Zymogen fluid, product containing zymogen fluid and applied to lactation sows, as well as preparation method and application of product
A technology for fermenting bacteria and fermented feed, which is applied in the field of microbiology and feed processing, can solve the problems of complex preparation methods of fermented feed additives and difficulty in large-scale production, and achieve the promotion of intestinal health, low production cost, enhanced immunity and disease resistance force effect
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[0036] The present invention also provides a preparation method of the above-mentioned fermented feed additive, comprising:
[0037] The matrix and the fermented bacteria liquid are respectively prepared according to the formula quantity, the prepared fermented bacteria liquid is inoculated in the matrix, mixed evenly and fermented to obtain the fermented feed additive.
[0038] Wherein, the fermentation method is: inoculate the fermented bacteria liquid in the substrate, mix evenly, the inoculum amount of the fermented bacteria liquid in the substrate is 5-8% (v / w); the ratio of material to water is 1:1 by weight to volume -1:0.4; temperature, 35-40°C; fermentation time, 90-120h.
[0039] The preparation process of the fermented feed additive provided by the invention is simple, the fermentation process and the parameters of the fermentation process have been tested and optimized, the method is simple and convenient, and is easy for various farmers and feed production enterpr...
Embodiment 1
[0057] 1) Preparation of fermented bacteria liquid: take each microbial seed liquid prepared above, 20 ml of Clostridium butyricum bacterial liquid, 20 ml of Lactobacillus delbrueckii bacterial liquid, 20 ml of Aspergillus oryzae bacterial liquid, 60 ml in total, and mix evenly.
[0058] 2) Substrate composition: corn flour 520g, bran 145g, molasses 70g, soybean meal 70g, rapeseed meal 60g, peanut meal 50g, wheat protein powder 40g, soybean protein concentrate 40g, xylooligosaccharide 5g, salt 10g, a total of 1000g, each Mix ingredients and stir well.
[0059] 3) Take 60ml of the above-mentioned fermented bacteria liquid, inoculate it into 1000g of substrate, add 600ml of water, and mix well; place it in a sterile airtight fermenter with a fermentation temperature of 37°C and a fermentation time of 100h. The concentration of Clostridium butyricum in the fermentation material was 7.13log CFU / g; the concentration of Lactobacillus delbrueckii was 7.44log CFU / g; the concentration ...
Embodiment 2
[0062] 1) Preparation of fermented bacteria liquid: take each microbial seed liquid prepared above, 20 ml of Clostridium butyricum bacterial liquid, 30 ml of Lactobacillus delbrueckii bacterial liquid, 20 ml of Aspergillus oryzae bacterial liquid, 70 ml in total, and mix evenly.
[0063] 2) Matrix composition: corn flour 545g, bran 110g, molasses 80g, soybean meal 80g, rapeseed meal 50g, peanut meal 60g, wheat gluten powder 30g, soybean protein concentrate 30g, xylooligosaccharide 5g, salt 10g, total 1000g, each Mix ingredients and stir well.
[0064] 3) Take 70ml of the above-mentioned fermented bacteria liquid, inoculate it into 1000g of substrate, add 600ml of water, and mix well; place it in a sterile airtight fermenter with a fermentation temperature of 37°C and a fermentation time of 100h. The concentration of Clostridium butyricum in the fermentation material was determined to be 7.01log CFU / g; the concentration of Lactobacillus delbrueckii was 7.21log CFU / g; the concen...
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