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Pinolenic acid lutein ester enriched pine-seed oil and preparation method thereof

A technology of lutein lutein and pine nut oil, which is applied in the field of pine nut oil rich in lutein lutein pinovenate and its preparation field, can solve the problems of low stability and low activity of lutein, and achieve Simple operation, low reaction temperature, and the effect of overcoming low stability

Inactive Publication Date: 2018-06-05
吉林派诺生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The present invention provides a kind of pine nut oil rich in pinolenic acid lutein ester and its microencapsulation method, in order to solve the problem of low stability and low activity of lutein, endow pine nut oil with the active ingredient of lutein, Generated a new active ingredient—pinotenic acid lutein ester monomer, which was microencapsulated without adding emulsifiers

Method used

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  • Pinolenic acid lutein ester enriched pine-seed oil and preparation method thereof

Examples

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Effect test

Embodiment 1

[0029] (1) Esterification and deacidification of lutein: first, add 3.51KG lutein and 1000L absolute ethanol into the esterification kettle, heat to 30°C, keep stirring for 0.5 hours, cool down to 60°C to obtain a mixture, Next, add 1000KG pine nut crude oil with an acid value of 30KOH mg / g into the mixture of the esterification tank, and at the same time add 351g of immobilized lipase Lipozyme RMIM, keep stirring at 30°C for 4 hours, stop heating, cool down to normal temperature, and the reaction is complete. get the mixture;

[0030] (2) Separation of the mixture: Stir and add 1000 L of saturated saline at a temperature of 65°C to the esterification kettle, let the layers stand for 2 hours, recover the solvent from the lower layer, repeat the same method for washing once, discard the lower layer, turn on the vacuum pump, and heat up to 65°C Dehydrate at ℃ for 2 hours, until the water content is ≤0.1% by sampling, put the dehydrated material into the freezer, turn on the free...

Embodiment 2

[0032] (1) Esterification and deacidification of lutein: First, add 2.1KG lutein and 100L n-hexane into the esterification kettle, heat to 57.5°C, stir and keep the temperature for 1.7 hours, cool down to 60°C to obtain the mixture, and then 1000KG pine nut crude oil with an acid value of 60KOH mg / g was added to the mixture of the esterification kettle, and 105g of immobilized lipase Lipozyme RMIM was added at the same time, kept at 45°C and stirred for 14 hours under normal pressure to stop heating, and anhydrous sodium sulfate was added during the process. After the reaction is complete, the mixture is obtained;

[0033] (2) Separation of the mixture: turn on the vacuum pump, heat up to 75°C and distill for 3 hours to recover n-hexane, put the material in the esterification kettle into the freezer, turn on the freezer and stir to cool the material down to 10°C, start centrifugal filtration, discard the solid, and the filtrate That is, pine nut oil rich in pinolenic acid lute...

Embodiment 3

[0035] (1) Esterification and deacidification of lutein: First, add 2.92KG lutein and 500L acetone into an esterification kettle, heat to 85°C, keep stirring for 3 hours, then cool down to 60°C to obtain a mixture for later use. Next, add 1000KG pine nut crude oil with an acid value of 50KOH mg / g to the mixture of the esterification kettle, and at the same time add 39g of immobilized lipase Lipozyme RMIM, keep stirring at 60°C for 24 hours and stop heating, and add anhydrous sodium sulfate during the reaction , the reaction is completed to obtain the mixture;

[0036] (2) Separation of the mixture: turn on the vacuum pump, heat up to 85°C and distill for 4 hours to recover acetone, put the material in the esterification kettle into the freezer, turn on the freezer to stir the material to cool down to 0°C, start centrifugal filtration, discard the solid, and the filtrate is ready Pine nut oil rich in pinolenic acid lutein ester.

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Abstract

The invention relates to pinolenic acid lutein ester enriched pine-seed oil and a preparation method thereof, and belongs to the fields of grease processing and food chemistry. The preparation methodcomprises the following steps of firstly, adding lutein and a solvent into an esterification kettle, heating, agitating, maintaining a temperature for 0.5 to 3 hours, and lowering the temperature to be smaller than and equal to 60 DEG C, so as to obtain a mixed material; secondly, adding crude pine-seed oil into the mixed material, meanwhile, adding fixed and specific lipase, maintaining a normal-temperature agitation reaction at 30 to 60 DEG C, stopping heating, lowering to normal temperature, so as to obtain a mixture after the reaction is completed, removing the solvent from the mixture through water scrubbing, layering and drying steps after the temperature is lowered, and removing the lipase and unreacted lutein through freezing filtration, so as to obtain the pinolenic acid lutein ester enriched pine-seed oil. The preparation method has the beneficial effects that the acid value of the pine-seed oil is decreased by esterification acid removal; the refining loss of grease is reduced; the pinolenic acid lutein ester is formed; an effective component of the pinolenic acid is given to the lutein; the reaction temperature is low; the high unsaturated fatty acid and the lutein cannot be destroyed; the operation is simple, and the industrialized production is easy.

Description

technical field [0001] The invention belongs to the fields of oil processing and food chemistry, and relates to a pine nut oil rich in pinolanic acid lutein ester and a preparation method thereof, in particular to the esterification of free fatty acids and lutein in pine nut oil catalyzed by immobilized enzymes The invention discloses a method for preparing pinolenic acid lutein ester functional oil by reaction. Background technique [0002] Pinolenic acid is an all-cis 5, 9, 12 octadecatrienoic acid, which is different from unsaturated fatty acids in all plants on the earth. It only exists in pine nut oil, and it can not only lower cholesterol ( TC), triglycerides (TG), increase high-density lipoprotein (HDL), but also inhibit and eliminate the adverse effects of other unsaturated fatty acids on the body. At the same time, the latest research shows that it also has anti-inflammatory, antipyretic, analgesic, anti-fungal and viral infections, and promotes the excretion funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11B1/10C11B3/00
CPCC11B1/10C11B3/001C11B3/003C11B3/008C12P7/6436
Inventor 王小锋赵尔哲
Owner 吉林派诺生物技术股份有限公司
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