A kind of bacillus that antagonizes mold and its antibacterial application
A technology of bacillus and mold, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as economic loss, stagnation, and affecting fruit appearance and quality
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Embodiment 1
[0089] Embodiment 1 Bacillus and Penicillium growth rate comparison of antagonistic mold of the present invention
[0090] The mold-antagonistic Bacillus and Penicillium of the present invention were respectively inoculated in the common culture solution, and the growth curves of the two were measured.
[0091] Adopt spectrophotometry to measure the growth curve of the bacillus of antagonistic mold of the present invention such as Figure 6 Shown in line A. It can be seen that the mold-antagonizing bacillus of the present invention reaches the logarithmic phase and the stable phase in a short time, 1 h and 6 h, respectively.
[0092]The growth curve of Penicillium was determined by dry weight method. Figure 6 In line B, it is found that the time to reach the logarithmic phase and the stable phase is very long, respectively 20h and 60h, which is 10 times as long as that of the mold-antagonizing Bacillus of the present invention.
[0093] Comparison shows that the mold-antag...
Embodiment 2
[0094] Embodiment 2 inhibits the colonization growth of Penicillium rodian navel orange with the bacillus of the present invention which antagonizes mold
[0095] Preparation of the mold-antagonizing bacillus suspension of the present invention: insert the mold-antagonizing bacillus of the present invention into the LB culture solution, shake and culture at 37°C for 8-15 hours, and obtain the mold-antagonizing bacillus suspension of the present invention .
[0096] Preparation of Penicillium spore suspension: Inoculate Penicillium on several potato slant mediums by streaking on a slant, and culture at a constant temperature of 28°C for 7 days. Add a total of 2 mL of sterile saline to each tube 3 times, collect and wash the spore suspension to obtain the Penicillium spore suspension.
[0097] Use sterile scissors to stab a square wound with a side length of 4 mm and a depth of 3 mm on the waist surface of commercially available Luodian navel orange, soak the wound in the bacte...
Embodiment 3
[0099] Embodiment 3 inhibits the colonization growth of Daxian navel orange penicillium with the bacillus that antagonizes mold of the present invention
[0100] Preparation of the mold-antagonizing bacillus suspension of the present invention: insert the mold-antagonizing bacillus of the present invention into the LB culture solution, shake and culture at 37°C for 8-15 hours, and obtain the mold-antagonizing bacillus suspension of the present invention .
[0101] Preparation of Penicillium spore suspension: Inoculate Penicillium on several potato slant mediums by streaking on a slant, and culture at a constant temperature of 28°C for 7 days. Add a total of 2 mL of sterile saline to each tube 3 times, collect and wash the spore suspension to obtain the Penicillium spore suspension.
[0102] Use sterile scissors to stab a square wound with a side length of 4 mm and a depth of 3 mm on the waist surface of the commercially available Daxian navel orange, soak the wound in the bac...
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