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Chitosan/silk fibroin stent with loaded curcumin/gelatin microsphere compound, and preparation method and applications thereof

A technology of silk fibroin and curcumin, applied in the field of skin care products, can solve the problems of curcumin application limitation, low water solubility and low solubility of curcumin, and achieve the advantages of reducing harm to human body, wide antibacterial range and simple preparation process. Effect

Inactive Publication Date: 2018-06-15
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, curcumin itself has low water solubility and is unstable under alkaline conditions. It is easily decomposed by heat and light. It is placed in a physiological fluid environment, such as low solubility in PBS solution, which greatly reduces its drug resistance. effect
The above defects have greatly restricted the clinical application of curcumin

Method used

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  • Chitosan/silk fibroin stent with loaded curcumin/gelatin microsphere compound, and preparation method and applications thereof
  • Chitosan/silk fibroin stent with loaded curcumin/gelatin microsphere compound, and preparation method and applications thereof
  • Chitosan/silk fibroin stent with loaded curcumin/gelatin microsphere compound, and preparation method and applications thereof

Examples

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Effect test

Embodiment 1

[0041] A kind of embodiment of the preparation method of the chitosan / silk fibroin scaffold of the load curcumin / gelatin microsphere complex of the present invention, the chitosan / silk fibroin of load curcumin / gelatin microsphere complex described in this embodiment The preparation method of protein scaffold comprises the following steps:

[0042] (1) 0.2wt% NaCO at 100° C. 3 Boil in 100mL aqueous solution for 2 hours, wash silk fibroin with deionized water until neutral after degumming, and dry in an oven at 60°C to obtain silk fibroin; take 2.5g silk fibroin and add 25mL, 9.8mol / L lithium bromide aqueous solution In the process, after heating in a water bath at 60°C for 5 hours, dialyze with deionized water (the molecular weight cut-off of the dialysis bag is 8000, the dialysis time is 3 days, and change the water once a day), and then centrifuge at 25°C and 10000r / min for 10min to obtain 3.5wt% silk protein solution;

[0043] (2) Dissolve 5g of chitosan and 13g of potassi...

Embodiment 2

[0051] A kind of embodiment of the preparation method of the chitosan / silk fibroin scaffold of the load curcumin / gelatin microsphere complex of the present invention, the chitosan / silk fibroin of load curcumin / gelatin microsphere complex described in this embodiment The preparation method of protein scaffold comprises the following steps:

[0052] (1) 0.2wt% NaCO at 100° C. 3 Boil in 100mL aqueous solution for 2h, after degumming, wash silk fibroin with deionized water until neutral, dry in an oven at 60°C to obtain silk fibroin; take 1g of silk fibroin and add it to 25mL, 9.8mol / L lithium bromide aqueous solution, After heating in a water bath at 60°C for 3 hours, dialyze with deionized water (the molecular weight cut-off of the dialysis bag is 10,000, the dialysis time is 3 days, and change the water once a day), and then centrifuge at 25°C and 10,000r / min for 10min to obtain a 2wt% silk fibroin solution ;

[0053] (2) Dissolve 5g of chitosan and 13g of potassium iodide (K...

Embodiment 3

[0059] A kind of embodiment of the preparation method of the chitosan / silk fibroin scaffold of the load curcumin / gelatin microsphere complex of the present invention, the chitosan / silk fibroin of load curcumin / gelatin microsphere complex described in this embodiment The preparation method of protein scaffold comprises the following steps:

[0060] (1) 0.2wt% NaCO at 100° C. 3 Boil in 100mL aqueous solution for 2h, after degumming, wash silk fibroin with deionized water until neutral, dry in an oven at 60°C to obtain silk fibroin; take 7.5g silk fibroin and add 25mL, 9.8mol / L lithium bromide aqueous solution After heating in a water bath at 60°C for 4 hours, dialyze with deionized water (the molecular weight cut-off of the dialysis bag is 12,000, the dialysis time is 3 days, and change the water once a day), then centrifuge at 25°C and 10,000r / min for 10min to obtain 10wt% silk fibroin protein solution;

[0061] (2) Dissolve 5g of chitosan and 13g of potassium iodide (KI) in ...

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Abstract

The invention discloses a chitosan / silk fibroin stent with a loaded curcumin / gelatin microsphere compound, and a preparation method and applications thereof. The preparation method comprises followingsteps: mixing a silk fibroin solution with a chitosan solution according to a volume ratio of 1:1 to obtain a chitosan / silk fibroin composite solution; dropwise adding a curcumin solution with a concentration of 10-50 mg / mL into gelatin microspheres, placing the system in an environment with a temperature of 4-25 DEG C overnight, then freeze-drying the solution to obtain a curcumin / gelatin microsphere compound; then adding the curcumin / gelatin microsphere compound into the chitosan / silk fibroin composite solution, evenly stirring, and then freeze-drying the solution to obtain the chitosan / silk fibroin stent with a loaded curcumin / gelatin microsphere compound. The porosity of the stent is 75 to 95%; the aperture is 50 to 200 [mu]m, and moreover, the stent has a long-term antibacterial function and a drug sustained-release function, and can be used to prepare skin wound dressing.

Description

technical field [0001] The invention relates to the field of skin care products, in particular to a chitosan / silk fibroin scaffold loaded with curcumin / gelatin microsphere complex, a preparation method and application thereof. Background technique [0002] The skin is the largest organ of the human body, mainly divided into the epidermis, dermis and subcutaneous tissue, and has important barrier, mechanical, sensory and regulatory functions for the human body. But the skin is one of the most fragile and vulnerable organs of the human body. Skin ulceration, wounds, surgical injuries and burns are important causes of skin damage, and burns are the main cause of skin damage. According to statistics, the number of people who die from burns in the world is about 300,000 every year, while there are about 10 million burn patients in my country every year, of which 3.2 million need skin transplantation. Infection is an important factor restricting the healing of burn skin, because ...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0023A61L26/0047A61L26/0066A61L2300/216A61L2300/404A61L2300/602C08L5/08C08L89/00
Inventor 郭瑞李丹蓝咏毛宇马春茗
Owner BEOGENE BIOTECH GUANGZHOU
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