Method for extracting tea polyphenol in tea residues by using IL (ionic liquid) assisted ATPS (aqueous two phase system)

An ionic liquid, two-phase system technology, applied in the direction of tea extraction, tea, application, etc., can solve the problems of reducing the quality of tea polyphenols, difficult to popularize and apply, and high extraction temperature, and achieves improved binding force, high extraction efficiency, high extraction temperature. The effect of purity

Inactive Publication Date: 2018-06-19
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, organic solvent extraction needs to consume a large amount of organic solvent, and the extraction temperature is too high, which of...

Method used

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  • Method for extracting tea polyphenol in tea residues by using IL (ionic liquid) assisted ATPS (aqueous two phase system)
  • Method for extracting tea polyphenol in tea residues by using IL (ionic liquid) assisted ATPS (aqueous two phase system)
  • Method for extracting tea polyphenol in tea residues by using IL (ionic liquid) assisted ATPS (aqueous two phase system)

Examples

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Effect test

Embodiment 1

[0018] The method for extracting tea polyphenols in tea dregs by using an ionic liquid-assisted two-phase system is as follows:

[0019] Dry and pulverize the tea dregs, and collect 50-mesh tea powder.

[0020] Weigh 5 mL of 50% ethanol solution and 1.25 g of ammonium sulfate, and dissolve with ultrasonic assistance for 15 min to form an aqueous two-phase system. Add 0.143g of tea powder (the ratio of solid to liquid is 1:35), transfer to an ultrasonic extractor, and extract at 45°C for 10min at an ultrasonic power of 540w. After centrifugation, absorb the upper phase layer to obtain the tea polyphenol extract, wherein the tea polyphenol extraction yield is 42.11±0.55mg / g.

Embodiment 2

[0022] The method for extracting tea polyphenols in tea dregs by using an ionic liquid-assisted two-phase system is as follows:

[0023] Dry and pulverize the tea dregs, and collect 50-mesh tea powder.

[0024] Take by weighing volume fraction and be 50% ethanol solution 5mL, ammonium sulfate 1.25g, [C 4 mim] Cl ionic liquid 0.5g, ultrasonically assisted dissolution for 15min to form a two-phase aqueous system. Add 1.43g of tea powder (the ratio of solid to liquid is 1:35), transfer it to an ultrasonic extractor, and extract at 45°C for 5 minutes at an ultrasonic power of 540w. After centrifugal separation, absorb the upper phase layer to obtain the tea polyphenol extract, wherein the tea polyphenol extraction yield is 75.52±0.75mg / g.

Embodiment 3

[0026] The method for extracting tea polyphenols in tea dregs by using an ionic liquid-assisted two-phase system is as follows:

[0027] Dry and pulverize the tea dregs, and collect 50-mesh tea powder.

[0028] Take by weighing volume fraction and be 50% ethanol solution 5mL, ammonium sulfate 1.25g, [C 6 mim]Cl ionic liquid 2.5g, ultrasonically assisted dissolution for 15min, to form a two-phase aqueous system. Add 1.25g of tea powder (the ratio of solid to liquid is 1:40), transfer it to an ultrasonic extractor, and extract at 45°C for 10 minutes at an ultrasonic power of 540w. After centrifugation, absorb the upper phase layer to obtain a tea polyphenol extract, wherein the tea polyphenol extraction yield is 67.19±1.74mg / g.

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Abstract

The invention discloses a method for extracting tea polyphenol in tea residues by using an IL (ionic liquid) assisted ATPS (aqueous two phase system). Tea residues are used as a raw material, an ethanol/ammonium sulfate ATPS is used as an extraction system, an IL is used as an assisting reagent, the extraction system and the assisting reagent are mixed to form the ATPS, after the raw material andthe ATPS are mixed in the solid-liquid ratio being (1:25)-(1:65), ultrasonic extraction and centrifugal separation are performed, a supernatant liquid of the ATPS is absorbed, and a tea polyphenol extraction liquid is obtained. The one-time extraction efficiency of tea polyphenol is higher than that with an ethanol solution, the content of catechin in the extraction liquid is higher, the oxidationresistance of the extraction liquid is not affected, fewer solvents are required, and the IL can be recycled.

Description

technical field [0001] The invention relates to a method for extracting tea polyphenols, in particular to a method for extracting tea polyphenols in tea dregs by using an ionic liquid-assisted two-phase system. technical background [0002] Tea polyphenols is the general term for polyphenols in tea biomass, mainly composed of catechins, anthocyanins, flavonoids, flavonols and fenacs, among which catechins account for 65% of tea polyphenols. ~80%. Tea polyphenols are the most important active substances in tea, and are the main components that form the color and aroma of tea. It has important application prospects in the fields of chemical industry and plastics. [0003] The extraction of tea polyphenols mostly uses tea leaves as raw materials, and the cost is relatively high. At present, the annual consumption of tea in the world exceeds 4.5 million tons. During the process of planting, production, processing and consumption of tea, there will be a large amount of waste s...

Claims

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Application Information

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IPC IPC(8): A23F3/16
CPCA23F3/163
Inventor 李明春辛梅华龚新怀
Owner HUAQIAO UNIVERSITY
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