Antibacterial and hemostatic bifunctional material and preparation method thereof
A dual-functional material and cellulose-based technology, which is applied in pharmaceutical formulations, medical science, absorbent pads, etc., can solve the problems of antibacterial hemostasis, coagulation dysfunction, secondary trauma, etc., and achieve good biocompatibility and Non-toxic, easy to scale up industrial production, long-lasting effect of antimicrobial properties
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Embodiment 1
[0024] (1) Mix 0.25g of sodium periodate, 0.25g of polyethyleneimine and 2g of bacterial cellulose in a gel state into 20mL of deionized water, and react in an oven at 80°C for 50 minutes, and the obtained modified bacterial cellulose is coagulated. In colloidal state, wash with deionized water three times, and oven-dry at 60°C to obtain a dual-function bacterial cellulose gel with antibacterial and hemostatic properties.
[0025] (2) Antibacterial test procedure: add 33 g of agar into the container, add deionized water to 1 L, and stir evenly to prepare a solid medium. Add 10 g of tryptone, 5 g of beef extract, and 5 g of sodium chloride into the container, then add deionized water to 1 L, and stir evenly to prepare a liquid medium. The liquid medium and solid medium were divided into 18cm test tubes and Erlenmeyer flasks, and sterilized at 121°C for 30min after sealing. Take out the slant of the strain stored at 4°C, perform aseptic operation, and inoculate it on 3 culture ...
Embodiment 2
[0032] (1) Mix 0.25g of sodium periodate, 0.25g of polyethyleneimine, and 2g of powdered absorbent cotton cellulose into 20mL of deionized water, and react in an oven at 90°C for 20 minutes, and the obtained modified absorbent cotton cellulose is in a powder state , washed three times with deionized water, and oven-dried at 60°C to obtain bifunctional cellulose with antibacterial and hemostatic properties.
[0033] (2) Antibacterial test procedure: add 33 g of agar into the container, add deionized water to 1 L, and stir evenly to prepare a solid medium. Add 10 g of tryptone, 5 g of beef extract, and 5 g of sodium chloride into the container, then add deionized water to 1 L, and stir evenly to prepare a liquid medium. The liquid medium and solid medium were divided into 18cm test tubes and Erlenmeyer flasks, and sterilized at 121°C for 30min after sealing. Take out the slant of the strain stored at 4°C, perform aseptic operation, and inoculate it on 3 culture medium slants. ...
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