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Root induction method in kiwi fruit tissue culture

A tissue culture and kiwifruit technology is applied in the field of plant tissue culture to achieve the effects of high light utilization rate, developed root system and high survival rate

Inactive Publication Date: 2018-05-08
宝鸡松良农业科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method can only get 3-5 taproots when rooting, and there is no root hair, such as figure 2 As shown, the seedlings need to be hardened and cultivated in the later stage

Method used

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  • Root induction method in kiwi fruit tissue culture
  • Root induction method in kiwi fruit tissue culture
  • Root induction method in kiwi fruit tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The method of the present invention is used to carry out tissue culture with Actinidia xuxiang leaves as tissue culture material.

[0019] 1. Select the emerald green, thick leaves on the strong kiwifruit plant, which are relatively close to the stem tip, as the explant material, and wash them with clean water;

[0020] Sterilize with 0.1% mercuric chloride solution for 10 minutes, then sterilize with 75% alcohol for 5 minutes, and wash with sterile water for 3-5 times;

[0021] Cut the treated leaves into squares with a side length of 1.5 cm, put them on the induction medium,

[0022] Light 800LX, 10h per day, temperature 25±2°C, humidity 70%, culture for about 50 days to form kiwifruit callus.

[0023] The induction medium is: MS medium + sucrose 20g / L + agar 6g / L + 6-BA0.8mg / L + NAA (naphthalene acetic acid) 0.2mg / L + 2,4-D (2,4-dichlorophenoxy Acetic acid) 0.5mg / L culture medium;

[0024] 2. Inducing Cluster Buds

[0025] Divide the callus into small pieces with...

Embodiment 2

[0030] The leaves of Actinidia kiwifruit were used as tissue culture materials, and different methods were used for tissue culture.

[0031] Experimental group: use the method for inducing rooting in Step 4 of Example 1 of the present invention;

[0032] Control group 1: the medium used in the step of inducing rooting is: 0.7mg L -1 IBA, 30mg·L -1 Sucrose, 7mg·L -1 1 / 2 MS medium of agar, the others are the same as the experimental group.

[0033] Control group 2: the medium used in the step of inducing rooting is: in 1 / 2MS+NAA0.2mg L -1 , and the others are the same as the experimental group.

[0034] Control group 3: the light time used in the rooting induction step is 10h, the temperature is 25±2°C, the humidity is 85%, and the others are the same as the experimental group.

[0035] Control group 4: the carbon dioxide concentration used in the rooting induction step is 1200ppm, and the others are the same as the experimental group.

[0036]

Embodiment 3

[0038] A special rooting device for tissue culture, such as Figure 3 to Figure 5 As shown, it includes: a box body 1, a box cover 2 and a root bed 3, the box cover 2 is connected with the box body 1 through a hinge 26, and can be turned over relative to the box body 1; Composed of partitions, the root bed 3 has several mutually independent well-shaped root bed areas 30 for accommodating medium, the bottom surface of the box cover 2 is provided with LED lights 20, the box body 1 A number of ventilation holes 10 are opened on the side wall, and the location of the ventilation holes 10 is higher than the top surface of the root bed 3 . The roots grown by the kiwifruit seedlings can be separated by the root bed 3, which avoids the entanglement of their root systems after the cultivation of the seedlings is completed, and it takes time and effort to separate them and easily damages the root system.

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Abstract

The invention relates to a root induction method in a kiwi fruit tissue culture. The method comprises the following steps: inserting a plant, with more than three leaves, which is more than 3 cm aftermultiplication into a substrate, wherein the substrate comprises MS and vermiculite, the illumination time lasts for 16 h, 1500 to 2000 LX, the temperature is 25+ / -2 DEG C, the concentration of carbon dioxde is 950 ppm, the humidity is 80%, and the cleanliness of a culture room is higher than level 1000; and forming a complete kiwi fruit plant with developed root system after culture for 20 days.According to the method, MS and vermiculite are taken as the culture medium, the growth environment of kiwi fruit is simulated preferably, the illumination time and strength are strictly controlled,simultaneously, the humidity and the concentration of carbon dioxide are controlled, and a good growth environment is provided for rapid rooting of plants after kiwi fruit multiplication. Through thetechnical scheme, the problem that the rooting quality of woody tissue culture seedlings is low is solved, the root system of the tissue culture seedlings is developed and complete, and the tissue culture seedlings are high in survival rate and free of seedling hardening.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing rooting in kiwi fruit tissue culture. Background technique [0002] Kiwifruit (scientific name: Actinidia chinensis Planch) is a perennial woody vine. The texture of kiwifruit is soft and the taste is sweet and sour. The flavor is described as a trio of strawberry, banana, and pineapple. In addition to organic substances such as kiwifruit, proteolytic enzymes, tannin pectin and sugars, as well as trace elements such as calcium, potassium, selenium, zinc, germanium, and 17 kinds of amino acids needed by the human body, kiwifruit also contains rich vitamin C, grape Acid, fructose, citric acid, malic acid, fat. [0003] Traditional kiwifruit propagation methods include sowing, cutting, layering and grafting. These methods are relatively inefficient. At present, there are already many methods that use tissue culture to breed kiwifruit. For exampl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 蔡小军蔡军利
Owner 宝鸡松良农业科技有限公司