Rapid propagation method of rhizomes of Enshi polygonatum sibiricum

Abstract

The invention relates to a rapid propagation method of rhizomes of Enshi polygonatum sibiricum. Aggravation of disease and insect damage and germplasm degradation easily occur in conventional rhizomepropagation, a rapid propagation system is established with rhizome terminal buds and stem tips used as explants in tissue culture, more available polygonatum sibiricum terminal bud scales are selected as explants test materials in consideration of the current situations of single explants source, large test material consumption and low propagation efficiency, adventitious roots are obtained by inducing bud scale differentiation, the adventitious roots are further induced to produce different types of differentiated bodies such as rhizomes with adventitious roots and terminal buds, adventitious bud clusters and calluses in multiple forms. Those types of differentiated bodies are continuously cultured respectively in Enshi and can continuously produce new adventitious bud clusters, rhizomeswith terminal buds and multiple types of calluses, so that a high-frequency regeneration system of excised roots, stems and leaves of polygonatum sibiricum is established. Roots, stems, leaves, calluses and rhizomes which are obtained with the method can be converted to grow in different culture periods.

Description

technical field [0001] The invention relates to a method for rapidly multiplying Enshi Polygonatum stems and tubers. Background technique [0002] Polygonatum sibiricum (scientific name: Polygonatum sibiricum), has another name: chicken head Polygonatum, yellow chicken dish, pen tube dish, claw ginseng, tiger ginger, chicken claw ginseng. It is a plant of the genus Polygonatum, the rhizome is horizontal, cylindrical, and the nodules are enlarged. Leaves whorl, sessile. Medicinal plants have the functions of invigorating the spleen, moistening the lungs and promoting body fluid. Polygonatum is used as medicine with rhizomes. It has the functions of invigorating qi and nourishing yin, invigorating the spleen, moistening the lungs and benefiting the kidney. It is used to treat weak spleen and stomach, tiredness and fatigue, dry mouth and lack of food, dry cough due to lung deficiency, insufficient essence and blood, internal heat and thirst. Very effective for diabetes. P...

AI Technical Summary

Problems solved by technology

Because the source of Rhizoma Polygonatum on the market is still mainly based on mining wild resources, this has led to excessive predatory mining of wild resources, causing the wild resources of Rhizoma Polygonatum to be on the verge of extinction. For example, it is difficult to directly propagate Polygonatum with seeds and it is prone to species degeneration; the rhizome propagation method generally adopted in production also has the problems that root diseases and insect pests increase year by year after continuous planting, and the quality declines, which in turn affects the quality of Polygonatum products. and output

In addition, the wild growth environment of sealwort has been severely damaged, and it is difficult to restore the natural population growth, which further affects the sustainable utilization and in-depth development of sealwort resources.