Preparation method of hemoglobin polypeptide powder

A technology of hemoglobin and polypeptide powder, which is applied in the field of preparation of hemoglobin polypeptide powder, can solve problems such as environmental pollution and loss of valuable nutritional resources, and achieve the effects of high purity, enhanced immunity, and small molecular weight

Inactive Publication Date: 2018-06-12
浦江县美泽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, animal blood, especially sheep blood, is mostly discharged or discarded in the form of sewage, resulting in the loss of a large amount of valuable nutritional resources and causing serious pollution to the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: the preparation method of hemoglobin polypeptide powder, its preparation method comprises the following steps:

[0011] 1) Preparation of hemoglobin: Add trisodium citrate to sheep blood, wherein the mass ratio of trisodium citrate to sheep blood is 3 / 100, stir, then centrifuge at a relative centrifugal force of 4000-6000g for 6-10min, take the lower layer of liquid to obtain hemoglobin;

[0012] 2) Preparation of enzymolysis solution: add 35 parts of water to 2 parts of hemoglobin, adjust the pH to 7, heat to 90°C for 30 minutes, then cool down to 55°C, add thiol protease accounting for 3% of the mass of hemoglobin to react for 2 hours, and then Add papain, which accounts for 2% of the hemoglobin mass, and hydrolyze it for 7 hours at 45°C and pH 8. After the reaction, the enzyme activity is inactivated, and the supernatant is filtered to obtain an enzymolysis solution. Under this condition, thiol protease and papaya Protease Two kinds of proteases are en...

Embodiment 2

[0014] Embodiment 2: the preparation method of hemoglobin polypeptide powder, its preparation method comprises the following steps:

[0015] 1) Preparation of hemoglobin: Add trisodium citrate to sheep blood, wherein the mass ratio of trisodium citrate to sheep blood is 5 / 100, stir, then centrifuge at a relative centrifugal force of 4000-6000g for 6-10min, take the lower layer of liquid to obtain hemoglobin;

[0016] 2) Preparation of enzymolysis solution: add 45 parts of water to 5 parts of hemoglobin, adjust pH to 9, heat to 95°C and keep for 40min, then cool down to 60°C, add thiol protease accounting for 5% of the mass of hemoglobin to react for 6h, and then Add papain, which accounts for 5% of the hemoglobin mass, and hydrolyze it for 10 hours at 55°C and pH 8. After the reaction, the enzyme activity is inactivated, and the supernatant is filtered to obtain an enzymatic solution. Under this condition, sulfhydryl protease and papaya Protease Two kinds of proteases are enz...

Embodiment 3

[0018] Embodiment 3: the preparation method of hemoglobin polypeptide powder, its preparation method comprises the following steps:

[0019] 1) Preparation of hemoglobin: Add trisodium citrate to sheep blood, wherein the mass ratio of trisodium citrate to sheep blood is 4 / 100, stir, then centrifuge at a relative centrifugal force of 4000-6000g for 6-10min, take the lower layer of liquid to obtain hemoglobin;

[0020] 2) Preparation of enzymolysis solution: add 37 parts of water to 3 parts of hemoglobin, adjust pH to 8, heat to 93°C for 34min, then cool down to 57°C, add thiol protease accounting for 5% of hemoglobin mass, and then add 5% of hemoglobin 1‰ pullulan polysaccharide and 2,4-hexadienoic acid with hemoglobin quality 0.3‰, react for 3 hours, then add papain, which accounts for 2% of hemoglobin, and hydrolyze for 8 hours at 48°C and pH 8, after the reaction Inactivate the enzyme activity, filter the supernatant to obtain the enzymolysis solution, the added 2,4-hexadie...

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PUM

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Abstract

The invention discloses a preparation method of hemoglobin polypeptide powder, comprising: preparing hemoglobin, preparing an enzymatic hydrolysis solution and preparing polypeptide powder. The preparation method has the advantages that the preparation method disclosed herein is simple, and the prepared polypeptide has low molecular weight and high purity, can be easily absorbed by human bodies, is suitable for direct use and has significant economic benefit.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a preparation method of hemoglobin polypeptide powder. Background technique [0002] Blood protein has a very high biological value, contains 8 kinds of essential amino acids needed by the human body, and its composition is balanced, which is a high-quality protein source. However, at present, animal blood, especially sheep blood, is mostly discharged or discarded in the form of sewage, resulting in the loss of a large amount of valuable nutritional resources and causing serious pollution to the environment. In sheep blood, hemoglobin accounts for 60-70% of its total protein content. Hemoglobin bioactive peptides have many extremely important functional activities, such as opioid activity, ACE inhibitory activity, bradykinin-enhancing activity, bacterial growth-stimulating activity, analgesic activity, antibacterial activity, etc. Processing hemoglobin not only contributes to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/805C07K1/18
CPCC07K14/805C12P21/06
Inventor 谢雨欣
Owner 浦江县美泽生物科技有限公司
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