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Method for immobilising protein of particle

一种蛋白质固定、蛋白质的技术，应用在化学仪器和方法、应用能量的化学/物理/物理化学过程、仪器等方向，能够解决处理时间长等问题，达到防止聚集的效果

Inactive Publication Date: 2018-06-08
AMOLIFESCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the protein immobilization methods of Thermo and Life Technology have problems in that protein-protein aggregates or protein-particle aggregates may be formed due to non-specific binding, and the method of Life Technology has the disadvantage of long processing time

Method used

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  • Method for immobilising protein of particle
  • Method for immobilising protein of particle
  • Method for immobilising protein of particle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of protein-immobilized particles

[0039] 1 ml of 20 mg / ml magnetic particles (AMO-Mag, purchased from AMOGREENTECH) were washed three times with 1˜5 ml of PBS (potassium buffered saline). After that, add 225 μl of PBS and 225 μl of mixed solution (pH4.7, 0.1M MES+0.9%NaCl+0.02%T-20; add surfactant T-20 to prevent the electrostatic combination by protein and protein and magnetic Aggregation due to nonspecific binding between particles), and then sufficiently sonicated, and 1 mg or 2 mg of IgG (immunoglobulin G), which is an anti-RBC (red blood cell) antibody (manufactured by Fitzgerald), was added thereto, and then sonicated again deal with.

[0040] Next, 50 μl of 10 mg / ml EDC (1-ethyl-3-(3-dimethylamino Propyl) carbodiimide hydrochloride), followed by mixing at room temperature for 30 minutes (sonication was performed at 5-minute intervals after the initial sonication). Subsequently, protein-immobilized particles were prepared by washing thre...

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Abstract

The present invention relates to a method for immobilising protein of a particle, and more specifically to a method for immobilising antibody onto a magnetic particle. The method for immobilising protein of a particle of the present invention is characterised by a feature of protein not sticking together due to non-specific protein-protein and protein-particle binding so that a relatively small amount of protein can be immobilised onto a particle.

Description

technical field [0001] The present invention relates to a method for immobilizing proteins on particles, more particularly to a method for immobilizing antibodies on magnetic particles. Background technique [0002] Protein-immobilized particles for immunochromatographic analysis are used in the medical care field to identify diseases or detect changes in diseases, and are also used for simple analysis in various fields including food and bioprocessing fields, environmental fields, etc. small amount of analyte. Specifically, in the field of healthcare, its applications have expanded to pregnancy, ovulation, infectious diseases, drug abuse, acute myocardial infarction, cancer, and more. For example, the particles described above can be used to isolate disease markers in blood. [0003] Among these particles, magnetic particles have attracted much attention as labeling materials for binding assays due to the advantages of easy control, high biocompatibility, and high sensiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/558B01J19/10B01J31/12
CPCG01N33/54353B01J31/0247B01J2231/34B01J31/12G01N33/531G01N33/553
Inventor 金成一文鲜
Owner AMOLIFESCI CO LTD