Tissue culture propagation expanding method for dendrobium crepidatum

A Dendrobium rosea tissue culture technology, applied in horticultural methods, botany equipment and methods, seed immunity, etc., can solve the problems of Dendrobium rosea resource reduction, difficult germination and growth, long growth cycle, etc., to increase speed and breeding quantity, The effect of pure varieties and saving raw materials

Inactive Publication Date: 2018-06-15
康美药业(昆明)种质资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, Dendrobium rosea in the domestic market mainly relies on wild excavation, and a few are artificially cultivated. Generally, they are propagated by division propagation and cuttings. There are few seedlings for propagation, low reproduction rate, and ...

Method used

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  • Tissue culture propagation expanding method for dendrobium crepidatum
  • Tissue culture propagation expanding method for dendrobium crepidatum
  • Tissue culture propagation expanding method for dendrobium crepidatum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A method for the proliferation of dendrobium rose tissue culture, comprising:

[0052] Explant selection steps: select robust female plants of Dendrobium roseus 2-4 years old, artificially pollinate Dendrobium rosebush during the flowering period in mid-June, let it grow fruit pods, and cultivate for 3-4 months. After the fruit pods are ripe, they are picked and stored at 5°C for 2 weeks;

[0053] Explant disinfection steps: take out the fruit pods and put them in a culture bottle, first soak the fruit pods with detergent water for 18 minutes, brush the surface of the seed shell with a brush, and rinse with tap water for 15 minutes, then wash the fruit pods with a concentration of 75% for medical use Soak in alcohol for 50s, rinse with sterile water for 3 times, then use 2% sodium hypochlorite to disinfect the pod shell once for 1 min, then rinse with sterile water for 3 times, and then use 0.2% mercuric chloride for the second time Disinfect for 6 minutes, then rinse ...

Embodiment 2

[0059] A method for the proliferation of dendrobium rose tissue culture, comprising:

[0060] Explant selection steps: select robust female plants of Dendrobium roseus 2-4 years old, artificially pollinate Dendrobium rosebush during the flowering period in mid-June, let it grow fruit pods, and cultivate for 3-4 months. After the fruit pods are ripe, they are picked and stored at 3°C ​​for 2 weeks;

[0061] Explant disinfection steps: take out the fruit pods and put them in a culture bottle, first soak the fruit pods with detergent water for 15 minutes, brush the surface of the seed shell with a brush, and rinse with tap water for 10 minutes, then wash the fruit pods with a concentration of 75% for medical use Soak in alcohol for 33s, rinse with sterile water for 3 times, then use 1% sodium hypochlorite to disinfect the pod shell once for 2 minutes, then rinse with sterile water for 3 times, and then use 0.1% mercuric chloride for a second time Disinfect for 8 minutes, then ri...

Embodiment 3

[0067] A method for the proliferation of dendrobium rose tissue culture, comprising:

[0068] Explant selection steps: select robust female plants of Dendrobium roseus 2-4 years old, artificially pollinate Dendrobium rosebush during the flowering period in mid-June, let it grow fruit pods, and cultivate for 3-4 months. After the fruit pods are ripe, they are picked and stored at 7°C for 3 weeks;

[0069] Explant disinfection steps: take out the fruit pods and put them in a culture bottle, first soak the fruit pods in detergent water for 20 minutes, brush the surface of the seed shell with a brush, and rinse with tap water for 15 minutes, then wash the fruit pods with a concentration of 75% for medical use Soak in alcohol for 60s, rinse with sterile water for 4 times, then use 3% sodium hypochlorite to disinfect the fruit pod shell once for 1 min, then rinse with sterile water for 4 times, and then use 0.3% mercuric chloride for a second time Disinfect for 6 minutes, then rins...

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Abstract

The invention discloses a tissue culture propagation expanding method for dendrobium crepidatum. The method comprises the steps of an explant selection step, an explant sterilization step, a sowing step, a secondary culture step, a rooting culture step, and a seedling hardening domestication and transplanting step. According to the method provided by the invention, the breeding speed and breedingquantity of seedlings of the dendrobium crepidatum can be effectively improved, the stability of germplasm is controlled from a female parent source, the quality of the seedlings can be guaranteed, industrialized production of the high-quality seeds and seedlings of the dendrobium crepidatum can be realized, requirements for production can be met, and a current situation of resource scarcity can be relieved.

Description

technical field [0001] The invention relates to the field of tissue culture and seedling cultivation, in particular to a method for tissue culture and propagation of Dendrobium rosea. Background technique [0002] Dendrobium crepidatum Lindl.ex Paxt. is an orchid, mainly distributed in China, India, Nepal, Bhutan, Myanmar, Thailand, Laos, and Vietnam. The stem is pendulous, fleshy and thick, turquoise, cylindrical, unbranched, with many nodes; the raceme is very short, from the old stem with fallen leaves, with 1-4 flowers, the petals are thick, spreading, Sepals and petals white, lavender in the upper part, lavender above the middle part of the lip, golden yellow below the middle part, flowering period March-April. [0003] At present, Dendrobium rosea in the domestic market mainly relies on wild excavation, and a few are artificially cultivated. Generally, they are propagated by division propagation and cuttings. There are few seedlings for propagation, low reproduction r...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/08
Inventor 许冬瑾严新雷连海杜永魏
Owner 康美药业(昆明)种质资源有限公司
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