A kind of bacillus that antagonizes mold and its antibacterial application

A technology of bacillus and mold, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as economic loss, stagnation, and affecting fruit appearance and quality

Active Publication Date: 2020-10-27
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Plant fungal disease is an important problem that causes agricultural production and food safety. The invasion of pathogenic microorganisms will cause slow growth and stagnation of plants, affect the appearance and quality of fruits, and even cause devastating crop death, grain failure, and huge economic losses ( Xie Jing. Screening, identification and research on the mechanism of antagonistic microorganisms against plant pathogenic bacteria [D]: Sichuan University, 2004)
For a long time, the prevention and control of plant diseases has mainly relied on the use of chemical pesticides and fertilizers. Although the effect is remarkable, it has caused soil and groundwater pollution, which is a more serious ecological problem.

Method used

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  • A kind of bacillus that antagonizes mold and its antibacterial application
  • A kind of bacillus that antagonizes mold and its antibacterial application
  • A kind of bacillus that antagonizes mold and its antibacterial application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Embodiment 1 Bacillus and Penicillium growth rate comparison of antagonistic mold of the present invention

[0090] The mold-antagonistic Bacillus and Penicillium of the present invention were respectively inoculated in the common culture solution, and the growth curves of the two were measured.

[0091] Adopt spectrophotometry to measure the growth curve of the bacillus of antagonistic mold of the present invention such as Figure 6 Shown in line A. It can be seen that the mold-antagonizing bacillus of the present invention reaches the logarithmic phase and the stable phase in a short time, 1 h and 6 h, respectively.

[0092]The growth curve of Penicillium was determined by dry weight method. Figure 6 In line B, it is found that the time to reach the logarithmic phase and the stable phase is very long, respectively 20h and 60h, which is 10 times as long as that of the mold-antagonizing Bacillus of the present invention.

[0093] Comparison shows that the mold-antag...

Embodiment 2

[0094] Embodiment 2 inhibits the colonization growth of Penicillium rodian navel orange with the bacillus of the present invention which antagonizes mold

[0095] Preparation of the mold-antagonizing bacillus suspension of the present invention: insert the mold-antagonizing bacillus of the present invention into the LB culture solution, shake and culture at 37°C for 8-15 hours, and obtain the mold-antagonizing bacillus suspension of the present invention .

[0096] Preparation of Penicillium spore suspension: Inoculate Penicillium on several potato slant mediums by streaking on a slant, and culture at a constant temperature of 28°C for 7 days. Add a total of 2 mL of sterile saline to each tube 3 times, collect and wash the spore suspension to obtain the Penicillium spore suspension.

[0097] Use sterile scissors to stab a square wound with a side length of 4 mm and a depth of 3 mm on the waist surface of commercially available Luodian navel orange, soak the wound in the bacte...

Embodiment 3

[0099] Embodiment 3 inhibits the colonization growth of Daxian navel orange penicillium with the bacillus that antagonizes mold of the present invention

[0100] Preparation of the mold-antagonizing bacillus suspension of the present invention: insert the mold-antagonizing bacillus of the present invention into the LB culture solution, shake and culture at 37°C for 8-15 hours, and obtain the mold-antagonizing bacillus suspension of the present invention .

[0101] Preparation of Penicillium spore suspension: Inoculate Penicillium on several potato slant mediums by streaking on a slant, and culture at a constant temperature of 28°C for 7 days. Add a total of 2 mL of sterile saline to each tube 3 times, collect and wash the spore suspension to obtain the Penicillium spore suspension.

[0102] Use sterile scissors to stab a square wound with a side length of 4 mm and a depth of 3 mm on the waist surface of the commercially available Daxian navel orange, soak the wound in the bac...

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Abstract

The invention discloses a bacillus antagonizing mold and its antibacterial application, belonging to the technical field of microorganism prevention and control. The mold-antagonizing bacillus provided by the invention is a bacillus strain with broad-spectrum antibacterial ability. It was preserved in the China Center for Type Culture Collection, and sequence comparison analysis found that the strain was 100% related to the known Bacillus amyloliquefaciens Holycan 1 (Bacillus amyloliquefaciens Holycan 1, KM54172), and it was determined that it belonged to Bacillus amyloliquefaciens (Bacillus amyloliquefaciens). The mold-antagonistic bacillus provided by the invention has a very short time to reach the stable period, which is conducive to its rapid colonization and occupation of ecological niches, and competition with other molds for nutrients and living space. Therefore, the Penicillium provided by the invention antagonizes Bacillus amyloliquefaciens, has a good inhibitory effect on common crop disease fungi, shows a broad antibacterial spectrum, has a large biocontrol potential, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganism prevention and control, and relates to a bacillus, in particular to an antagonizing mold bacillus and its antibacterial application. Background technique [0002] Mold is ubiquitous and highly resistant to stress due to the formation of spores. Since mold is a chemoheterotrophic fungus, it can borrow organic matter to live. Therefore, wherever there is organic matter, if the temperature and humidity are appropriate, it may cause mold pollution or infection, which will bring a lot of trouble to human society. For example, in the storage of fruits, vegetables, grain and feed, it is easy to be polluted by mold, causing economic losses and even poisoning of humans and animals; Affect the growth of plants, resulting in reduced production and so on. The prevention and treatment of mold pollution is a protracted battle that we have had for a long time and may continue for a long time. [0003] B...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A23B7/155A23B9/28C12R1/07
CPCA01N63/00A23B7/155A23B9/28C12N1/205C12R2001/07
Inventor 杨水云吴丹苏婷婷李少锋赵浩淼
Owner XI AN JIAOTONG UNIV
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