid;

[0051] Mobile phase: mobile phase A is acetonitrile: 70mmol / L acetate buffer (PH=6.5): ethylenediaminetetraacetic acid=25:975:0.25 (mass ratio);

[0052] Mobile phase B ...

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Abstract

The invention provides a method for detecting the content of gamma-aminobutyric acid in livestock and poultry tissues by a high performance binary liquid chromatography method and relates to the technical field of detecting content of gamma-aminobutyric acid. According to the method, livestock and poultry tissues are weighed; liquid nitrogen is added; grinding is performed; the operation is repeated in such a way many times until the livestock and poultry tissues are completely crushed; sample diluent is added; high-speed centrifugation is performed; supernatant fluid is taken to pass througha microporous filtering film; filter liquid is frozen and dried; a regulating solution is added; freeze drying is performed; then, derivatization agent is added; freeze drying is performed after the room temperature derivatization; a sample is obtained. The sample is dissolved again by the sample diluent to obtain a test specimen; the test specimen and a standard sample are injected into a high performance binary liquid chromatography instrument for analysis; a sample and standard sample chromatogram is obtained; the content of gamma-aminobutyric acid in the sample is calculated according to the chromatogram. The detection method has the advantages that phenyl isothiocyanate is used as a derivating agent to react with amino acid in the livestock and poultry tissues; derivatives are detected by an ultraviolet detector after binary high performance liquid phase separation; the residue of the gamma-aminobutyric acid in livestock and poultry tissues can be directly detected; the accuracy of the detection result is high.

Description

technical field [0001] The invention relates to the technical field of gamma-aminobutyric acid content detection, and in particular to a method for detecting gamma-aminobutyric acid content in livestock and poultry tissues by high-efficiency binary liquid chromatography. Background technique [0002] γ-aminobutyric acid (γ-Aminobutyric acid, GABA) is also known as aminobutyric acid and pipecolic acid, and its molecular formula is C 4 h 9 NO 2 , the molecular weight is 130.12. γ-aminobutyric acid is a non-protein amino acid widely distributed in animals and plants. At present, γ-aminobutyric acid has been deeply studied in medicine as an important inhibitory neurotransmitter, which has biological functions such as enhancing brain vitality, calming the nerves, regulating hormone secretion, improving lipid metabolism, and lowering blood pressure. Based on the above functions of gamma-aminobutyric acid, it is widely used in the livestock and poultry industry. Gamma-aminobuty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/74G01N30/8634G01N2030/067G01N2030/146
Inventor 叶丽杨伟春黎文彬张芳平吴有林周通闫卫飞刘国梁
Owner GUANGZHOU AONONG BIOTECH
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