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Serotonin-magnetic particle compound and method for enriching sialylated glycoprotein

A technology of sialylated sugar and magnetic particles, which is applied in the field of sialylated glycoprotein detection, can solve the problems of long time-consuming enrichment process, high cost, harsh reaction conditions, etc. good stability effect

Active Publication Date: 2018-05-04
陕西中药研究所
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Problems solved by technology

However, these methods have their own shortcomings, such as low specificity and high cost of enrichment using lectins, while hydrophilic chromatography, strong cation exchange chromatography, titanium dioxide chromatography, and lignocellulosic chromatography are affected by phosphorylated proteins. Time-consuming and harsh reaction conditions
At present, sialoglycoprotein / glycopeptide is enriched by using serotonin as a ligand. The main problem is that it is difficult to quickly separate and obtain bound sialoglycoprotein / glycopeptide after ligand recognition and binding.
[0004] At present, there is no report on the preparation of magnetic particles coupled with serotonin.

Method used

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  • Serotonin-magnetic particle compound and method for enriching sialylated glycoprotein
  • Serotonin-magnetic particle compound and method for enriching sialylated glycoprotein
  • Serotonin-magnetic particle compound and method for enriching sialylated glycoprotein

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Embodiment Construction

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0034] 1. Experimental part

[0035] 1. Reagents and instruments

[0036] 3-glycidyl propyl ether trimethoxysilane, referred to as GPTS (C3), 3-glycidyl butyl ether trimethoxysilane, referred to as GPTS (C4), 3-glycidyl pentyl ether trimethoxysilane, referred to as GPTS (C5 ), FeCl 3 ·6H 2 O, FeCl 2 4H 2 O, NaOH, glacial acetic acid, ethanolamine, and potassium bromide (KBr) were purchased from Sigma-Aldrich, USA; acrylamide and N,N-methylene acrylamide were purchased from Amresco; sodium chloride, trisodium phosphate, and serotonin amine Hydrochloride (serotonin hydrochloride), sodium bicarbonate and other commonly used reagents are domestic analytical reagents; magnetic separator: Xi'an Jin Magnetic Nano Biotechnology Co., Ltd.; ZHWY-2101C constant temperature air oscillator: Shanghai Zhicheng Analytical Instruments Manufacturing Co., ...

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Abstract

The invention provides a serotonin-magnetic particle compound and a method for enriching sialylated glycoprotein. The compound consists of magnetic particles that serotonin is immobilized on surfaces.As epoxy modified magnetic particles are adopted as a carrier for immobilizing serotonin, under a slight alkali condition, serotonin is covalently coupled with the surfaces of epoxy magnetic particles through open loop reactions of amino and epoxy groups. Through orientated response of magnetic particles to a magnetic field, and the specific recognition of serotonin upon sialic acid, an ialylatedglycoprotein enriching method which is simple and convenient to operate and good in stability is established. Sialylated glycoprotein of a complex protein sample is enriched, and relatively good results can be made.

Description

technical field [0001] The invention relates to the detection of sialylated glycoproteins, in particular to a serotonin-magnetic microparticle complex and a method for enriching sialylated glycoproteins using the complex. Background technique [0002] Sialic acid is a general term for a class of carboxyl-containing nine-carbon monosaccharide compounds and their derivatives. Protein sialylation modification is one of the important post-translational modifications of proteins, which has a very important impact on the structure and function of proteins. It plays an important biological role, such as recognition between virus and cells, signal transduction, immune response and interaction between cells. In addition, abnormal sialylation in organisms is closely related to the occurrence and development of many tumors. Due to the low abundance of sialoglycoprotein in complex samples and the heterogeneity of microstructure, especially the lower content of sialoglycopeptides after e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/34G01N1/28
CPCG01N1/28G01N1/34G01N21/6486
Inventor 李铮王喜龙肖志强秦秋红苏建英麻纪斌高湘马恬然张志伟
Owner 陕西中药研究所
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