Puerarin and scutellarin lipid nanoparticle ophthalmic preparation and preparation method thereof

Abstract

The invention discloses a puerarin and scutellarin lipid nanoparticle ophthalmic preparation and a preparation method thereof. The preparation method comprises the steps as follows: (1) a lipid material, namely, scutellarin, is taken and dissolved in ethanol, a first oil phase is obtained, and distilled water serves as a first water phase; (2) lecithin and cholesterol are taken, a methanol and ethanol mixed solution is added, ultrasonic dissolution is performed, a solubilizer is added, a second oil phase is obtained, puerarin, a stabilizer, a penetration enhancer and a cationic material are added to a phosphate buffer solution and stirred to be completely dissolved, and a second water phase is obtained; (3) the first oil phase is added to the first water phase, stirring is performed, a first primary emulsion is obtained, the second oil phase is added to the second water phase, stirring is performed, and a second primary emulsion is obtained; (4) the first primary emulsion is dropwise added to the second primary emulsion under the stirring condition, the mixture is uniformly stirred and cooled, and a mixed primary emulsion is obtained; (5) ultrasonic treatment is performed and the preparation is obtained. The preparation has huge membrane surface area, high drug carrying capacity, good biocompatibility and higher biological adhesiveness.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an ophthalmic preparation of puerarin and scutellarin lipid nanoparticles and a preparation method thereof. Background technique [0002] Cataract is the clouding of the lens in the eye, from transparent to opaque, which prevents light from entering the eye and affects vision. The initial turbidity has little effect on vision, and then gradually aggravates, significantly affecting vision and even blindness. Cataract is the leading cause of blindness in the world. There are about 20 million people in the world who are blind due to cataract, and another 100 million cataract patients need surgery to restore vision. In most African and Asian countries, cataract accounts for at least half of the blind. Modern pharmacological studies have shown that puerarin and scutellarin are natural non-selective calcium ion channel blockers, which have strong anti-inflammatory, anti-oxidation, anti-fibros...

AI Technical Summary

Problems solved by technology

[0003] With the development of biopharmaceuticals and the continuous deepening of eye pharmacokinetics research, a large number of research results have shown that traditional ophthalmic preparations such as eye drops, eye dressings, eye washes, eye ointments, etc., have a large loss of drug dose. , poor visual acuity, low bioavailability, etc.

Especially for eye diseases that require long-term treatment, it is difficult to achieve long-term effect even if it is used repeatedly, and the use of high-concentration drugs may also cause eye irritation and systemic toxicity.

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