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Camellia oleifera meal polypeptide, preparation method and effect of promoting estrus and conception in anestrous cows

A technology of camellia oleifera meal polypeptide and cow, which is applied in the field of animal husbandry, can solve the problems such as the in-depth development report of hydrolyzed polypeptide of camellia oleifera meal, and achieves the effect of realizing waste reuse, simple preparation method and increasing economic added value.

Active Publication Date: 2018-11-27
CP UNITED ANIMAL PHARMA TECH JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the in-depth development of hydrolyzed polypeptides of small fruit tea meal

Method used

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  • Camellia oleifera meal polypeptide, preparation method and effect of promoting estrus and conception in anestrous cows
  • Camellia oleifera meal polypeptide, preparation method and effect of promoting estrus and conception in anestrous cows
  • Camellia oleifera meal polypeptide, preparation method and effect of promoting estrus and conception in anestrous cows

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preparation of camellia oleifera meal polypeptide 1 by trypsin

[0047] Including the following steps:

[0048] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water to soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 150g / L;

[0049] Step S2, adding trypsin for enzymatic hydrolysis (the added weight is 3.0g / 100g based on the weight of meal powder), the enzymatic hydrolysis conditions are: pH value, 8.0; enzymatic hydrolysis temperature, 45°C; enzymatic hydrolysis time, 11 hours; end of enzymatic hydrolysis Afterwards, heat treatment at 90°C for 10 minutes to inactivate trypsin, cool to room temperature, let stand to take the supernatant;

[0050] Step S3, filtering the supernatant with absorbent cotton for 3 times, collecting and merging the filtrate, and freeze-drying to obtain a crude freeze-dried powder;

[0051] Step S4, dissolve the crude freeze-dried powder with ult...

Embodiment 2

[0052] Example 2: Preparation of camellia oleifera meal polypeptide 2 by trypsin

[0053] Including the following steps:

[0054] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water to soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 130g / L;

[0055] Step S2, adding trypsin for enzymatic hydrolysis (the added weight is 2.5g / 100g based on the weight of meal powder), the enzymatic hydrolysis conditions are: pH value, 7.8; enzymatic hydrolysis temperature, 43°C; enzymatic hydrolysis time, 12 hours; end of enzymatic hydrolysis Afterwards, heat treatment at 90°C for 10 minutes to inactivate trypsin, cool to room temperature, let stand to take the supernatant;

[0056] Step S3, filtering the supernatant with absorbent cotton for 3 times, collecting and merging the filtrate, and freeze-drying to obtain a crude freeze-dried powder;

[0057] Step S4, the crude freeze-dried powder is dissolved in u...

Embodiment 3

[0058] Example 3: Preparation of camellia oleifera meal polypeptide 3 by trypsin

[0059] Including the following steps:

[0060]Step S1, pulverize the camellia oleifera meal to 80-100 mesh, add water to soak overnight, and finally prepare a meal powder suspension, and the mass concentration of the meal meal suspension is 170 g / L;

[0061] Step S2, add trypsin for enzymolysis (the added weight is 3.5g / 100g based on the weight of meal meal), and the enzymolysis conditions are: pH value, 8.2; enzymolysis temperature, 47°C; enzymolysis time, 10 hours; enzymolysis end Then, the trypsin was inactivated by high temperature treatment at 90°C for 10 minutes, cooled to room temperature, and the supernatant was taken by standing;

[0062] Step S3, the supernatant is filtered 3 times with absorbent cotton, the combined filtrate is collected, and freeze-dried to obtain crude freeze-dried powder;

[0063] In step S4, the crude freeze-dried powder is dissolved in ultrapure water, and the ...

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Abstract

The invention discloses camellia meal polypeptides, a preparation method and application in promoting oestrus and conception of anestrus cows. The hydrolyzed polypeptides of camellia meal have variousactivities, and the activities of the hydrolyzed polypeptides are affected by proteolytic sites in the camellia meal: the polypeptide which is produced by hydrolysis of trypsin and has a molecular weight of (5,000 mu, 3,000 mu) has the effect of improving the oxidation resistance performance of bovine cryopreserved semen, and can be used as a bovine semen cryoprotective agent to reduce oxidativeinjury; the polypeptide which is produced by hydrolysis of alkaline protease and has a molecular weight of (5,000 mu, 3,000 mu) can promote the oestrus and the conception of the anestrus cows. Furthermore, the preparation method of the hydrolyzed polypeptides is simple, and only simple smashing, enzymolysis, ultrafiltration and lyophilization are used. The steps all can realize mass production ina factory and are easy to implement. According to the camellia meal polypeptides, the preparation method and the application in promoting the oestrus and the conception of the anestrus cows, waste reuse of small-fruit camellia meal is realized, and the economical additional value of the small-fruit camellia meal can be increased.

Description

technical field [0001] The invention belongs to the field of animal husbandry, and relates to camellia oleifera meal polypeptide, a preparation method and the function of promoting estrus and conception of anestrous cows. Background technique [0002] Camellia meiocarpa Hu. is the main species of Camellia oleifera (Camellia oleifera Abel.) in terms of cultivation area and annual output in my country. Hunan, Guizhou and other places. Small fruit camellia is also known as Jiangxizi, small tea, chicken heart, small leaf camellia, sheep excrement and so on. The fruit is small, the leaves are small, the buds are small, and the buds and bracts are not hairy. [0003] Camellia oleifera meal is a by-product obtained after extracting oil from Camellia oleifera. Although tea farms have detoxified these camellia leaves and used them as animal feed, due to the immature technology, most of the camellia leaves are still thrown away as waste, or used as fertilizer for tea farms after com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P15/08A23K50/10A23K20/147
CPCA23K20/147A23K50/10A61K38/00C07K1/34C12P21/06
Inventor 盛蓓蓓李沛王崇智
Owner CP UNITED ANIMAL PHARMA TECH JIANGSU
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