Extraction and separation method of tea polyphenol

A separation method and technology for tea polyphenols, applied in the direction of organic chemistry, etc., can solve the problems of limited application of tea polyphenols, low bioavailability, poor stability, etc., and achieve the effects of great social and economic value, uniform particle size and good gloss.

Pending Publication Date: 2020-11-20
SICHUAN UNIV
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Problems solved by technology

Although tea polyphenols have strong antioxidant properties and a variety of biological health benefits, their poor oil solubility, poor stability in aqueous solution, and low bioavailability limit the application of tea polyphenols.

Method used

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  • Extraction and separation method of tea polyphenol
  • Extraction and separation method of tea polyphenol
  • Extraction and separation method of tea polyphenol

Examples

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Embodiment 1- Embodiment 6

[0024]In the following examples, tea polyphenols, formaldehyde, and amino acids are selected as reaction raw materials, and the particle size control of tea polyphenol micro-nano particles is realized by adjusting the concentration of amino acids. The specific implementation method is as follows: Take 40.0 mL (2.0 mg / mL) of the tea polyphenol solution after adjusting the concentration and add it to a 100 mL round bottom flask, add 30 μL formaldehyde (37%) dropwise, control the reaction temperature at 25°C, and stir for 5-10 After 1 min, 1.0 mL of amino acid solution was added. After the amino acid is added, as the micro-nano particles of tea polyphenols are formed in the aqueous solution, the reaction solution quickly turns from clear and transparent to a milk-like milky white solution. After continuing to stir the solution for 0.5-1 h, the tea polyphenol micro-nanoparticles were collected by centrifugation (8000-10000 rcf×g, 20 min). The tea polyphenol micro-nanoparticles ob...

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Abstract

The invention discloses an extraction and separation method of tea polyphenol. The method comprises the following steps: an aldehyde compound and an amino compound are added into a tea polyphenol leaching solution to form tea polyphenol polymer particles; the particles can be quickly separated by means of filtration, centrifugation and the like; and the obtained tea polyphenol particles can be purified for multiple times through a dissolving-reassembling process. The method can also be combined with various existing tea polyphenol extraction, separation and purification methods, and tea polyphenol particles are economically and efficiently extracted and separated; the obtained particles have multiple functional activities of tea polyphenol molecules, and can be widely applied to the fieldsof foods, cosmetics and medicines; the method is greatly different from a traditional tea polyphenol extraction scheme in thought and technical method, the traditional technical scheme is greatly simplified, energy and solvent consumption is effectively reduced, the period is shortened, and the production cost is reduced; and meanwhile, reform and innovation of a traditional extraction process technology can be inspired.

Description

technical field [0001] The invention relates to the extraction technology of plant active components, which is a new tea polyphenol extraction and separation method which combines the polymerization-induced self-assembly technology and the traditional tea polyphenol extraction technology. The invention also relates to the technical field of functionalization and nanometerization of tea polyphenols. Background technique [0002] Tea polyphenols are plant polyphenols extracted from tea leaves, which have microbial inhibition, antioxidant, anti-inflammatory and anti-tumor activities, and are one of the research hotspots in the fields of food science and biomedicine. Tea polyphenols are licensed antioxidant additives widely used in food processing and cosmetics manufacturing. The traditional tea polyphenol extraction process mainly includes tea polyphenol extraction, extraction, adsorption and desorption, concentration and drying process. With the development of society, the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 李旭东陈光灿易增陈翔宇
Owner SICHUAN UNIV
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