High-intensity biological ink material for 3D printing

A technology of bio-ink and 3D printing, which is applied in additive processing, medical science, prosthesis, etc., can solve the problems of insufficient strength, soft tissue bionic printing, etc., achieve good biocompatibility and biodegradability, and improve hydrogel Strength, effect of promoting adhesion

Inactive Publication Date: 2018-05-08
REGENOVO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a 3D printing high-strength bio-ink material for the lack of strength of the ex

Method used

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  • High-intensity biological ink material for 3D printing
  • High-intensity biological ink material for 3D printing
  • High-intensity biological ink material for 3D printing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) adding the gelatin solution to the alginic acid aqueous solution with a mass concentration of 20%, so that the mass content of the gelatin is 2%;

[0031] 2) The silk protein solution is added to the glycerol solution with a concentration of 200 mg / ml, so that the mass fraction of silk protein in the mixed solution is 30%, and mixed evenly.

[0032] 3) Add the solution in step 1 above to the solution in step 2, and mix evenly at 80° C. for 10 minutes to obtain a bio-ink.

[0033] 4) The model used is Regenovo – WS bioprinter, put the above bio-ink into the printing syringe, select a needle with a diameter of 0.21mm, print air pressure of 0.40MPa, print speed of 5mm / s, set the temperature of the syringe to 40°C, and the temperature of the platform to 40°C , using air pressure to squeeze the hydrogel onto the grid support with a three-dimensional deposition size of 15*15*3mm, a square filling shape, and a filling angle of 90° on the platform.

[0034] 5) Place the s...

Embodiment 2

[0040] 1) Prepare an alginic acid solution with a mass concentration of 10%, measure 10ml of the above-mentioned alginic acid solution, add it to 5ml of 400mg / ml gelatin solution and mix evenly at 40°C.

[0041] 2) Prepare a xylitol solution with a concentration of 1 g / ml, pipette 5 ml of the above xylitol solution and add it to the silk protein solution, so that the silk protein mass fraction in the mixed solution is 20%, and make it evenly mixed.

[0042] 3) Add the mixed solution of sodium alginate and gelatin in step 1 to the solution of xylitol and silk protein in step 2 and mix evenly at 40°C to obtain a hydrogel.

[0043] 4) The model used is Regenovo3D Bioprinter TM For a bioprinter, put the above-mentioned hydrogel in the printing syringe, select a needle with a diameter of 0.41mm, print air pressure at 0.1MPa, print speed at 10mm / s, set the temperature of the syringe at 40°C, and the temperature of the platform at -5°C , using air pressure to squeeze the hydrogel on...

Embodiment 3

[0046] 1) Prepare an alginic acid solution with a mass concentration of 5%, measure 10ml of the above-mentioned alginic acid solution and add it to 5ml of 400mg / ml gelatin solution, and add 5ml of hyaluronic acid with a mass fraction of 2% and 5ml with a concentration of 1mg / ml of collagen solution, mix evenly at 40°C.

[0047] 2) Prepare a propylene glycol solution with a concentration of 1 g / ml, pipette 5 ml of the above propylene glycol solution and add it to the silk protein solution, so that the mass fraction of silk protein in the mixed solution is 15%, and make it evenly mixed.

[0048] 3) Add the mixed solution of alginic acid and gelatin in the above step 1 to the propylene glycol and silk protein in step 2, and mix uniformly at 40° C. to obtain a hydrogel.

[0049] 4) The model used is Regenovo –Pro bioprinter, put the above hydrogel in the printing syringe, select a needle with a diameter of 0.34mm, print air pressure at 0.15MPa, print speed at 10mm / s, set the te...

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Abstract

The invention discloses a high-intensity biological ink material for 3D printing. According to the high-intensity biological ink material for 3D printing, a double-crosslinking network structure is formed by using fibroin for forming a beta folding structure and alginic acid for ionic crosslinking, the thermosensitive quick gelatinizing performance of gelatin is used at the same time, and a good forming effect is achieved. According to the high-intensity biological ink material for 3D printing, the mechanical strength of biological ink can be controlled, and the strength of hydrogel is remarkably improved. Aiming at the application characteristics of different organizations, a series of biological ink with different strengths can be prepared through matching of different materials, the customization design of 3D printing can be achieved for a patient, and the material is more suitable for clinical application.

Description

technical field [0001] The invention belongs to the technical field of biomedical polymer materials, in particular to a bio-ink used for 3D printing of bio-stents, tissues and organs. Background technique [0002] 3D bioprinting is based on the process of 3D printing biological materials (biological factors, cells and biocompatible materials). Among them, the printing material is often called bioink, a kind of based on cell-compatible hydrogel. [0003] Existing bioinks can be divided into synthetic hydrogels and natural hydrogels according to their sources: (1) synthetic hydrogels such as polyacrylamide (PAAM), polyvinyl alcohol (PVA), polyethylene glycol ( PEG), Planic, etc. are used as printing inks, but the biocompatibility needs to be further improved by chemical modification, so the safety is still carefully considered; (2) natural hydrogels such as collagen, fibrin, silk protein, Gelatin, agarose, sodium alginate, etc. have good biocompatibility and gel structure an...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/52C08J3/075C08L5/04C08L5/08C08L89/00B33Y10/00B33Y70/00
CPCA61L27/20A61L27/222A61L27/227A61L27/52B33Y10/00B33Y70/00C08J3/075C08J2305/04C08J2405/08C08J2489/00C08L89/00C08L5/04
Inventor 董世磊石然周永勇闵敏欧阳杨王玲徐铭恩
Owner REGENOVO BIOTECH
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