Preparation jointly using glycine and amoxicillin

A technology for amoxicillin and amoxicillin sensitivity, applied in the direction of medical preparations containing active ingredients, resistance to vector-borne diseases, organic active ingredients, etc.

Inactive Publication Date: 2018-06-08
厦门显德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no reports of glycine-promoting antibiotics inhibiting the growth ...

Method used

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  • Preparation jointly using glycine and amoxicillin
  • Preparation jointly using glycine and amoxicillin
  • Preparation jointly using glycine and amoxicillin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Determination of drug resistance of Edwardsiella tarda EIB202

[0026] Edwardsiella tarda is a Gram-negative short bacillus. It was first reported by Hoshina (1 96 2a), and it is related to reddisease of Japanese eel. From the first report to now, the fungus has caused diseases in more than 20 kinds of fish, such as eel, flounder, tilapia, Chinese soft-shelled turtle, carp, etc., causing huge losses to aquaculture. Edwardsiella tarda is also a common pathogenic bacteria in humans and fish, which directly poses a threat to human health.

[0027] Firstly, the minimum inhibitory concentration of Edwardsiella tarda to various antibiotics was determined. The results showed that the minimum inhibitory concentration of Edwardsiella tarda EIB202 to ampicillin was 3.12 μg / mL, the minimum inhibitory concentration to kanamycin was 12.5 μg / mL, and the minimum inhibitory concentration to tetracycline was 125 μg / mL. The minimum inhibitory concentration of chloramphenicol was 50μg / ...

Embodiment 2

[0029] Glycine and / or glucose increase susceptibility to amoxicillin in Edwardsiella tarda

[0030] Preparation of test samples: Pick a single colony of Edwardsiella tarda EIB202 from the LB plate and inoculate it in 5mL of LB medium, shake and culture at 200rpm at 30°C for 24h to reach saturation. Collect the bacterial liquid by centrifugation, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD value of the bacterial solution to 0.2, and then pack them separately 5mL in a test tube for later use.

[0031] The prepared samples were divided into 5 groups, of which 2 groups were the control group, which were without adding any substances and adding amoxicillin; the other 3 groups were the experimental group, in the case of adding amoxicillin, adding glycine, glucose, Glycine and Glucose. After incubating in a shaker at 30°C and 2...

Embodiment 3

[0033] Glycine and / or glucose increase susceptibility to amoxicillin in Escherichia coli and its Escherichia coli clinical strains

[0034] (1) Glycine and / or glucose can increase the sensitivity of Escherichia coli to amoxicillin

[0035] Preparation of test samples: Pick a single colony of Escherichia coli from the LB plate and inoculate it in 5mL of LB medium, shake and culture at 200rpm at 37°C for 16h to reach saturation. Collect the bacterial liquid by centrifugation, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD value of the bacterial solution to 0.2, and then pack them separately 5mL in a test tube for later use.

[0036] The prepared samples were divided into 5 groups, of which 2 groups were the control group, which were without adding any substances and adding amoxicillin; the other 3 groups were the experimental ...

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Abstract

The invention provides a preparation jointly using glycine and amoxicillin, and relates to a micromolecule substance, such as glycine. The glycine can be used for improving the sensitivity of bacteriato penicillin antibiotics, such as amoxicillin; the glycine and glucose can be used for improving the sensitivity of bacteria to penicillin antibiotics, such as amoxicillin. The preparation jointly using the glycine and the amoxicillin is prepared from the glycine and/or the glucose, and the penicillin antibiotics, such as amoxicillin. The preparation has the advantages that by adding the glycineand/or the glucose, the survival rate of edwardsiella tarda is obviously reduced when the edwardsiella tarda is treated by the amoxicillin, and the glycine and/or the glucose can improve the sensitivity of the edwardsiella tarda to the amoxicillin; after the glycine and/or the glucose is added, the survival rate of escherichia coli is obviously reduced when the escherichia coli is treated by theamoxicillin,, and the glycine and/or the glucose can improve the sensitivity of the escherichia coli to the amoxicillin.

Description

technical field [0001] The invention relates to a small molecular substance glycine, in particular to a preparation of glycine combined with amoxicillin which can improve the susceptibility of bacteria to antibiotics. Background technique [0002] Although the use of antibiotics plays an essential role in the protection of human health and life and intensive animal farming, due to the abuse and misuse of antibiotics, it has become a threat to human health, livestock and poultry farming, aquaculture and the ecological environment. The key factor. Therefore, the control of bacterial antibiotic resistance is very important. [0003] At present, antibiotics are widely used in animal husbandry. On the one hand, some antibiotics are essential as veterinary drugs to control bacterial infections; on the other hand, some antibiotics can promote animal growth as feed drug additives. The extensive use of antibiotics has caused a large number of sensitive bacteria to die, and drug-re...

Claims

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Application Information

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IPC IPC(8): A61K31/43A61K31/198A61K31/7004A61P31/04
CPCA61K31/198A61K31/43A61K31/7004A61K2300/00Y02A50/30
Inventor 谢荔朋
Owner 厦门显德生物科技有限公司
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