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Use of a product for detecting the content of acetic acid, isobutyric acid and/or valeric acid in the preparation of a product that reveals the imbalance of intestinal microecology in patients with constipation

A technology of intestinal microecology and isobutyric acid, which is applied in the fields of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of large individual differences and differentiation of intestinal microbes

Active Publication Date: 2021-03-30
湖南海路生物科技有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, due to the huge individual differences in gut microbes, it is difficult to distinguish which are caused by individual differences and which are caused by diseases from the numerous small and non-significant differences

Method used

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  • Use of a product for detecting the content of acetic acid, isobutyric acid and/or valeric acid in the preparation of a product that reveals the imbalance of intestinal microecology in patients with constipation
  • Use of a product for detecting the content of acetic acid, isobutyric acid and/or valeric acid in the preparation of a product that reveals the imbalance of intestinal microecology in patients with constipation
  • Use of a product for detecting the content of acetic acid, isobutyric acid and/or valeric acid in the preparation of a product that reveals the imbalance of intestinal microecology in patients with constipation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Introduction of intestinal microbial surgery fermentation to a specific oligo polysaccharide - Health Adult

[0041] 1, medium solution

[0042] The formulation of YCFA base medium is as follows: Cheese 10g / L, yeast extract 2.5 g / L, L-cysteine ​​hydrochloride 0.8 g / L, heme 0.05 g / l, NaCl 4.5 g / L, CaCl 2 · 6h 2 O 0.09 g / L, kh 2 PO 4 0.45 g / L, K 2 HPO 4 0.45 g / L, MGSO 4 · 7h 2 O 0.09 g / L, trace elements, solvents are deionized water, pH is natural. The final concentration of trace elements in the YCFA base medium is composed of below: 12 μg / L, cobalt amine 10 μg / L, 50 μg / L of amino benzoic acid, 50 μg / L, pyridine 150 μg / L, sulphine 50 μg / L, riboflavon 50 μg / L.

[0043] YCFA-oligosaccharide medium is an oligosaccharide or sugar alcohol added at a final concentration of 8 g / L in a YCFA base medium. Oligosaccharide refers to a single or plurality of monosaccharides linked at α-1,4 glycosidic bonds or α-1,6 glycosidism, wherein the mono...

Embodiment 2

[0110] Example 2 Intestinal microbial fermentation revealed the imbalance of intestinal microorganisms in IBS patients.

[0111] In this embodiment, the content of the metabolites in the IBS group and the feces of the health group and the fermentation fluid in the in vitro were mainly detected. It was found that although there was no difference in SCFA in the feces, the SCFA response value produced by the IBS group was significantly different from the health group of the SCFA response to a particular oligo hypoglycemic fermentation. At present, the medical community has no clinical diagnosis method for IBS, and can only determine the disease or not, and there is no clear conclusion on the relationship between IBS and intestinal flora. In the present invention, the in vitro fermentation can separate the IBS and normal groups from the normal group, and reveal the intestinal micro-alias of the IBS patient in the normal group of microorganisms. Disorder. Although the difference in the...

Embodiment 3

[0134] Example 3 The intestinal microbial fermentation revealed the intestinal microforfaction of constipation patients with a certain degree of imbalance.

[0135] In this embodiment, the content of the metabolites in the constipation group and the health group and the metabolites in the fermentation of the health group were mainly detected. It was found that although there was no difference in SCFA in the feces, the constipation group had significant differences in the SCFA response value produced by specific oligosaccharide fermentation in in vitro fermentation.

[0136] 1, preparation of feces samples

[0137] The human fecal sample is provided by 14 healthy volunteers (health groups) and constipation patients (constipation group). Volunteers ask for healthy, and there is no antibiotics, probiotics, etc. within 1 month before the sampling. Fecool sample preparation method as in Example 1.

[0138] 2. Determination of SCFA content in feces sample

[0139] The SCFA content was m...

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Abstract

The invention discloses an in-vitro simulated culture method for enteric microorganisms. The method comprises the steps of inoculating an excrement sample into a fermentation culture medium, carryingout anaerobic fermentation culture at 37 DEG C, so as to obtain fermentation liquid containing enteric microorganisms, wherein the fermentation culture medium is a YCFA basic culture medium containing6g / L-10g / L of carbohydrate, and carbohydrate is polysaccharide, oligosaccharide or sugar alcohol. By detecting a series respond values of the enteric microorganisms to a specific carbon source in anin-vitro simulated fermentation system, the metabolic capability of the enteric microorganisms and the level of probiotics are directly reflected, and direct evaluation of the functions of a complex ecosystem, namely the enteric microorganisms, is realized.

Description

Technical field [0001] The present invention relates to an intestinal microorganism in vitro simulation culture method. Background technique [0002] With the development of molecular biology techniques, especially the sequencing of human genome sequencing technology and human intestinal microorganisms, we have a more profound understanding of the factors affecting human health. The intestinal microbes are a very close factor that is a very close relationship with the health relationship between the human body itself. At present, the intestinal microorganism has affected the transformation efficiency of energy in food, affecting the body weight of the host, participating in metabolic syndrome, fatty liver, chronic colitis, intestinal cancer and so on. Even the metabolism of pentinhydroxyamine is involved in the metabolism of pentinol and affects the mental state of the host. Therefore, the concept of human health has a new meaning, except for its own organs, it should also contai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 朱立颖王欣刘伟皮雄娥费迪波陈镜刚李降龙
Owner 湖南海路生物科技有限公司
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